原细胞

To rid themseles of the chitin, the mice used the same strategy they use to respond to other allergens, in which interleukin-4 and IL-13 cells including eosinophils and basophils enter lung tissue to attack the inader.

为了自我清除甲壳素，小鼠使用了对付其它变应原同样的策略，包括白介素－4和白介素－13细胞包括嗜酸性细胞、嗜碱性细胞进入肺组织攻击侵入物。

Methods BHK cells were seeded into 96 plates to be cocultured with these solutions.

方法将BHK-21细胞分别种植于类人胶原蛋白、壳聚糖、透明质酸以及共混溶液中，在倒置显微镜下观察细胞的黏附和生长情况，并利用MTT方法检测种植后1～5 d细胞的增殖情况。

Essel formation in itro was inhibited with mAbs against TNF-alpha, which, when secreted by cocultures of melanoma cells with human monocytes, induced endothelial cells under collagen gels to form branching, tubular structures.

在体外，TNF-alpha单抗能抑制血管形成，而当肿瘤细胞与单核细胞共培养时能分泌TNF-alpha，使胶原下的内皮细胞形成分枝和管状结构。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Methods: the chondrocytes of the rabbit condylar were derived from rabbit,and were cultured to the second generation in vitro,then hgfcdna transfected the cells.the capacity of cell proliferation was tested by mtt,the deferences of the type ii collagen were tested by sabc immunohistochemical of the type ii collagen after infected;the mrna levels were tested nd so on the mrna levels were tested by in situ hybridization of the type ii collagen between the transfection group and comparison group .

取兔膝关节软骨细胞体外培养至第二代，hgfcdna转染软骨细胞，空白质粒组为对照组；噻唑蓝检测软骨细胞的增殖能力，sabc免疫组化及原位杂交法测其ii型胶原在转染前后的变化与mrna的表达。

Immunoelectron microscopy showed that the distinct ultrastructure of fibroblast and the group, spot gold particle specificity distribution shape. The positive markers of CTGF were mainly located in the RER,while there are expression in RER ribosome, Cf, ECM, desmosome connection, euchromatin,et al.

免疫电镜标记显示成纤维细胞细胞超微结构清晰，金颗粒呈团状、点灶状分布，特异性强，CTGF蛋白阳性标记主要位于粗面内质网，粗面内质网核糖体、胶原纤维、细胞外基质、桥粒连接、常染色质等部位也有表达。

Objective: Basic Fibroblast Growth Factor is a mitogen for many cell types, including osteoblasts and chondrocytes. It can stimulate angiogenesis and oateoblast gene expression.

目的：碱性成纤维细胞生长因子是许多种细胞的丝裂原，其可促进血管的生成及成骨细胞基因的表达。

VEGF positive stainings of endothelialcells and tumor cells were higher in pheochromocytomas than those in normalcontrols.

1。建立了人嗜铬细胞瘤细胞的原代培养方法，并证明其具有嗜铬细胞瘤的分泌和表达功能。2。

Rat primary hepatocytes cultured on chitosan microcarrier were observed by using phase contrast microscope and scanning electron microscope.

用其进行原代大鼠肝细胞培养，利用相差显微镜和扫描电镜对细胞形态进行观察，测定细胞的代谢活性。

In order to elucidate the regulation mechanism of RU5 region to BFV gene expression, BFV3026 provirus DNA was used to construct the plasmids containing different deletion of R region, which were cotransfected with luc report gene locatied behind the IP promoter to BL12 cells, and luciferase activities was assayed, confirming that U5 region could repress the initiation function of LTR as well as IP. The BFV structure genes with different deletion of R region were cloned closely behind to heterogenous CMV promoter, then transfected to 293T cells, RT activity was performed, testifying the R region was required for BFV pol gene expression, and also the function domain was identified within the 100n.t. sequence at the 5′end.

以牛泡沫病毒(Bovine foamy virus， BFV)中国株BFV3026原病毒DNA为材料，构建R区系列缺失质粒，通过对其转染细胞中RT水平及对缺失质粒与luc报告质粒共转染细胞中萤火虫荧光素酶活性的测定，确立U5区对于BFV3026两类启动子LTR和IP均具有负调控作用；同时将带有不同R区的BFV3026结构基因片段克隆于异源启动子CMV之下，通过对其转染细胞293T中RT酶活性的测定，确立R区对于病毒结构基因pol的表达具有一定的调节作用，并将其功能区域初步界定在R区5′端100bp内。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。