原细胞

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

When the concentration of NaCl was further increased to 8%, the cells of P. chrysosporium were deformed and plasma was completely vacuolated, indicative of a severe damage of P. chrysosporium. The DNA similarity was reduced to blow 67% and the rate of abnormal cells was increased to about 90%. The cells of P. chrysosporium thus essentially lost their original structure and function.

chrysosporium的结构与功能产生不良影响；NaCl浓度≥8%可对黄孢原毛平革菌产生严重损伤，细胞变形严重，空泡化，DNA相似度降至67%以下，异常细胞量约90%，表明此浓度范围可使黄孢原毛平革菌基本丧失了原有的结构与功能。

Cells were re-suspended and cultured in a humidified incubator with 5%CO_2 at 37℃.

建立巨噬细胞和死亡细胞的共培养模型在96孔板上种植RAW264.7细胞或小鼠腹腔原代巨噬细胞，分为3组，即对照组、凋亡细胞处理组和坏死细胞处理组。

Results The expression of Vimentin declined gradually during passaging process from the primary generation to the fifth generation, while the highest level of V...

结果 Vimentin在原贴壁基质细胞系由原代传至 5代的细胞中，其表达逐渐下降，而在再贴壁基质细胞传至第 3代中表达最高；Desmin、α-SmoothMuscleActin单克隆抗体和VonWillebrandFactor抗体染色呈阴性。

In this paper, male sterile line of alfalfa was used to be improved with crossing, because the seed production of crossing is low. The affecting factors of seed production of male sterile line of alfalfa and its crossing progenies that are crossed with male sterile lines of alfalfa and good alfalfa that male is fertile were analyzed. The characteristics of female reproductive organs of male sterile line and its crossing progenies are analyzed in cell morphology. The genetic difference of male sterile line and its crossing progenies are analyzed in enzyme protein. The results showed: 1、Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plant BC1-2 that is the crossing progency of male sterile line of alfalfa and Medicago varia Martin.cv.'Caoyuan No.1'is higher than male sterile lines. Rate of podding of nature crossing and fecundity of artificial crossing of the male sterile plants BC1-19、BC1-21、BC1-22、BC1-23 that are the crossing progencies of male sterile line of alfalfa and Medicago sativa L.cv.

针对苜蓿雄性不育系杂交制种产量低的问题，采用杂交法对苜蓿雄性不育系进行改良，以原苜蓿雄性不育系为轮回亲本与雄性可育优良苜蓿品种杂交，分析苜蓿雄性不育系及其杂交后代种子产量的影响因素，从细胞形态学方面观察和分析杂交后代及原不育系在雌性生殖器官上的变异特点，从酶蛋白水平对杂交后代及原不育系的遗传差异进行分析，初步研究结果如下： 1、苜蓿雄性不育系与草原一号苜蓿的杂交后代不育株BC1-2，以及苜蓿雄性不育系与X′(苜蓿雄性不育系与新疆大叶苜蓿的杂种F1代)的杂交后代不育株BC1-19、BC1-21、BC1-22、BC1-23的天然杂交结荚率和人工杂交制种的结荚率和百粒重均高于原苜蓿雄性不育系。

With the increase of cell density, soma was slenderer and slenderer and like fibroblast.② Growth curves of PDMCs: Growth latency ranged from 2 to 8 days after cell inoculation. During this period, adherence was observed gradually but not obviously amplified. Eight days later, cells entered log growth phase.

结果：①多分化潜能细胞的原代培养情况：胎盘组织消化后获得细胞中仅有少量的贴壁细胞，经两周后逐渐形成扁平单层细胞，呈漩涡状生长或成簇生长，随着细胞密度的增加，胞体变得细长，形态类似成纤维细胞。

After repeated short time digestion of pancreatic tissues with collagenase Ⅴ, cultured cells were transferred to a new culture flask.

用胶原酶反复短时间消化胰腺组织，在细胞接种后及时转板的方法可获得生长良好的高纯度胰岛原代单层细胞，适合用于实验研究。

Our results revealed that TGF-β1 at 1 ng/ml induced collagen deposition of HSC-T6 cells (145 ± 5 % of controls), and pre-exposure of cells to curcumin (1.25~10 μM) inhibited the stimulatory effect of TGF-β1 in a concentration-dependent manner. Curcumin (10 μM) significantly reduced the TGF-β1-induced collagen deposition (97 ± 7 % of controls) without cytotoxicity in the MTT assay .

我们的结果显示，1 ng/ml 浓度的TGF-β1可以有效地增加星状细胞对胶原蛋白的制造(达控制组的145 ± 5 %)，而在预先投予浓度范围为1.25~10 μM的姜黄素后可对TGF-β1引起的刺激反应产生浓度效应的抑制作用；姜黄素在浓度10 μM下可明显地抑制TGF-β1引起的胶原蛋白沉积(达控制组的97 ± 7 %)而不致产生细胞毒性。

The results demonstrated that exogenous type II collagen indeed induced the re-expression of type II collagen and aggrecan mRNAs, and glycosaminoglycan levels.

研究结果证明，细胞外间质分子之第二型胶原蛋白可刺激已去分化之软骨细胞再度分泌第二型胶原蛋白及聚葡萄糖胺。

METHODS:U937 cells were labeled with ［3H］ thymidine in the culture medium, test reagents were added into the cell suspension in 24-well plates. The counts*min-1 were counted in a liquid scintillation counter.

［3H］-TdR标记的U937细胞培养于24孔板，在高分子激肽原，激肽释放酶(kallikrein，KK)，因子Ⅻ和纤溶酶原的作用下，观察U937细胞的粘附情况。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。