原细胞

P＜0.01. 28d, q=4. 0452, p＜0.05 . Corresponding pathologic result shows that the corneal stromal of height edema increase thickness to have infiltration of inflammation cells with hyperplasia of fibroblast cell and large vessel. The collagen fiber in stromatic layer is dissolved and destroyed seriously, suggesting that in addition to HSV-1's direct destructuring effect, inflammatory cells that might play an important role in the pathology of HSK induce the immune-pathological reaction.

相应的组织病理学研究结果显示（UV-B 14天）：病毒对照组角膜基质层高度水肿、增厚，并有大量中性粒细胞等炎性细胞浸润、成纤维细胞和新生血管增生，胶原纤维肿胀、分解等，说明除HSV-1对角膜组织直接破坏外，炎性细胞介导的免疫病理反应在复发性HSK发病机理是重要的因素。

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

Objective: Mast cell tryptase has been implicated in regulation of skin fibroblast, lung fibroblast and hepatic stellate cell proliferation and collagen synthesis.

目的：肥大细胞类胰蛋白酶已被证明在皮肤、肺成纤维细胞和肝星型细胞增生和胶原合成中具有重要作用。

The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

人工感染雏鹅实验中，雏鹅临床表现为高发病率和高死亡率，食欲和饮水减少，拉白色、黄绿色稀粪，伴有神经症状；常规组织学观察，脾脏和胰腺肿大，有白色坏死斑点，肠道出血和坏死，肝脏肿大变性，肾脏肿大变性有尿酸盐沉着等；用特殊染色和组织化学方法首次对人工感染鹅副粘病毒的雏鹅的重要脏器进行研究，结果表明：胰腺中发现病毒包涵体，肠道中大量纤维素以及胶原纤维增生，肾脏、肺脏大量含铁血黄素沉着，免疫器官如脾脏和淋巴结中T、B淋巴细胞大量减少；电镜下多种细胞呈现线粒体水肿，多种实质细胞的染色质固缩，染色质边集，特别是细胞内容物如脂肪滴增多的病理变化特征。

Objective To investigate the mechanisms of hair cell generation and regeneration, a primary culture system of utricular sensory epithelial cells of the rat was established.

目的 建立大鼠椭圆囊感觉上皮细胞( utricular sensory epithelial cell， USEC )的原代培养体系，为研究毛细胞再生机制提供大量来源一致的细胞。

RESULTS:①Morphology of skeletal myoblasts: After 48-72 hours primary culture, the cells were completely attached and gradually extended in the shape of spindle with the round nucleus, paralleled along their longitudinal axis and appeared chromatospherite. After 5-day culture, the cells stretched out one or several apophyses.

结果：①骨骼肌卫星细胞的形态：原代培养48~72 h细胞完全贴壁，呈梭形或纺垂形，长轴平行排列，胞核圆形，核仁明显。5 d时细胞可伸出一个或数个突起。

Blood samples were drawn at 30 minutes before and 0 minute, 12 hours, 24 hours, 36 hours and 48 hours after the race from 14 subjects' forearm veins. PHA and LPS were used for assessing lymphocyte proliferation; lyses of 51Cr labeled K562 target cells were used for assessing natural cytotoxicity. Results showed that the lymphocyte proliferation stimulated by PHA and LPS was decreased at least for 24 hours; the natural killer cytotoxicity is increased at 0 minute and decreased at least 48 hours after the race.

本研究以14位一般大学男生为研究对象，血液样本於14位受测者跑步前30分钟及跑步后0分、12小时、24小时、36小时与48小时於受测者前手臂静脉抽取，以PHA及LPS有丝分裂原刺激淋巴细胞，评估周边血液中淋巴细胞增殖功能；以自然杀手细胞分解51Cr标记之K562标的细胞释出51Cr之总量，评估自然杀手细胞毒杀活性。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人EG细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体MAB1281作为示踪剂，通过免疫组化方法检测移植细胞的分布。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人eg细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体mab1281作为示踪剂[1]，通过免疫组化方法检测移植细胞的分布。

METHODS: Human bronchial epithelium cells were isolated and cultured from 18 patients undergone pueumonectomy in hospital. Interleukin 4 or interleukin 6 at concentration of 10 μg/L was used to treat these cultured cells, and Iamil was measured by whole cell patch clamp techniques.

体外分离培养气道上皮细胞，经过终浓度均为10 μg/L的IL-4、IL-6处理，用全细胞膜片钳技术记录原代培养的气道上皮细胞中Iamil。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。