原细胞

Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.

将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞，运用活细胞计数和碘化丙吮检测其增殖能力；采用流式细胞术检测其细胞表面标志的表达；用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化，并用Von Kossa染色进行鉴定；用地塞米松与胰岛素诱导其向脂肪细胞分化，并以油红O染色进行鉴定。

It has been used to image the living cells and localize the cell surface ultra structure under near physiological conditions by perfect atomic resolution,was also utilized in the biological process,interactions between druggery and cells,was an important instrument for the study of cell biology.

原子力显微镜，能够在接近生理条件下以具有原子级的分辨率对活细胞进行表面成像和超微结构观察，同时可以研究细胞的生物过程、细胞与药物之间和细胞之间的相互作用，成为细胞生物学研究的一种有效工具。

The atomic force microscopy.It has been used to image the living cells and localize the cell surface ultra structure under near physiological conditions by perfect atomic resolution,was also utilized in the biological process,interactions between druggery and cells,was an important instrument for the study of cell biology.

原子力显微镜，能够在接近生理条件下以具有原子级的分辨率对活细胞进行表面成像和超微结构观察，同时可以研究细胞的生物过程、细胞与药物之间和细胞之间的相互作用，成为细胞生物学研究的一种有效工具。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生；HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润，纤维细胞轻度增生，硬膜外神经根内有炎细胞浸润及炎性渗出；特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变；免疫组化染色，给药后四周及八周时，硬膜内外均有Ⅱ型胶原纤维增生；固兰染色B、C、D三组未见有明显脱髓鞘改变，与A组相比无明显异常改变；电镜观察B、C、D三组在给药后的四周及八周时，表现为硬膜外神经根内髓鞘厚薄不一，形状不规则，可见成纤维细胞，神经内膜中可见有巨噬细胞；粗大的有髓神经纤维变化明显，无髓神经纤维受累较轻；八周时电镜下D组改变较B、C两组为轻。

Methods:(1) Bone marrow stromal cells isolated from Beagle dogs were cultured in vitro for the primary culture,in basic medium consisted ofDMEM^iew horning cattleerum,etc. Parts of subculture were cultured in mineralization medium which additionally contained 50 y g/ml ascorbic acid, 10mmol/LNa- beta -Glycerophosphate and 10-8mol/L dexamethasone in order to induce cells differentiating into osteoblasts.

①抽取毕格犬髂骨骨髓体外分离培养获得骨髓基质细胞，DMEM、新生牛血清培养基进行原代培养，部分传代细胞以含10~(-8)mol/L地塞米松、50μg/ml抗坏血酸和10mmol/Lβ—甘油磷酸钠的矿化培养液诱导其向成骨细胞增殖分化，用倒置相差显微镜进行细胞形态学和细胞生长增殖观察。

The immune level tested in different time after injection with ginkgolic acids in sensitization mode showed that the expression of CD3〓 and CD4〓T lymphocytes increased, but CD8〓 was restrained, while the B lymphocytes was also increased. The results suggested that sensitivity was approved and T lymphocytes proliferation was induced, which strength cell immunity responsion, at the same time B lymphocytes was differentiated to IgE antibody-producing cells when contacted with ginkgolic acids as allergen.

大鼠经致敏方式接触银杏酸后，在不同时间的细胞免疫水平与体液免疫水平的研究结果表明，大鼠CD3〓及CD4〓T淋巴细胞的表达增加，而CD8〓T淋巴细胞的表达受到明显抑制，同时参与体液免疫的B淋巴细胞百分率呈增高趋势，提示银杏酸可能作为变应原使机体敏感性增强，诱导T细胞分裂增殖，增强细胞免疫应答，同时促进B细胞增殖分化成IgE抗体产生细胞。

To investigate the stantant changes of Ⅰ、Ⅱ、Ⅲ and PG expression of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between the differentiation and dedifferentiation of condylar chondrocytes and different compressive stresses; to investigate the stantant changes of the content of DNA of mandibular condylar chondrocytes stimulated by the different static compressive stresses, so that to make a primary understanding of the relationship between proliferentiation and apothesis of MCCs and different static compressive stresses.

观察单层培养的髁状突软骨细胞加载不同静压力后，Ⅰ、Ⅱ、Ⅲ型胶原以及蛋白多糖表达的变化，探讨不同静压力对髁状突软骨细胞的分化及去分化的即时效应；观察单层培养的髁状突软骨细胞加载不同静压力后，其增殖、凋亡及细胞周期的变化，探讨不同静压力对髁状突软骨细胞增殖及凋亡的即时效应。

Therefore, our data show that in cases when clear cell chondrosarcoma and chondroblastoma pose a diagnostic challenge, the presence of type II collagen in the extracellular tumour matrix significantly supports the diagnosis of clear cell chondrosarcoma and aids in distinguishing it from chondroblastoma.

我们的数据表明，在诊断透明细胞软骨肉瘤和成软骨细胞瘤受阻时，细胞外肿瘤基质中II型胶原的存在对于诊断透明细胞软骨肉瘤相当有帮助，并有助于和成软骨细胞瘤间的鉴别。

It is well known that retinal pigment epithelial cells are predominant proliferative cells in PVR which is an excessively wound healing response occurring after RPE cell wounding. The activated RPE cells migrate from their normal, sessile state to vitreous and onto both surfaces of the retina, in which they begin to dedifferentiate, migrate, proliferate, change phenotype and secrete ECM.

既往研究证明视网膜色素上皮(retinal pigmentepithelial，RPE)细胞是参与PVR的主要细胞，PVR是视网膜脱离后RPE细胞损伤的一种自我修复过程，是一种过度的眼内创伤修复反应；RPE细胞在正常位置上处于静止状态，而在某些病理条件下，如视网膜裂孔形成和视网膜脱离后，RPE细胞脱离原位，开始去分化、移行、增生、发生表型转化并分泌胶原等ECM，最终在视网膜前后表面和玻璃体内形成具有收缩能力的增生膜，造成牵拉性视网膜脱离。

The adherent cells from the embryo heart of E10～E12 were constituted by the difform cadiocyte with affluent kytoplasm and the slender endotheliocyte, which arranged in paralleled regularly and was seen easily to stick on the cadiocytes. CD31 antigen was expressed in the adherent of the heart, but not in the stromal cell of fetal liver from E10～E12, which showed uniform morphous with affluent endochylema.

原代心脏贴壁细胞由体积较大、胞浆丰富、呈不规则形的心肌细胞和细长梭形、胞浆较少的内皮细胞构成，容易看到心脏内皮细胞贴附在心肌细胞表面，呈均一的细长梭形排列，表达CD31抗原。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。