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Filtrate of cultured dermatophyte and candia albican was incubated with human keratinocyte,the level of growth factor and the effect were comparatively analysed.

取浅部真菌和白念珠菌培养滤液与原代培养人类角质形成细胞孵育,比较表皮因子值及其对角质形成细胞生长的作用。

Dystrophic epidermolysis bullosa is a group of genetic disease caused by loss-of-function mutations in the type Ⅶ collagen gene. The clinical phenotype is characterized by the blistering and scaring of the skin from trauma and there is increased complication of squamous cell carcinoma, which is the major cause of death in patients with DEB.

营养不良性大疱性表皮松解症是一种由于人体Ⅶ型胶原基因突变或缺失所引起的遗传性疾病,临床特征为创伤诱发的水疱和瘢痕,并发症以恶性鳞状细胞癌多见,是导致患者死亡的主要因素,目前仍无有效的治疗方法。

AIM: To explore the inhibitive effects of ectogenesis nitric oxide on the proliferation of neural stem cells in mice. METHODS: NSCs were cultured in medium containing EGF, and the medium was supplemented with SNP as a donor of NO in vitro.

用含表皮生长因子的条件培养基原代培养NSC,在培养基中加入不同浓度的硝普钠作为NO的体外供体。

Results Diagnosis of sebaceous carcinoma demanded immunohistochemistry, and rare parotid sebaceous carcinoma was still distinguished with clear cell carcinoma, grume epidermis carcinoma and acinic cell carcinoma in pathology. By Follow-up of the all patients over 5 years, survival rate for 5 years was 71.4%(20/28), primary focus recurrence in 10 cases, in which, neck lymph node metastasis was discovered in 3 cases, and one was performed operation once again. Four cases lost communication; 2cases died of distant metastasis; 2cases died of other diseases.

结果 皮脂腺癌确诊需要免疫组化检测,而罕见的腮腺皮脂腺癌还需和透明细胞癌、黏液表皮样癌和腺泡细胞癌鉴别,全组病例5年以上随访,5年生存率可达71.4%(20/28),5年内原发灶复发10例,其中,3例伴有区域淋巴结转移而再次手术,4例失访;2例死于远处转移;2例死于其他疾病。

About 4 days post amputation, cells in some areas in the out epidermis and inner parietal peritoneum near the end of the stump are induced by injury to dedifferentiate into stem cells and then proliferate forming stem-cell masses, which then invaginate into the interior dermis. The dermal cells are then induced by those invaginating stem-cell masses to dedifferentiate.

从第4 d起,近创伤处的表皮层和体腔上皮中部分细胞发生了脱分化形成了干细胞,并增殖形成了干细胞团,然后向真皮层内陷,并诱导真皮层组织发生脱分化,最终在残腕体壁的不同部位形成了众多干细胞原基,是进一步再生所需的细胞库。

Methods: The basic fibroblast growth factor-based need to establish normal mitogen epidermal melanocyte cell culture, MTT method aloesin on black ...

利用碱性成纤维细胞生长因子为主要有丝分裂原建立正常人表皮黑素细胞培养系,MTT法测定芦荟苦素对黑。。。

Answer: Shoots the frequency wave is the wave band is the 40.67MHZ electromagnetic wave, causes through the different technique to shoot the frequency wave penetration surface layer base first floor the melanophore, depth of penetration 2mm-20mm, resonates the principle through a minute small water-kettle to cause the organization to heat up to the threshold value temperature 65 ℃-75 ℃, both affects to the collagen organization, and may affect to the fat, achieved dispels wrinkles the skin, dissolves the effect which the fat loses weight, does not have any damage to the epidermis.

答:射频波是波段为40.67MHZ的电磁波,通过不同的手法使射频波穿透表层基底层的黑色素细胞,穿透深度2mm-20mm,通过分水子共振原理使组织加热至阈值温度65℃-75℃,既作用于真皮层胶原组织,又可作用于脂肪,达到祛皱紧肤、溶脂减肥的功效,对表皮无任何损伤。

In treatment group, there was no progressive necrosis in stasis zone, and at 24 h post injury, capillary dilation, cell edema and inflammatory infiltration were lessened significantly. In control group, 2 wounds had progressive necrosis (accounted for 10% of the total). Cell edema, inflammatory infiltration and capillary thrombosis were serious, Until 72 hr post injury, cell edema did not subside and necrosis of the dermis worsened. 2. Pathological assessment of the stasis zone tissue: As compared with that in control group, in treatment group the dermis structure in stasis zone was intact and the collagenous fiber bundle was normal.

结果:①创面细胞形态学改变:伤后8h毛细血管扩张、细胞水肿,炎性浸润最明显;治疗组中央淤滞区无进行性坏死,伤后24h后毛细血管扩张、细胞水肿、炎性浸润减轻;对照组2个创面呈进行性坏死(占10%),中央淤滞区细胞水肿、炎性浸润明显,毛细血管血栓形成,伤后72h水肿仍明显,真皮坏死加重;②淤滞区组织病理学评分:治疗组与对照组相比,治疗侧淤滞区的表皮结构相对完整、胶原纤维束相对正常,粒细胞浸润小于5个/400倍视野。

The archesporial cell lies under the nucellus epidermis, which develops into a functional megasporocyte.

孢原细胞位于珠心表皮之下,直接起大孢子母细胞的功能。

These ingredients includes epidermal growth factor, hepatocyte growth factor, dimethyl sulfoxide, phenobarbital sodium and all trans-retinoic acid. We isolate and culture primary 10-weeks-old human fetal hepatocytes, observe hepatocytes form and its function markers. Inoculate hepatocytes with HBV Dane particles to observe if HBV susceptibility of fetal hepatocytes can be induced in vitro. To detect HBV covalently closed circular DNA within hepatocytes, we improve the existent PCR method.

基于上述假设,我们设计了10种培养条件,在自行设计的无血清培养基中添加不同的细胞分化诱导物,包括表皮生长因子、肝细胞生长因子、二甲基亚砜、苯巴比妥钠、全反式维甲酸等,分离孕10周龄人胎肝细胞进行原代培养,观测肝细胞形态及功能指标变化,并定时给予HBV感染,观察能否在体外诱导胎肝细胞出现HBV易感性;为检测肝细胞内HBV共价闭合环状DNA(covalently closed circular DNA,cccDNA),我们改进了现有的PCR检测法。

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