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The distribution of ABH substances in humantissue cells of nonsecretors differs from those insecretors.the contents of ABH in cells of nonsecretorswere much less than those of secretors.ABH substanceswere demonstred in cells of deep part of gastric gland,Brunner gland of duodenum,acini and duct of pancreas,acini and duct of sweat gland and in endothelial cells ofblood vessels and heart.

在国内外首次用免疫透射电镜对正常人体的胃、十二指肠、横结肠的粘膜上皮和腺上皮的ABH物质进行亚细胞定位研究后发现,ABH物质分布于粘膜上皮及粘液性细胞的Golgi器、粘原颗粒和胃腺主细胞的酶原颗粒中,证实人体组织细胞的ABH物质由自身产生,而不是从血液吸附。

Xinjiang Daye'is higher than male sterile lines. 2、The pistil characters of the male sterile plants BC1-2、BC1-19、BC1-21、BC1-22、BC1-23 are improved. Hairy cells of stigma of the male sterile plants enwrap loose mastoid cells. The area of mastoid cells exterior is larger. Hairy cells of stigma of the male sterile plants are longer and abduction.Their Style is larger and wide. The cubage of the epidermal cell is larger and the arrangement of passage cells is tight.

BC1-2、BC1-19、BC1-21、BC1-22、BC1-23的雌蕊的性状得到改良,改良的杂交后代不育株的柱头毛状体细胞将中心的乳突细胞包裹较疏松,乳突细胞明显突起且裸露面积较大,毛状体细胞较原不育系MS-4的相对长而外展且分泌物较多,;改良的杂交后代不育株的花柱较原不育系的花柱宽且长,表皮细胞的体积较大,通道细胞排列较紧密。

Results Osteoclast and osteoblast around trabecula of bones showed proliferation, shi1e fatty tissues in bone marrow decreased and hematopoietic tissue well-regenerated alter the hyperbaric oxygen-treated. Also juvenal osteocyte and collagen fibril were observed in early stage by electron microscope.

结果 多数骨小梁周围可见骨母细胞增生、偶见破骨细胞增生,髓腔内脂肪细胞逐渐减少,造血组织增生;电镜发现骨小梁内早期即出现幼稚骨细胞和胶原原纤维合成。

Methods hepatocytes were digested by iv-collagenase and cultured with low-serum medium, induced by h2o2 after 12~16 h. various concentrations of calf liver extract were added to the primary cultured hepatocytes to observe the effect of (100~1 000) mg/l calf liver extract on the injured liver cell.

采用ⅳ型胶原酶消化法制备游离肝细胞悬液,以低血清进行肝细胞原代培养,12~16 h后用0.1 mmol/l的h2o2诱导肝细胞损伤,同时加以不同浓度的肝提取物,观察(100~1 000)mg/l的小牛肝提取物对肝细胞损伤的影响。

A micropipette aspiration technique was employed to investigate the adhesive properties of hepatocellular carcinoma cells to collagen type Ⅰ, based on comparison between single cells isolated from the primary culture of human fetal hepatocytes and from the culture of HCCs.

重庆大学生物工程研究院,重庆 630044本文以正常胎肝细胞的原代培养及肝癌细胞的传代培养为基础,应用微管吸吮技术在单个细胞水平上研究肝实质细胞癌细胞在胶原蛋白Ⅰ裱衬表面的粘附力学特性。

Basing on the nectary structure and polysaccharied changes, it was supposed that storing of per-nectar and decomposing of polysaccharide were conducted in nectariferous tissue. The epidermis was the main place where processing of per-nectar and synthesizing and secreting of nectar were conducted.

根据花外蜜腺的特点及多糖的变化分析,原蜜汁的贮存及多糖的降解主要在产蜜组织细胞中进行,而分泌表皮细胞则是原蜜汁的加工及蜜汁的合成与分泌的主要场所,且分泌细胞内含有浓厚的细胞质,有显著的细胞核和大量的细胞器。

These experiment focus on the chondroblast induction in MSC, the culture technology in monolayer system and triaxial system,in order to explore the ideal condition of chondroblast induction of MSC,the molecul mechanism of maintenance chondroblast phnotype and the interaction between cells and porous materials.

