原肠

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

Since dominant negative Rac1 or Cdc42 failed to affect cytokinesis, it was concluded that only Rho is required.

再加上dominant-negative Rac1 和Cdc42 并会造成前述的现象，所以我们推只有Rho 对早期斑马鱼胚的及原肠化是必须的。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

结果表明：黄口荔枝螺卵囊高度平均为7.33 mm，卵粒数量平均为165粒；黄口荔枝螺的早期发育属间接发生型，受精卵呈椭圆形，卵裂为盘状卵裂；在卵囊内，胚胎发育包括卵裂期、囊胚期、原肠胚、膜内担轮幼虫、膜内面盘幼虫；幼虫发育包括面盘幼虫、后期面盘幼虫和匍匐幼虫。

In zebrafish, the expression of fez becomes detectable at the anterior edge of the presumptive neuroectoderm by 70% epiboly.

本文用整体原位杂交的方法揭示了在斑马鱼胚胎发育过程中，Fez基因的表达最早出现在原肠期胚胎头部未来的神经外胚层细胞中，此时胚盘仅下包约70％。

Fibroblast growth factor from the extraembryonic endoderm (hypoblast, a cell layer unique to amniotes) directs the expression of Wnt planar-cell-polarity pathway components to the intercalation domain.

尤其是，这项工作显示了一个原肠胚形成之前的早期插入事件，它可能是回答关于羊膜动物原始条纹是怎样从远古胚孔演化而来这样一个经典问题的关键。

GFP expression started during gastrulation in the axial hypoblast layer. During segmentation, GFP was detected in the notochord but not in the foor plate.

GFP在原肠作用期就开始表达，主要发生在中轴下胚层中；在体节形成期，GFP在脊索细胞中表达，但未发现在神经底板细胞中表达。

Transcripts of zebrafish Hspa5 are present in fertilized eggs, suggesting their maternal origin. Zygotic expression of Hspa5 starts during midgastrulation and occurs in the hypoblast cells. It is specifically expressed in the hatching gland and the notochord during segmentation.

结果表明，Hspa5 mRNA在卵子中就存在，是一个母源基因；其合子基因的表达开始于原肠中期的下胚层，在体节期发生在脊索和孵化腺中。

A certain spot in the gastrula begins to act on surrounding cells, thus inducing fast cell division, local expansion, folding, invagination, tubular structures, inclusions, etc.

在原肠胚里某一斑点开始在周围的细胞行动，因此导致快速的细胞分裂、地方扩展、可摺叠、反折处、管状结构、胞吞等。

The results showed that water content decreased first, then increased in the gastrula stage of embryonic development, reached the highest content at the second phrase larvae stage and then began to decrease from the third phase larva stage. The content of lipid in the dry weight increased with the embryonic development stages, but decreased at intramembranous nauplius stage, the second phase larva, and the infant stage of larvae development. The rate of lipid in wet weight decreased and the content of C20:4w6、20:5w3 and C22: 6w3 decreased.

结果显示，黑斑口虾蛄在胚胎和幼体的发育过程中，水的体积分数先降低，至原肠胚期开始升高，到第二相幼体期达到最高，之后又有所回落；脂类占组织干重的比率呈升高趋势，但分别在膜内无节幼体、第二相幼体和仔虾蛄期略有降低；脂类占组织湿重的比率呈降低趋势；花生四烯酸、EPA和DHA的体积分数都呈降低趋势。

With Mab 2A3, the first positive cells were detected at the limb bud stage of the embryos. Distinguishable positive haemocytes with a diameter of about 7μm appeared in embryonic-nauplius. At the following nauplius I stage, configuration of haemocytes became more integrated and clear. Their diameters were almost similar to those of haemocytes from adult shrimp. With Mab 1H11, the first positive granular haemocytes were identified at the stage of embryonic-nauplius which existed in clusters with positive punctate fluorescence of granules distributed around the periphery of granular haemocytes.

通过对包括囊胚期、原肠胚期、肢芽期、膜内无节幼体期和无节幼体Ⅰ期在内的中国对虾胚胎的间接免疫荧光检测，最早在肢芽期胚胎中检测到2A3阳性信号；在膜内无节幼体期，2A3阳性血细胞平均直径约7μm，聚集成簇；在无节幼体Ⅰ期，血细胞形态更加完整清晰，平均直径已与成体对虾血细胞相似。1H11阳性大颗粒细胞最早在膜内无节幼体期胚胎中出现，这些细胞同样成簇存在，在其细胞质外围可见点状荧光。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。