原细胞

To study the role of FoxO1 on myoblast proliferation and differentiation and the transformation of muscle fiber type in porcine,here we use yorkshire as experimental materials and detect the expression of genes related myogenesis and muscle fiber type in pporcine myoblast that was knockout or activated FoxO1,adopting the technology of RNAi,real time-PCR and Western blotting et al..

为探讨FoxO1对猪成肌细胞增殖分化及肌纤维类型转化的作用及机理，本试验以大白猪为研究材料，利用RNAi、real-time PCR、免疫细胞化学和Western blotting等技术，通过在原代培养的猪成肌细胞中敲除和激活内源活性FoxO1，检测肌肉形成与肌纤维类型相关基因的表达。

In contrast, 11/15 primary glioblastomas contained a significant CD133 subpopulation that displayed neurosphere-like, nonadherent growth and asymmetrical cell divisions yielding cells expressing markers characteristic for all three neural lineages.

相反，15个原代胶质母细胞瘤中有11个含有明显的CD133阳性亚群，呈神经球样，非附着性生长和非对称性细胞分裂，后者产生表达所有三种神经细胞系的特征标志物的细胞。15

To eplore the relationship between Dynein and the formation of meiosis error of oocytes, the immature mouse oocytes were in vitro cultured with a known dynein ATPase activity inhibitor- sodium orthovanadate to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the alteration of the cyclin B1 mRNA level.

为探讨Dynein与卵母细胞减数分裂错误形成的关系，采用小鼠卵母细胞体外培养成熟技术和半定量RT-PCR方法，分别检测Dynein抑制剂-原钒酸钠对卵母细胞成熟率和cyclin B1基因转录水平的影响。

Histologicat results gave, proof to osteogenetic ability of these combined collagen membranes by new bone quantity underneath the membrane in HE and Masson's trichrome stain.

复合rhBMP-2的纤维蛋白凝胶具有特殊的表面形貌，结合细胞培养发现，大鼠成骨细胞在复合膜上具有良好附着和增殖，复合胶原膜细胞相容性良好。

The CFb in blank control group was added with IMDM containing 10% FBS and those in Ang Ⅱcontrol group with Ang Ⅱ.The CFb in the 3 test groups were induced with Ang II,then added with Tau at dosages of 40,80 and 160 mmol/L respectively. Determine the proliferation of CFb by MTT method, the collagen content by oxyproline kit, the transforming growth factor- TGF-β1 (TGF TGF-β1) content by ELISA, the cell cycle by flow cytometer, the CFb NO content by nitrate reductase method, the iNOS activity by spectrophotometry, and the expression of iNOS protein by IFA.

胰酶消化法分离培养新生大鼠CFb，用Ang Ⅱ诱导促进其增殖，采用MTT法检测细胞增殖；羟脯氨酸试剂盒检测胶原含量；ELISA法测定转化生长因子β1(TGF-β1)蛋白的含量；流式细胞仪检测细胞周期；硝酸还原酶法、分光光度法和免疫荧光法检测CFb NO含量、一氧化氮合酶活性和iNOS蛋白的表达。

Our results showed that the RnR was localized in the perinuclear zone and plasma membrane of the MCs.

本文旨在观察RnR在体外培养的大鼠肾小球系膜细胞(mesangial cells， MCs)和肾脏中是否表达，及其表达的细胞部位，并用RnR的多肽阻断剂肾素原&柄区肽&(handle region peptide， HRP)与RnR结合后观察受体复合物进入细胞的过程与定位。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果：HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化，膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变；Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少；免疫组化结果表明3组均有不同程度的caspase-3表达，损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01)，损伤上段及下段caspase-3表达较损伤节段为少(P.01)，但均高于正常对照组(P.01)。

The results showed that the non-embryonic callus were cultured on the MS medium contained 4.0 mg · L-1 2,4-D and 0.5 mg · L-1 6-BA, embryogenic cells appeared in it. Then the single embryogenic cells formed a proembryo composed of many cells through cell division.

结果表明，非胚性愈伤组织转入到MS + 4.0 mg · L-1 2，4-D + 0.5 mg · L-1 6-BA培养基培养后，愈伤组织中先出现一些胚性细胞；然后单个胚性细胞分裂形成一个由多细胞组成的原胚。

RESULTS: HE stained cells were fusiform-shaped with pseudopodia, round or oval nucleus. The cytoplasm was blue after stained with the toluidine blue. The immunohistochemical test revealed that there existed positive expression of type Ⅰ collagen in the cells. In the presence of 10% calf serum, IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.

结果：苏木精－伊红染色细胞多为梭形，有伪足伸出，细胞核为圆形或椭圆形；甲苯胺蓝染色，胞浆为深蓝色；免疫细胞学方法检测表明纤维环细胞有Ⅰ型胶原表达；在体积分数为0.1的血清条件下，胰岛素样生长因子Ⅰ能提高细胞的增殖活性，并且在有效浓度范围内呈剂量效应关系。

The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44%.

采用30mJ/cm^2、60mJ/cm^2、90mJ/cm^2剂量UVB照射体外培养的原代人皮肤成纤维细胞，以中药单体EGCG和黄芩苷进行干预处理，倒置相差显微镜观察细胞受损程度，以甲唑盐比色试验检测细胞增殖活性，酶联免疫吸附试验检测上清液TNF-α、IL-6分泌量。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。