原细胞

Methods To collect 10 specimen residual ear cartilage of microtia patients about 8-year-old and cultured in vitro, dye with toluidine blue and immunohistochemistry of collagen type II.

结果：残耳软骨细胞在体外培养时随代数增加呈异染陛的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng／mi浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

Methods Applying 1,25-_2D_3 induced osteoclast-like multinucleated cells and isolated mature rabbit osteoclasts cocultured with bone slices, the effects of icariin on the differentiation and bone resorbing activity of osteoclasts were investigated.

方法采用1，25-2D3诱导兔骨髓单核细胞形成破骨细胞样细胞以及原代分离的乳兔成熟破骨细胞与骨片共培养的方法，考察淫羊藿苷对破骨细胞的分化及骨吸收功能的影响。

Nither CDMP-1, nor cryopreservation can effect cell cycle of the chondrocytes.

结果：残耳软骨细胞在体外培养时随代数增加呈异染性的糖胺多糖减少，第1、3代Ⅱ型胶原阳性细胞比例约68％/～71％，第5代为23％、显著降低；冻存后第3代为65％，与同代数的细胞没有差别，100ng/ml浓度的CDMP-1可以将第4代细胞的阳性比例维持在66％，而且冻存和生长因子不会引起细胞周期的变化。

The ameloblasts are the key cells during odontogenesis, and they have been already cultured in vitro. The amelogenin is the extracellular matrix synthesized and secreted by the ameloblasts, and it plays a crucial role during amelogenesis.

成釉细胞是牙器官形成的关键细胞，国内外已成功离体培养出成釉细胞，成釉细胞合成和分泌的细胞外基质－釉原蛋白在釉质的形成中起着关键作用。

Brane, such as synoviocytes, macrophages, osteoclasts and TNF-αvv acts on different kinds of cell in synovial memchondrocytes, which can produce metalloproteinase,collagenase, stromelysin and so on, further induce pannus formation, joint inflammation, bone erosion and cartilage degradation.

TNF-α可作用于RA关节组织中的滑膜细胞、巨噬细胞、软骨细胞和破骨细胞，使这些细胞活化，产生金属蛋白酶、胶原酶、基膜溶解酶等，导致局部炎症反应和血管翳形成，进一步引起软骨破坏和骨侵蚀。

Methods Routine immunization of BALB/ c mice by a tansfected cell line 293T/ B7-H4 as antigens.Then the immunized spleen was fused with SP2/0,a kind of plasmocytoma cells, and screen the positive clones by FCS with the 293T/B7-H4 as a positive control while 293T/ mock as the negative control.

以高表达人B7-H4分子的基因转染细胞株293T/B7-H4为免疫原，常规免疫BALB/c小鼠，采用B淋巴细胞杂交瘤技术进行细胞融合，并以该基因转染细胞293T/B7-H4作为阳性筛选细胞，以转空质粒的对照细胞293T/Mock作为阴性对照细胞。

The mature egg cell was an inactive cell with only a few polysomes. At the early zygote stage, a large number of ribosomal precursors were produced by the nucleolus, and many polysomes appeared in the cytoplasm, which suggests a high level of metabolism. Zygote at the dormancy stage had a small nucleolus and marked decrease in ribosomes, as shown by a few polysomes, which suggests decreased metabolism. Zygotes in the prophase of mitosis and two-celled proembryo became active again in metabolism, for a prominent nucleolus, high density of ribosomes and increased number of polysomes in the cytoplasm.

结果如下：在成熟卵细胞中多聚核糖体数量不多，且细胞代谢活性较弱；初期合子内，核仁大量合成核糖体前体物质，胞质中多聚核糖体数目众多，细胞代谢活性较强；休眠期合子的核仁变小，胞质中核糖体数量急剧减少，仅有少量多聚核糖体，细胞代谢活性较弱；合子分裂前期和二细胞原胚期，核仁显著，胞质中核糖体的密度增加，出现大量多聚核糖体，细胞代谢活性较强。

Methods: The fibroblasts companying human hepatoma were primarily cultured with explant culture method, first passaged after they spread to the bottom of the culture bottle, and pured by enzyme digestion and repeated strapping the cells were identificated through observing the morphologic change using invert microscope and detecting expression of vimentin, keratin by iMmunohistochemical method.

应用组织块培养法进行人肝癌伴生成纤维细胞的原代培养，细胞铺满培养瓶底后首次传代，通过胰酶消化法和反复贴壁法进行成纤维细胞的纯化；通过倒置显微镜观察细胞形态、免疫组织化学方法检测细胞膜波形蛋白、角蛋白表达情况，对细胞进行鉴定。

In electricity plus thermal group, the pathologic alterations were slighter than model group; there was little crack on the surface of cartilage, with intact collagen fiber structure and less pyknotic chondrocytes. Although some chondrocytes shrank, there were intact cell organs in many chondrocytes even chondrocyte cluster in some regions.

疏密波+热软膜组在病理程度上的改变明显轻于模型对照组，软骨表面裂纹少，胶原纤维结构基本完整，固缩的软骨细胞少，虽可见部分退变的软骨细胞，但部分软骨细胞具有较多的细胞器，某些区域形成软骨细胞簇。

Methods:Human CD80 cDNA was transfected into B16 melanoma cells by lipofectin-mediated gene transfer before the expansion of tumor cells were tested by MTT method in vitro;C57BL/6 mice were inculated subcutaneously either mock-transfected B16 cells (B16-neo)or CD80-transfected B16 cells (B16-CD80),then the latency,survival times and tumor mass growth were investigated.The lymphocytes were examined for both proliferation indices and specific cytotoxic activity by MTT method and improved LDH-releasing method,after syngenic Mixed Lymphocyte tumor cultures.

通过脂质体介导将CD80基因导入小鼠黑色素瘤B16细胞后，利用SABC法检测CD80的表达；MTT法测定肿瘤细胞体外增殖能力；将肿瘤细胞接种至同源小鼠皮下后观察成瘤期、荷瘤小鼠存活期及肿瘤结节生长速度；在同源淋巴细胞肿瘤细胞混合培养后，通过测定淋巴细胞增殖指数和CTL杀伤活性检测肿瘤细胞的免疫原性。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。