原细胞

METHODS: BMSCs, CCs, and ADAS were primary-cultured from goat bone marrow following trypsin digestion and amplified in monolayer.

抽取羊骨髓，胰酶消化后获得原代骨髓间充质干细胞细胞、软骨细胞和脂肪源性成熟基质细胞，单层培养扩增。

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

METHODS: Detection of the cell proliferation by MTT method and ALP expression by enzyme dynamics method.

原代培养牙髓成纤维细胞、牙周膜成纤维细胞，用MTT法测定细胞增殖，酶动力学方法测定ALP活性。

Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, ultra structure changes, titer in 50% tissue culture infectious doses, and the alterant distribution of DNA content in cell cycle. The results showed that, under identical conditions, various degrees of CPE could be found in the Hep-3B, A549 and NIH 3T3 36h post infection of BTV-10, while there was no CPE could be observed in HEL.

随后在对BTV-10体外感染特性的研究中，我们在相同条件下，将该病毒接种于1株原代人胚肺成纤维细胞(Human embryonic lung fibroblast cells，HEL)和3株传代细胞株：人肝癌细胞(Human hepatic carcinoma 3B cells，Hep-3B)；人肺腺癌细胞(Human lung carcinoma A549 cells，A549)；鼠正常成纤维细胞(Normal mouse fibroblastscells，NIH 3T3)。

Mouse fetal tissues could be cut into uniform small pieces by scissors cutting and needle grinding, which grew well in tissue culture and great amounts of cells grew from the attached small tissue clump to form primary mouse embryonic fibroblasts monolayer.

将这些组织块培养48h，大量的梭形细胞从贴壁小组织块的边缘游离出的，细胞密度大，细胞生长旺盛，很快形成原代小鼠胎儿成纤维细胞单层。

Two days after bone marrow MSCs compounded to nano-CS/COL scaffold, bone marrow MSCs presented globular shape and were scattered; Four days later, bone marrow MSCs presented shuttle shape, extended and anchored on the surface of nano-CS/COL by pseudopods; Eight days later, bone marrow MSCs proliferated and fused each other, and they secreted a lot of extracellular matrix, then which covered most material particles.

骨髓基质干细胞复合到纳米壳聚糖-胶原纤维支架后2 d，细胞呈球形散在分布；4 d 后细胞呈梭形，延展爬行且有伪足与材料表面锚靠；8 d 时细胞增殖，相互间融合，并有大量的细胞外基质分泌，大部分材料颗粒被覆盖。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中，待细胞生长融合后在培养板上划线，造成无细胞的裸露区，分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液，每6h观察记录移行至裸露区的细胞。

objectiveto investigate the effects of yuegan capsule on ccl4 intoxicated primary cultured rat hepatocytes.methodshepatocytes injury models were induced by ccl4 in rats in vitro, and treated with yuegan capsule.structure of rat hepatocytes and effect of sdh、acpase、g 6 pase activeties and amount of hepatic glycogen were observed.

目的探讨悦肝胶囊对四氯化碳（ccl4）损伤原代培养大鼠肝细胞的保护作用。方法以ccl4诱导大鼠肝细胞损伤模型，观察悦肝胶囊对肝细胞的琥珀酸脱氢酶、酸性磷酸酶、葡萄糖-6-磷酸酶（g 6 pase）活性和糖原含量以及肝细胞形态结构的影响。

The maximum MTG activity in the culture broth was 5.04u/mL and increased by 21.8% compared with the control. With the addition of CTAB, pro-MTG was activated to become MTG. CTAB could enhance the production of pro-MTG by relieving the pr...

初步研究表明，CTAB的主要作用是促使谷氨酰胺转胺酶的酶原转化为成熟酶，因此，在发酵过程中添加适当浓度的CTAB，可使酶原快速、完全地转化为成熟的MTG，解除酶原的产物抑制作用，促进了细胞产酶。

Bcl-2 mRNA was detected by RT-PCR in all cells;（2）After normal rat and bile duct-ligated 14d rat hepatocytes were added 100 μmol/L GCDC and kept for 24 hours, cells were evaluated by FCM and TUNEL. Results:（1）Normal rat hepatocytes did not express Bcl-2 by RT-PCR technique. Bcl-2 was expressed in 7-,14-,21- day BDL rats.

分离对照组及实验组14 d组大鼠的肝细胞行原代培养后，使用100 μmol/L甘氨鹅脱氧胆酸钠作用于两组肝细胞24 h后，用FCM法测定肝细胞的凋亡比率，用TUNEL技术进行肝细胞凋亡的原位检测。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。