原细胞

Acellular allogeneic dermal matrix is the dermal replacement of heterogenous human skin in which the cellular elements have been removed by special technique and basement membrane complex have been remained. It becomes a kind of 3D porous acellular dermal scaffold. It supports fibroblast infiltration, neovascularization and epithelialization in the absence of an inflammatory response.

异体脱细胞真皮基质（acellular allogeneic dermal matrix， ADM）来源于正常的异体皮肤组织，经过特殊的理化处理去除真皮中的细胞成分，保留原有的胶原三维结构和完整的基底膜，成为一种多孔的三维膜性支架，其空间网状结构有利于细胞的增殖、毛细血管增生，加速组织的愈合过程，移植后可以迅速被血管化和自体细胞重建。

The results showed:(1) the cellule morphous and the disposition in chick embryo of PGCs were coincident no matter stained by PAS or HE staining, and HE staining could disclose the morphologic characteristics more clearly, exactly and completely;(2) genesis of blood island could be observed at the boundary of light and dark region of the extraembryonic blastoderm at about 26 hours;(3) both the blood vessel endothelium cells and free cells of the blood island were differentiated from PGCs. The generating of genuine yolk sac was at about 44-48 hours.

结果表明：①用经典的识别原生殖细胞的PAS染色方法与HE染色所观察到的PGC在鸡胚的分布及细胞形态基本一致，而HE染色较PAS法能更有助于清晰、完整、准确地识别PGC的细胞内部结构特征；②在孵化26小时的鸡胚胚外胚盘的明暗交界处可见血岛的发生，血岛发生的时间比真正的卵黄囊产生时间（大约44-48小时）要早很多；③血岛周围的血管内皮细胞与管腔中游离细胞都来自PGC。

Methods Mimicry and speculation on possible displaced amino acids in proper positions of peptide increased the biological effects of TNFR blocking peptide by compote modeling. The original peptide and its mutant were synthesized. The competitively inhibitory effects on green fluorescence protein-tumor necrosis factor fusion protein with TNFR were compared between original peptide and its mutant (receptor competitively inhibitory binding assays). The inhibitory effects on cytotoxicity were compared between original peptide and its mutant.

通过计算机模建，模拟并推测提高TNFR封闭肽生物学效应需置换的位点和氨基酸；合成原封闭肽和突变肽；受体竞争抑制结合实验比较原肽与突变肽对绿色荧光蛋白－肿瘤坏死因子融合蛋白结合TNFR的竞争抑制效应；细胞毒作用抑制实验比较原肽与突变肽对TNFR的封闭效应。

Methods We isolated and cultured the MSCs, then induced MSCs into osteoblast and checked the osteoblast by RT-PCR; the conditioned medium in different concentration was prepared containing diphosphonate or assemble flavone of drynaria rhizome; with the conditioned medium the osteoblast was cultured; then the growth curve was analyzed and the cytogene (collagenase col Ⅰ) col Ⅰ and ALP expression was detected.

使用诱导方法作用于骨髓基质干细胞，使之转化为成骨细胞；用RT-PCR检验成骨细胞；不同浓度的二磷酸盐或骨碎补总黄酮配制成条件培养基，作用于成骨细胞，并分析生长曲线，分析细胞基因碱性磷酸酶和胶原酶Ⅰ(collagenase col Ⅰ)表达情况。

Then test of film cell growth by high sugar, LPS, high sugar plus LPS was done with the result showing that FANG KIDNEY capsule can restrain the growth of film cell and composition of external base, it can be concluded that it has obvious effect in preventing the hardening of kidney small cell.

为进一步探讨糖肾胶囊的作用机制，还采用国内外经典细胞培养方法，成功地培养出肾小球系膜细胞，在此基础上进行高糖、LPS、及高糖加LPS诱导细胞膜增生，结果发现糖肾胶囊能抑制系膜细胞增生及细胞外基质胶原合成，证明其对防止肾小球硬化有明显作用。

Human/rat homology collagen type I siRNA plasmid not only direct repress collagen type I gene expression in rat inesangial cells, but also have the same inhibition action of TGF-β1 induced overexpression of collagen type Ⅰ.

本实验构建的人鼠同源Ⅰ型胶原siRNA质粒，在大鼠系膜细胞内不仅能够直接抑制Ⅰ型胶原的基因表达，而且对TGF-β1诱导的I型胶原表达增加同样具有较好的抑制作用。

The luminescence intensity of suspended immune cells (1×10〓 cells/ml) from peripheral blood, anterior kidney and spleen of grass carp, which were from two groups immunized with being injected Aeromonas hydrophilia and unimmunized respectively, were detected by means of BPCL4 Intelligentized Ultraweak Luminometer, the datum that were dealt with statistics demonstrated that luminescence intensity of the cells from all kinds of immune organs and blood were intensified after the fish were immunized in two weeks and the luminescence intensity from anterior kidney cells was the highest, mitogens had the enhancing effects. Luminol could strengthened luminescence intensity.

把未免疫的和经过嗜水产气单胞菌注射免疫的草鱼外周血、前肾、胸腺和脾脏以及它们的悬浮细胞（密度控制在1×10〓个细胞／ml左右）分别用BPCL4智能化微弱发光测量仪测量各组的发光强度，结果显示：草鱼各类免疫器官与细胞在免疫两周后发光强度增加，肾细胞的发光强度最大；有丝分裂原具有增强作用；化合物鲁米诺能提高发光强度。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究，解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢，不易形成单层的难题，建立了兔角膜上皮细胞原代培养的方法。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立；二、人胎儿角膜缘部上皮细胞增殖能力优于中央部；三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

The chip used contains 4097 genes covering oncogenes and tumor suppressor genes, the genes related to ion channels, cell cycle proteins, outer force reaction proteins, cell skeleton and movement, and apoptosis, the genes associated with DNA synthesis, repair and recombination, the genes related to DNA combining and transcription, the genes encoding cell receptors, the genes related to signaling, the genes associated with immune response, metabolism, protein synthesis, development and others.

实验所用的芯片含有4097个基因，包括原癌基因和抑癌基因、离子通道和运输蛋白基因、细胞周期蛋白基因、外压反应蛋白基因、细胞骨架蛋白和运动相关基因、细胞凋亡相关蛋白基因、DNA合成和修复和重组相关蛋白基因、DNA结合和转录和转录因子基因、细胞受体基因、免疫相关基因、细胞信号传导蛋白基因、代谢相关蛋白基因、蛋白质翻译和合成相关基因、发育相关基因、及其它基因。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。