原细胞

The digestion process includes the following steps: adding 0.15 % concentration collagenase II solution compounded with DMEM into blood vessel smooth muscle tissue block and acting in water bath for 1.5 hr until forming floccular tissue; cleaning with 0.02 % EDTA solution, adding 0.125 % trypsase solution and water bath vibrating to digest at 37 deg.c to obtain dispersed smooth muscle cells; terminating enzyme action with 10 % new born ox serum culture liquid; regulating cell concentration with 20 % new born ox serum culture liquid to 50,000-500,000/L, inoculation, and culturing in culture tank with 5 % CO2 and first 37 deg.c to cell fusion of 70-80 %; and subculturing conventionally.

向血管平滑肌组织块中，加入用DMEM配制的0.15％胶原酶II溶液，水浴箱中作用1.5h，至组织呈絮状，用0.02％的EDTA清洗，然后，加入0.125％胰蛋白酶溶液，于37℃水浴振荡消化，即可得到分散的平滑肌细胞，用10％新生牛血清的培养液终止酶的作用。加入含20％新生牛血清的培养液，调节细胞密度为5×104～5×105个/L，接种，放入5％CO2、37℃培养箱中培养。当细胞达到70％～80％融合时即可按常规方法传代。

But soon some of the cells in the gastrula become specialized, a division of labor has set in among the cells which soon deprives them of the capacity to change their own structure and function back to the original state.

但一些细胞在原肠胚很快成为专门的，工作的分配已经设置在细胞之中，细胞很快剥夺他们自己的能力改变他们自己的结构和作用回到原始的状态。

The Collaborative Program in Developmental Biology is primarily dedicated to research and education in Developmental Biology including amphibian gastrulation, molecular control of hematopoiesis, lymphocyte lineage commitment and cell development, hematopoietic stem cells, lymphocyte development, cell enlargement and cell differentiation, vertebrate embryogenesis, developmental neurobiology.

动物学系发育生物学合作研究组主要致力于两栖动力原肠胚形成，造血作用的分子控制，淋巴细胞血统定型和细胞发育，造血干细胞，淋巴细胞发育，细胞生长和细胞分化，脊椎动物胚形成，发育神经生物学等。

The Collaborative Program in Developmental Biology is primarily dedicated to research and education in Developmental Biology including amphibian gastrulation, molecular control of hematopoiesis, lymphocyte lineage commitment and cell development, hematopoietic stem cells, lymphocyte development, cell enlargement and cell differentiation, vertebrate embryogenesis, developmental neurobiology.

动物学系发育生物学合作研究组主要致力于两栖动力原肠胚形成，造血作用的分子控制，淋巴细胞血统定型和细胞发育，造血干细胞，淋巴细胞发育，细胞生长和细胞分化，脊椎动物胚形成，发育？？？经生物学等。

The shortage of this way was that feeder cells could be passaged together with hESCConclusion HAFi could replace MEF as feeder layer to support the long-term culture oftwo Chinese human embryonic stem cell lines. This could remove the cells of animal originand heterogenetic pollution in the culture system. It also reduced the culture of primarycells and solved the instability caused by different batches of primary cells.

结论HAFi细胞可以用做两株中国人胚胎干细胞培养的饲养层，取代MEF后解决了动物源性细胞混杂和异源污染问题，省却了饲养层原代细胞的培养步骤同时解决了由不同批次饲养层差异带来的培养体系的不稳定。

After the culture under the mineralization fluid condition in about 1 wk, the compact circular group appeared in the intercellular space, simultaneously cell multiplication ability reduced compared with the conventional culture; The sodium alizarinsulfonate dyeing showed the compact and circular lightproof briquetting brown laminated shape in the mineralizd intercellular spac, I collagen immunity group dyeing was strongly positive in the coloring region.

在矿化液条件下培养1周左右，细胞间出现了致密圆形团，同时细胞的增殖能力较常规培养有所降低；茜素红染色结果显示矿化细胞间出现的致密的、圆形不透光团块呈现片状的棕染，着色区域， I型胶原的免疫组化染色强阳性。

Results The latent phase of the primary culture cells was 5-9 days, followed by 11-16 days of logarithmical proliferation; the plateau began at day 18-20. The subcultures showed 12-24 hours of latent phase, followed by 7-10 days of logarithmical proliferation, then entered the plateau (11-13 days). The hMSC expressed positive CD44 unanimously, but don′t expressed CD45. Low concentration of bFGF (10 μg·L-1) could stimulate the proliferation of hMSC significantly (P.05); while 25-100 μg·L-1 of bFGF could inhibit the ALP activity.

结果：原代培养细胞生长潜伏期5~9 d，对数增殖期11~16 d，生长平台期18~20 d；传代培养细胞生长潜伏期12~24 h，对数增殖期为7~10 d，11~13 d进入细胞生长平台期。hMSC均一表达CD44，不表达CD45.bFGF在较低浓度（10 μg·L-1）时即有显著的促进hMSC增殖的作用（P.05）， bFGF在25~100 μg·L-1对hMSC的ALP活性有较显著的抑制作用。

To mensurate the expression of MDR1 of different breast cancer cells from human breast cancer primary cells and MCF-7 breast cancer cell line,and the kill effect of chemotherapy drug with different breast cancer cells in vitro,and the change of the proporation and the expression of MDR1 of breast cancer stem cells after chemotherapy.

测定人乳腺癌标本和MCF-7中不同细胞亚群的MDR1表达情况；并测定化疗药物在体外对人乳腺癌原代细胞和MCF-7中不同细胞亚群的杀伤作用，以及在化疗药物干预后，乳腺癌干细胞的含量和MDR1表达的变化情况。

Results: the number of th positive cells and the viability of mesencephalic cells significantly decreased while the number of inos cells increased in the development of apoptosis induced by mpp+.

结果：在mpp+诱导的中脑原代细胞凋亡中，mpp+组th阳性细胞数及细胞存活率均显著低于各egb761组及阳性对照组(p.01)。

Chloride channels have been identified in vasucular smooth muscle cells. These channels have been shown to be involved in myogenic tone regulation and neuromuscular transmission in various vascular beds. However, whether the chloride channels are responsible for the formation of excitatory junction potentials of the smooth muscle cells in the spiral modiolar artery remains elucidated.

许多研究已证实，血管平滑肌细胞膜上存在氯离子通道，进一步研究发现氯离子通道不仅参与调节平滑肌细胞的肌原性紧张，而且参与了许多种类血管床的神经平滑肌细胞之间的信息传递，但Cl-通道及氯离子通道阻断剂对耳蜗螺旋动脉细胞兴奋性接头电位(excitatory junction potential，EJP)是否有影响，目前还不清楚。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。