原细胞

The results show that, with the maturity of the gonad, the undifferentiated germ cell divided and differentiated to smaller oogonium or spermatogonia, and the oogonium or spermatogonia transformed to oocyte and sperm undergoing a series of maturation process.

摘 要 通过观察栉江珧的未分化生殖细胞，精原细胞和卵原细胞，精母细胞和卵母细胞，精子和卵子的形态结构与分布状况及核仁在卵成熟过程中的变化，对栉江珧生殖细胞发生及成熟过程的有关问题进行了探讨。

According to the size and shape of oocyte, the morphology of nucleolus, the growth of yolk and the structure of follicle, oogenesis of H. d. supertexta can be divided into three stages as follows: oogonium, previtellogenic oocyte and vitellogenic oocyte. The ovary wall is composed of tunica adventitia and germinal epithelium which will produce oogonia and follicle cells. The follicle is the structure unit of ovary.

根据卵细胞的大小、形状，核仁的形态，卵黄颗粒的积累情况，滤泡的结构等，将九孔鲍卵子的发生分为卵原细胞、卵黄发生前的卵母细胞和卵黄发生期的卵母细胞3个时期；卵巢壁由外膜及内生殖上皮构成，生殖上皮分化产生卵原细胞和滤泡细胞；卵巢的结构单位是滤泡。

Conclu sion:(1)Surface densities of PCNApositive cells reached their peaks on 14th day after birth,but the number of apoptotic cells reached its peaks on 21st day p ostnatal,so the apoptotic peak was late the proliferating peak for a week,and related to the particular period in cycle of seminiferous epithelium;(2)The spe rmatogium were found move from center to the periphery of the seminiferous tubule and arrange disorder to order;(3)The spermatogonium remained replicating activ ity of DNA during the developing stage.

生精细胞凋亡的高峰要滞后其增殖峰1周左右，而且与其所处生精周期的特定阶段有关；（2）精原细胞在胚胎发育过程中向曲细精管周边迁移，其排列由无序到有序；（3）在生后各发育阶段中，精原细胞始终保持DNA复制的能力。

The females incubated at 33 , their ovarium has powerful cortex, the germinal epithlium produce oocyte, its medulla degenerate into ovarian cavity. During sexual differentiation, mitochondria and smooth endoplasmic reticulum in cells of ovary increase in quantity, they disintegrate after sexual differentiation. There are a lot of big grana in cells of ovary before sexual differentiation, in the course of sexual differentiation the quantity of the big grana decrease, while small grana increase and peakafter sexual differentiation. The males incubation at 26 , cortices of their testes degenerate into a thin albuginea. Medulla are formed by testicular cords in which scatter spermatogonium.

33℃下孵化的小龟卵巢皮质发达，由原始生殖细胞产生卵原细胞，髓质退化成为卵巢腔，分化时卵巢细胞的超微结构显示线粒体和内质网的数量大大增加，它们在分化结束后解体，分化前细胞内有较多的大颗粒，在分化中大颗粒减少，小颗粒增多，在分化后小颗粒达到最多。26℃下孵化的小龟精巢皮质退化成为一层薄的白膜，髓质有许多睾丸管构成，管腔内有散落排列的精原细胞。

The first 0.1%collagenaseⅣ 0.1% hyaluronidase for 20 min and than 0.25% trypsin 0.04% EDTA 5μg/mL DNaseⅠfor 5 min,its enough to obtain germ cells and have optimal cell viability. 8.4±2.6×106 cells per gram testis were isolated and there was(19.7±5.3)% of PGP9.5 spermatogonium and (23.8±3.6)% of c-kit spermatogonium.

采用两步酶消化法，0.1%胶原酶和0.1%透明质酸酶消化20 min，0.25%胰酶 0.04% EDTA 5μg/mL DNaseⅠ处理5 min，就可以使睾丸生殖细胞充分分离处出来，每克睾丸组织克获得8.4±2.6×106个细胞，其中PGP9.5 精原细胞占(19.7±5.3)%，c-kit 精原细胞占总数的(23.8±3.6)%。

Under normal temperature, anthers of not fertility restoration stopped developing in the stage of archesporical cell; 1-2 comers of a few anther could differentiate and form archesporical cell, sporogenous cells and microspore mother cell, but microspore mother cell disintegrated on the first meiotic division.

常温下，未发生育性恢复的花药在孢原细胞期就停止发育；少数花药1~2个角隅处可分化形成孢原细胞，造孢细胞，花粉母细胞，但花粉母细胞在减数分裂的第一次分裂时解体。

They can reach 72.04% and 84.5% separately(P.05). 2. The methods such as Percoll uncontinuous density gradient centrifugation, isolation according to the different adhesiveness can be used to isolate and purify rabbit SSC effectively. Spermatogonia mainly distribute between the gradient 27% to 35%(density between 1.0410g/ml to 1.0508g/ml).

Percoll 不连续密度梯度离心和差异粘附等方法可以有效的对兔精原细胞进行分离纯化，其中精原细胞主要分布于密度梯度为27%-35%(密度 1.0410g/ml~1.0508g/ml)的带间，此带中精原细胞的纯度平均达到 60.42%(P.01)。

Strong expression of nanos 1 mRNA was detected in spermatogonia and relatively weaker signal was found in primary spermatocytes but no nanos 1 mRNA expression was observed in spermatids. The results suggested that nanos 1 may play an important role in maintenance and functioning of the germline stem cell—oogonia and spermatogonia.

在斑马鱼精子发生中， nanos1 mRNA可在精原细胞和初级精母细胞中检测到。nanos1 mRNA的阳性信号在精原细胞中极为强烈，在初级精母细胞中较为微弱，而精子细胞中没有阳性信号。

The results were as follows: nanos 1 mRNA uniformly dispersed throughout the cytoplasm of oocytes at all stages. Strong expression of nanos1 mRNA was observed in oogonia and stageⅠ、Ⅱ oocytes, but the signal became weaker in later stage oocytes.

结果表明：在斑马鱼卵子发生中，nanos1 mRNA均匀分布于卵原细胞和各时期卵母细胞的胞质中；在卵原细胞和Ⅰ、Ⅱ期卵母细胞中，nanos1 mRNA的杂交信号十分强烈，而较晚期卵母细胞中信号明显减弱。

Also, the intensity of immuno-positive reactions to them enhance with the development of the gonads.

免疫组织化学法也显示卵原细胞、卵母细胞、精原细胞、精母细胞和精子细胞存在睾酮和17β-雌二醇的免疫阳性物质，而且睾酮和17β-雌二醇的免疫反应强度也是随着性腺发育成熟而增强。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。