原细胞

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

Classification examination was put into practice for only normal cells observed.For the dubious specimen,tuberculosis-Ab,acid-fast bacteria staining,flow cytometry,CD series,gene,DNA aneuploidy,ZPP and tumor markers etc were detected. Results Tuberculosis lymph gland inflammation coverd highest rate:546 cases (36%) in which tuberculosis node was 252 cases (46.2%),lymphonode period was 184 cases (33.7%),early tuberculosis lymphagland inflammation was 233 cases (44.7%).

利用改良一次性5～10ml注射器(22G、7号注射器，外径＜0.7mm)，针吸取材、涂片、行改良瑞-姬双染技术，低倍镜查找特殊异常细胞，油镜仔细观察细胞形态结构特征，对于未见异常细胞要进行分类检查，对疑难标本，同时做结核抗体、抗酸菌染色、流式细胞仪、CD系列、基因、DNA异倍体、锌原卟啉、肿瘤标志物等检测指标。

It was shown that: Micropipette aspiration technology is an advanced artifice on the level of single cell to study the viscoelasticity、adhesion properties of HCCs to the surface coated with basement membrane and endothelial cells.

表明微管吸吮技术是一种较好的在单个细胞水平上研究肝癌细胞粘弹特性、肝癌细胞与Ⅳ型胶原和内皮细胞粘附力学特性的先进手段。

This the experiment is fostered through former acting cell, go down to the future generation cellular education, be in eventually go down to the future generation detached extract gives the cell in fluid of education of the 3rd acting cell muskrat of natural animal flavor is sweet.

本项实验通过原代细胞培养、传代细胞培养，终于在传代细胞第3代细胞培养液中分离提纯出天然动物香料麝鼠香。

A549 cell line was stimulated with LPS (10 μg/mL) and then treated with Rs504393 (10 μg/mL) for 6 hours. ALI model was established with intranasal administration of LPS (5 mg/kg) in C57BL/6J mice. RS504393 (5 mg/kg) was administered 30 min before LPS dripped nasally. IL-8, IL-1β, plasminogen activator inhibitor-1, monocyte chemoattractant protein-2, and the expressions of CCR1 and CCR2b were studied by using Realtime-RT-PCR, ELISA and cyto-flowmetry.

体外实验选用A549细胞系，应用LPS(10μg/mL)刺激合并RS504393(10μg/mL)治疗6 h后，流式细胞仪技术检测CCR1和CCR2的表达，ELISA法检测细胞培养上清液内白介素-8的浓度；体内实验选用C57BL/6J小鼠，腹腔注射RS504393(5 mg/kg)预处理30 min后经鼻滴入JPS(5 mg/kg)，LPS刺激4 h后收集BALF和肺脏标本，计数BALF内细胞总数，应用BCA法检测BALF内蛋白浓度，Realtime-RT PCR和ELISA法检测BALF和肺脏IL-1β、凝血酶原激活物抑制物-1和单核细胞趋化蛋白-2的表达。

In treatment group, there was no progressive necrosis in stasis zone, and at 24 h post injury, capillary dilation, cell edema and inflammatory infiltration were lessened significantly. In control group, 2 wounds had progressive necrosis (accounted for 10% of the total). Cell edema, inflammatory infiltration and capillary thrombosis were serious, Until 72 hr post injury, cell edema did not subside and necrosis of the dermis worsened. 2. Pathological assessment of the stasis zone tissue: As compared with that in control group, in treatment group the dermis structure in stasis zone was intact and the collagenous fiber bundle was normal.

结果：①创面细胞形态学改变：伤后8h毛细血管扩张、细胞水肿，炎性浸润最明显；治疗组中央淤滞区无进行性坏死，伤后24h后毛细血管扩张、细胞水肿、炎性浸润减轻；对照组2个创面呈进行性坏死（占10%），中央淤滞区细胞水肿、炎性浸润明显，毛细血管血栓形成，伤后72h水肿仍明显，真皮坏死加重；②淤滞区组织病理学评分：治疗组与对照组相比，治疗侧淤滞区的表皮结构相对完整、胶原纤维束相对正常，粒细胞浸润小于5个/400倍视野。

objective to investigate the influence of dit on the proliferation of human thyrocytes and compare dit with ki.to study a good kind of supplement to usi that was safe and effective.methods the human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10-8～10-3mol/l dit or ki.mtt method was employed to obtain the proliferation of cultured human thyrocytes.

取甲状腺腺瘤旁正常甲状腺组织，应用原代细胞培养技术进行细胞培养，以不同浓度(10-8～10-3mol/l)的dit和ki刺激单层培养的甲状腺细胞，采用四甲基偶氮噻唑蓝法测定各组甲状腺细胞的增殖情况。

2Cm bone defect was established in ulna of rabbits, the capacity of different therapeutic measures in bone defect repairment was determined in groups A , B (implanted BMP, TGF -β〓 and carrier) and C . It was found that the appearance of osteoblasts and formation of bone union were earlier and the number of osteoblasts more in group B than in group A. The amino acid analysis revealed that the volume of ossein was significantly higher in group B than in group A at the early stage.

分别用bBMP及载体bBMP、TGF-〓及载体和单纯载体修复兔尺骨1.2cm骨缺损的实验模型来比较三者间的成骨效果，结果表明B组比A组成骨细胞出现早、形成数量多、骨连接形成提前，骨胶原氨基酸分析在骨缺损修复早期胶原形成量B组均高于A组，且B组以形成Ⅰ型胶原为主，C组表现为一纤维形成过程。

We carried out a primary study of shear stress activated K+ channel in osteoblast by using clamp patch technology .

研究原癌基因表达与剪应力的关系对于了解细胞通过何种途径将剪应力信号的刺激传入细胞内并引起一系列的细胞应答，对于进一步认识流动剪应力作用对成骨细胞功能、代谢等产生的影响都有重要的意义。

Results: Caspase-3 mRNA was constitutively expressed at a low level in freshlyisolated CD34+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactivecaspase-3 proenzyme was detected in the freshly isolated CD34+ cells as well as during the first 3 daysexpansion with cytokines.

检测结果显示，Caspase-3mRNA在新鲜分离的脐血CD34+细胞中低水平表达，在细胞因子支持下体外培养3天，扩增的CD34+细胞中Caspase-3mRNA和蛋白质水平表达上调，但在这两种细胞中仅能检测到分子量为32KDa的非活性酶原形式的Caspase-3；随着体外培养时间的延长，在IL-3，IL-6和GM-CSF组合条件下，Caspase-3被激活，可检测到分子量为20KDa的裂解片段。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。