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Two pairs of primers were designed based on the sequence of ovis aries preprochymosin cDNA reported in GenBank.The preprochymosin cDNA was obtained from toltal RNA isolated from the abomasum of 5-day-old Xinong Saanen Goat by RT-PCR method and then cloned successfully in E.coli.

参照GenBank登录的绵羊凝乳酶原前体cDNA序列设计引物,以新生5d的西农萨能羊皱胃组织总RNA为模版,通过RT-PCR方法获得凝乳酶原前体cDNA,克隆测序后进行序列比对。

METHODS: BMSCs, CCs, and ADAS were primary-cultured from goat bone marrow following trypsin digestion and amplified in monolayer.

抽取羊骨髓,胰酶消化后获得原代骨髓间充质干细胞细胞、软骨细胞和脂肪源性成熟基质细胞,单层培养扩增。

Wugalj"originally meant the beautiful curly horn of argali and now the curly patterns of any shape, while the other patterns are called"He.

乌嘎拉吉"原指盘羊卷曲的犄角,因为盘羊的犄角卷曲好看,所以一般类似犄角形卷曲纹样称为"乌嘎拉吉"。其他图案被称为"贺。

The research on placenta through modern science show that its important ingredient is immuneglobulin and sneeiyne, which is the anti-aging gene, enzyme, which has high appliance value, and interferon, which protein can reach more than 80%, amino has rich resources、full ranges、and reasonable ration, which fat is less than 10%, and also has rich lecithin, cephalin, lipopolysaccharide, a variety of vitamins and trace elements,in addition, it has transfer factor, protein active factor, collagen element, organic phosphorus compound, amniotic acid and nucleic acid compound and so on.

采用现代科学对羊胎素进行分解,发现其重要成份是免疫球蛋白和延缓衰老基因sneeiyne具有很高应用价值的酶和干扰素,其中蛋白质的含量在80%以上,氨基酸含量丰富,种类齐全,配比合理,脂肪含量小于10%,还含有丰富的卵磷脂,脑磷脂,脂多糖,多种维生素和微量元素,此外,还有转移因子,蛋白质活性因子,胶原蛋白素,有机磷复合物,羊膜酸和核酸化合物等。

The results made it possible that in the traditional taxonomy alpaca was breeding camel after domestic guanaco and provided molecular basis for the traditional taxonomy .

结果表明,羊驼与骆马和美洲驼存在的遗传差异相对比羊驼与原驼大,为传统分类学中的羊驼是原驼驯化以后的一种家养驼的提法提供了分子学依据。

The antigen trait and immunogenicity of E. ovi were analyzed using SDS-PAGE and western blotting, then the E. ovis antigen was used as a diagnostic antigen to detect 64 serum samples by ELISA.

将提取的羊附红细胞体抗原进行SDS-PAGE电泳及免疫印迹分析,并以此抗原为诊断杭原,用ELISA方法对64份羊的血清样本进行了初步检测。

The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E. ovis. Of the 64 samples detected, the positive rate was 66.7%(42/64), 22.9% higher than that of direct microscopic examinerion.

结果表明,56kDa和26kDa的羊附红细胞体抗原具有较好的免疫性,而且不与羊附红细胞体阴性血清和羊支原体病阳性血清发生交叉反应,具有较好的特异性;ELISA检测的64份血清样本的阳性率为66.7%,比涂片镜检高22.9个百分点。

PartⅢImmunoresonance Scattering Spectral Analysis of Trace Fibrinogen with Labeling Gold-nanoparticle 9.0 nm gold nanoparticles were prepared by the trisodium citrate and used to label goat anti-human fibrinogen.

在pH 6.2的Na2HPO4-NaH2PO4缓冲溶液中,金标羊抗人纤维蛋白原与纤维蛋白原产生特异性结合,在PEG作用下,羊抗人纤维蛋白原包裹的金纳米微粒释放出来并发生了聚集,体系在580nm处的共振散射峰增强。

The formula of instant powder with sheep bone collagen polypeptides for children was studied. Several powdered vegetables and oat flour were added into the powdered milk and pulse flour too.

以奶粉、豆粉为基料,在添加羊骨胶原多肽的同时,适当添加各种蔬菜粉、燕麦粉及其他辅料,生产儿童专用型羊骨胶原多肽速溶粉。

Methods The ELISA plate was firstly coated with Mycoplasma hyopneumoniae , and then successively incubated with rabbit anti Mycoplasma hyopneumoniae serum and goat anti rabbit IgG biotin to form the solid biotin, which competed with the biotin in the solution for the limited streptavidin horse radish peroxidase conjugate.

酶标板先用猪肺炎支原体包被,再依次用兔抗猪肺炎支原体抗血浆、羊抗兔IgG -生物素温育形成固态生物素,同溶液中的生物素竞争有限量的链霉亲和素 HRP。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

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