通过骨髓基质干细胞的原代分离、培养及成软骨诱导,观察原代细胞扩增能力及其向成软骨方向分化的潜能;寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件;u)观察经成软骨诱导的财C在PDLLA、PDLLA/壳聚糖多孔载体上三维的增殖状况,探求细胞与材料间的相互作用;门)观察经成软骨诱导的 MSC在体外三维培养系统中细胞因子的释放状况,了解维持诱导细胞软骨表型的合适条件及作用机制。

The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan. The aim are: 1 To examine the proliferation ability and potential chondroblast differentiation; 2 To find an ideal condition stimulated BMSC differentiate into chondroblast; 3 To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials; 4 To examine the release of cytokine in vitro.

目的在于:(1)通过骨髓基质干细胞的原代分离、培养及成软骨诱导,观察原代细胞扩增能力及其向成软骨方向分化的潜能;(2)寻求骨髓基质干细胞向成软骨方向分化的理想诱导条件;(3)观察经成软骨诱导的MSC在PDLLA、PDLLA/壳聚糖多孔载体上三维的增殖状况,探求细胞与材料间的相互作用;(4)观察经成软骨诱导的MSC在体外三维培养系统中细胞因子的释放状况,了解维持诱导细胞软骨表型的合适条件及作用机制。

Based on these previous work, in this research, in order to explore the the molecular mechanism of enhanced tumor cell immunogenicity treated by elemene and mytomycin C compositely, we used murine Hca-F ascitic hepatic carcinoma ( high lymphatic metastasis substrain of H22) as an experimental model to compare the immunoprotective effects on Hca-F cells as well as the mechanism of the effects caused by HSP70-peptide complex purified from untreated tumor cells, EC-TCV and BCG; We studied the mechanism of increased tumor cell membrane HSP expression treated with elemene by the techniques of RT-PCR and immunoelectron microscopy; In order to deeply elucidate the antitumor effects and the molecular mechanism of elemene in increasing tumor cell immunogenicity we analyzed and compared the effects of elemene and heat shock on the gene expressions of tumor cell using gene expression profile chip.

本论文在上述研究成果的基础上,选择小鼠肝癌H22的高淋巴道转移亚系Hca-F为模型,进一步比较了未处理肿瘤细胞来源、榄香烯复合瘤苗来源的和卡介苗来源的HSP70-肽复合物对Hca-F的免疫保护效应、效应机制,探讨榄香烯-丝裂霉素复合处理增强肿瘤细胞免疫原性的分子机制;以Hca-F和人肝癌细胞株HepG〓等为模型研究了榄香烯处理增强肿瘤细胞膜热休克蛋白表达的机制;以HepG〓为模型用表达谱基因芯片研究比较了榄香烯与热休克处理对肿瘤细胞基因表达的影响,从而进一步阐明榄香烯抗肿瘤作用及增强肿瘤细胞免疫原性的分子机制。

Since 1962, when Gross and Lapiere discovered the first MMPs-collagenase, man had discovered various of MMPs in succession, where MMP-2 has a strong degradational effect on extracellular matrix, it is secreted bymacrophage, smooth muscle cells and endothelial cells and so on.

自从 1962年 Gross和 Lapiere发现第一种基质金属蛋白酶一胶原酶以来,人类陆续发现了很多种基质金属蛋白酶,其中的MMP—2对细胞外基质具有极强的降解作用,它由巨噬细胞、平滑肌细胞以及内皮细胞等分泌,在动脉粥样硬化斑块中憎多时降解纤维帽及基底膜的胶原等基质成分,是使纤维帽变薄及加速动脉粥样硬化的重要因素之一。

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Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

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