原细胞

Water, Glycerin, Methylsilanol Mannuronate, Sodium Carboxymethyl Beta-Glucan, Sodium Hyaluronate, Palmitoyl Oligopeptide, Palmitoyl Tetrapeptide-7, Madecassoside, EGF *, FGF *, Telomerase *, Panthenol, Borago Officinalis Seed Oil, Anthemis Nobilis Flower Extract, Tocopherol, Tocopheryl Acetate, Cetyl Palmitate, Citrus Aurantium Dulcis Peel Oil, Citral, Citronellol, Limonene, Linalool, Zea Mays Starch, Butylene Glycol, PEG-40 Hydrogenated Castor Oil, Hydrolyzed Corn Starch, Hydrolyzed Corn Starch Octenylsuccinate, PEG-33, PEG-8 Dimethicone, Hydroxyethylcellulose, PEG-14, Xanthan Gum, Carbomer, Polysorbate 20, Caprylyl Glycol, Phenoxyethanol, Methylparaben.

主要有效成分是：聚羧基甲基β葡萄糖的钠盐，保湿锁水；透明质酸，高效保湿；寡胜肽；四胜肽；羟基积雪草甙，抗炎症，促进胶原蛋白的合成；表皮生长因子EGF，促进细胞新陈代谢；成纤维细胞生长因子FGF，促进胶原蛋白合成；端立酶，一种RNA与蛋白的复合体，可通过合成新的端粒而修补受损的DNA端粒，使细胞持续不停的增生，也就是利用成人干细胞转变为全新的细胞。维他命B5；琉璃苣籽油，含维他命和矿物质；洋柑橘萃取液，舒缓，抗过敏；维生素E；醋酸盐维生素E；橙皮油；水解玉米淀粉。

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原，抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加，提示它和BMP具有协同作用。

There were the radicle polarity and the plumule polarity in differentiation of the first somatic embryogenesis.

此外，体细胞胚的发生和分裂是不同步的，往往在同一张切片上既可看到单个的胚性母细胞，又可见到二细胞、四细胞以致多细胞原胚并存的多样化原胚细胞群体。

Sinense, the spores germinate inside their exospores and then divide into massive protonemata;the massive protonemata can form two kinds of protonemata of which one is clubbed protonema with papillae,and the other is caulonema composed of long and cylindrical cells ; the gametophyte archaeocytes only occur on the massive protonemata.

结果表明：中华缩叶藓的孢子在壁内萌发，随后分裂产生块状原丝体；块状原丝体上可产生两种丝状体，一种是具疣的棒状原丝体，另一种是由长圆柱状细胞组成的轴丝体；配子体原始细胞只产生于块状原丝体上。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后，胶原排列趋于一致，微血管丰富而呈开放状态，成纤维细胞的前胶原聚合体蓄积在细胞浆内；胶原总的含量减少，但Ⅲ型前胶原含量维持在一个较高水平；透明质酸含量逐渐升高，接近正常皮肤水平。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1，年龄分布自1至81岁，平均年龄33.3±19.3岁，中值年龄为34岁，29岁以下占48%(90/194)，其中99例单独或手术后辅以放射腺治疗，分析同种脑瘤的预后因素，其中多形神经胶母细胞瘤有手术后放射线治疗或女性，多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射，神经管胚细胞瘤，间胶质母细胞瘤，及室管膜瘤原发肿瘤剂量4500雷得以上，室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳，且统计上有显著的差异。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Using New Zealand rabbit ears cartilage makes chondrocyte suspension, Fibrinogen was mixed with D-hank's makes a final fibrinogen concentration of 50mg/ml mixture solution, then chondrocytes resuspend with fibrinogen solution, chondrocytes-seeded fibrinogen was mixed with thrombin (25U/ml in 40mM calcium choride) to make chondrocytes/fibrin glue polymer. Pluronic F-127 was mixed with D-hank's makes a final Pluronic F-127 concentration of 400mg/ml mixture solution, then chondrocytes resuspend with Pluronic F-127 solution to make chondrocytes/Pluronic F-127 polymer. The chondrocyte concentrations was 10 million chondrocyte/ml of polymer.

为确定纤维蛋白凝胶与Pluronic F-127在注射方式形成组织工程化软骨过程中的优劣，我们进行了如下实验：应用新西兰大白兔耳软骨获取软骨细胞并体外培养，纤维蛋白原应用D-hank's液配制为50mg/ml，然后以纤维蛋白原溶液重悬软骨细胞，再与40mM的氯化钙配制的25U/ml凝血酶混合形成软骨细胞—纤维蛋白凝胶复合物；Pluronic F-127应用D-hank's液配制为400mg/ml，同样用PluronicF-127溶液重悬软骨细胞而形成软骨细胞—Pluronic F-127凝胶复合物。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Due to potential instability of the prophages carrying the stx gene, in this study, the prophage of O157:H7 86-24 strain was cured with serial passages of bacteria, confirmed by PCR and DNA sequencing. The cultural supernatant of O157 prophage curing mutant was inoculated into Vero cells, indicating that the prophage curing mutant of O157 lost the toxigenicity to Vero cells.

由于原噬菌体本身具有潜在的不稳定性，本研究以O157：H7 86-24为始发菌株，将其进行传代培养，筛选原噬菌体消除菌株，对原噬菌体在O157基因组中的整合位点进行分析，并用Vero细胞对原噬菌体消除菌株的细胞毒性进行鉴定。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。