原细胞

Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining and a characteristic "ladder" of DNA fragments was elicited by agarose gel electrophoresis; Western blot analysis revealed that caspase-3 was activated by the loss of caspase-3 proenzyme (32kDa) and the appearance of its 20kDa subunit, and that along with the apoptotic process caspase-3 activity was increased concurrently.

Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化，琼脂糖凝胶电泳观察到细胞凋亡时的DNA"梯形"条带；Western blot检测结果表明32kDa的caspase-3酶原被激活，出现20kDa的亚单位活化片段，同时在细胞凋亡过程中，caspase-3活性显著增高。

In 5 μM. pseudolaric acid B-treated HeLa cells, caspase-3 proenzyme decreased followed by the degradation of caspase-3 substrates, ICAD (inhibitor of caspase dependent DNase) and PARP poly-(ADP-ribose polymerase.

土槿皮乙酸5μM作用于HeLa细胞后，caspase-3的酶原被降解，caspase-3底物ICAD(inhibitor of caspase activated DNase)和PARPpoly-(ADP-ribosepolymerase降解，caspase-3的抑制剂可部分抑制土槿皮乙酸诱导的细胞死亡，表明土槿皮乙酸诱导HeLa细胞凋亡时激活了caspase-3。

RESULTS:①Retroviral vector EGFP-pLNCX2 transfected primary chondrocytes of rabbits,and high expression of transfected chondrocytes could be obtained through preliminary screening of G418.After being screened and expressing EGFP,chondrocytes kept normal morphology,with pseudopod adhering to the wall and matrix secreting vigorously.

结果：①重组逆转录病毒载体EGFP-pLNCX2转染原代兔软骨细胞，经G418初步筛选而获得的高表达格局，可见软骨细胞经过筛选和表达绿色荧光蛋白后，保持正常的形态学，细胞伸出伪足贴壁，基质分泌旺盛。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜，含类似上皮层和固有层，但细胞层次不清；胶原膜上可见口腔黏膜上皮细胞黏附生长，并形成复层上皮样组织，但无成熟的桥粒及基底膜样结构；以Alloderm作为支架材料的构建物，出现正常上皮形态，含半桥粒及细胞指状突起等超微结构。

The blank group, the pure chondrogenic inductor group and the group of the G ranula of P enetrating Bone and Removing Pain mixed with chondrogenic inductor. We adopted pro-culture solution, pure chondrogenic induced culture solution （ TGF-β310ug/L , Dex10-7mol/L , VitC50mg/L ） and the chondrogenic induced culture solution which included the serum of the G ranula. All groups were cultivated in 50ml cell culture bottles. The effects of the G ranula of P enetrating Bone and Removing Pain on chondrogenic phenotype differentiation of BMSCs were investigated after being cultivated for 1, 2, 3 weeks, then cells observed by inverted phase contrast microscope and immunocytochemical stain.

3将第2代SD大鼠BMSCs分为空白对照组、单纯软骨诱导剂组及透骨消痛颗粒含药血清加软骨诱导剂组，采用原培养液、软骨诱导培养液（TGF-β310ug/L，Dex10-7mol/L，VitC50mg/L）及透骨消痛颗粒含药血清的软骨诱导培养液，50ml细胞培养瓶内进行培养，1、2、3周后通过倒置相差显微镜及免疫细胞化学染色等实验技术，研究透骨消痛颗粒对BMSCs诱导成软骨细胞的影响。

Cell elongation in the middle and upper middle stipe regions accounted for most of the stipe elongation but this was accompainied by cell divisions.

在原基体时期其快速生长区间菌柄细胞数为其他部位的四倍，其细胞生长主要在基部最为活跃，而非其边缘细胞。

The juice sac primordium originated from a single cell of the carpel-lary endoepidermis with partial participation of subepidermal cell just belowthe initial cell.

锦橙和温洲蜜柑的汁囊最初是从心皮内表皮的单个细胞发生的，后来原始细胞下面的细胞也发生分裂，部分参与了汁囊原基的形成。

The result indicated when the concentration of olaquindox was higher than 0.30μgmL^(-1), it could increase the depot fat of liver cell and pancreas exocrine cell of Ctenopharyngodon idellus, and interdict the biosynthysis of trypsinogen of pancreas exocrine cell.

结果表明，高于0.3μgmL^(-1)浓度的喹乙醇可显著导致草鱼肝细胞和胰腺外分泌部细胞的脂肪积累，抑制草鱼胰腺外分泌部细胞胰蛋白酶原的合成。

RESULTS: The AF cells cultured in different substrates were morphologically undistinguishable. Toluidine blue staining showed that there was also no difference between AF cells cultured on flexible silicone membranes and in plastic plates. The cells growing in different substrates expressed the same levels of collagen typeⅠ,Ⅱ and aggrecan mRNA and integrin β1. AF cells grew well on silicone membranes.

结果：种植于不同基质的纤维环细胞形态学观察无明显差别；在两种基质上的纤维环细胞甲苯胺蓝染色无差异；Ⅰ，Ⅱ型胶原及聚集蛋白聚糖mRNA及细胞膜整合素β1的表达亦无明显差异；纤维环细胞可以黏附于硅橡胶膜上生长。

The well-grown cells of the third passage were co-cultured in vitro on the nano-CS/COL scaffold. Taking simple nano-CS/COL scaffold material as control, the histocompatibility of scaffold material and cells were comprehensively evaluated by cell adherence rate, growth curve, cell activity and cycle, and scanning electron microscope observation.

取生长良好的P3代，与纳米壳聚糖-胶原纤维支架体外联合诱导培养，以单纯纳米壳聚糖支架材料为对照，通过细胞贴壁率、生长曲线、细胞活力及周期、扫描电镜观察综合评价材料与细胞的相容性。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。