原纤维的

Objective To investigate the proliferation of porcine meniscus fibrochondrocytes and the expression of collagen under the condition of monolayer culture to fabricate autologous tissue-engineered menisci.

目的 研究体外单层培养条件下猪半月板纤维软骨细胞增殖和胶原的表达，为构建自体组织工程化半月板奠定基础。

This method was used to investigate the arrangement of CMF in the inner wall of cell cavity of bamboo fibers.

该研究证实，利用原子力显微镜可以实现对竹纤维微纤丝取向的高分辩观察，并且样品制备远较透射电镜简单，可操作性强。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用组织块培养法获得原代CAFs和正常粘膜成纤维细胞，0.25%胰蛋白酶消化传代和纯化细胞；通过形态学观察和免疫细胞化学染色对口腔CAFs和NMFs进行鉴定：采用半定量RT-PCR检测口腔CAFs和NMFs 4种基因的表达。

Methods Hepatic cells were obtained from fetal rats by means of mechanical separation and hanging-drop culture, and after two days of primary culture, the medium was changed for further cell culture in the presence of 20% fetal bovine serum (containing glucose 25ml/L, mycillin 100 U/ml, pH 7.6), 10 mmol/L nicotinamide, 1 mg/L insulin, affix N2, basic fibroblast growth factor, stem cell factor, epidermal growth factor and leukemia inhibitory factor.

采用机械分离法结合悬滴培养法获得胎肝干细胞，原代培养2d后改变培养基，添加含20%胎牛血清的DMEM（含25ml/L葡萄糖，100U/ml青链霉素，pH值7.6），添加烟酰胺10mmol/L，胰岛素1mg/L，N2添加剂，碱性成纤维细胞生长因子、表皮生长因子、干细胞因子、白血病抑制因子各20μg/L诱导分化为nestin阳性细胞。

"Second, both solid tumors and ascular anomalies (e.g., hemangiomas, port-wine stains) are known to hae increased expression of angiogenic proteins, including ascular endothelial growth factor and basic fibroblast growth factor."

&第二，已知实体瘤和脉管异常（例如血管瘤、葡萄酒色痣）存在血管原的蛋白表达增加，包括血管内皮生长因子和碱性成纤维细胞生长因子。&

In the exudates from wounds, IL-1 was detected. IL-1 is capable of promoting proliferation of fibroblasts and excretion of collagenase, which induces inflammation and fever and promote wound healing. Inflammation is a defense reaction of the body.

同时，在局部创伤渗出液中测出含有IL-1，而IL-1可促进纤维细胞增殖及促进分泌胶原酶，引起炎症和发热，促进创面愈合，而炎症反应被认为是机体的防御反应，因此MEBO具有抗感染、止痛、促进创面愈合和减轻瘢痕形成等作用。

The methods of sticking tissues piece to culture primary hFCECs and mouse 3T3 fibroblast conditioned medium to culture passage hFCECs were established;2.The Proliferative capacity of corneal limbus hFCECs is better than central hFCECs.3.3T3 conditioned medium could markedly promote the proliferation of epithelial cells

结论一、组织块贴壁法原代培养人胎儿角膜上皮细胞以及小鼠3T3成纤维细胞条件培养液传代培养方法基本建立；二、人胎儿角膜缘部上皮细胞增殖能力优于中央部；三、3T3细胞条件培养液能促进角膜上皮细胞的增殖。

Fibroblasts are responsible for the production of collagen.

成纤维细胞，负责生产的胶原蛋白。

OBJECTIVE: To investigate the multi-differentiation capacity of human lung fibroblasts.

目的：观察原代培养的人肺成纤维母体细胞外分化特性。

RG has an inhibitory effect on the formation of liver fibrosis and cirrhosis. Its mechanism may be related to the improvement of hepatic function, decrease of serum TGF β1, collagen Ⅰ and Ⅲ and Hyp levels.

结论］软肝颗粒能改善肝组织病理变化，恢复肝功能，降低血清TGFβ1和Ⅰ、Ⅲ型胶原水平及Hyp含量，抑制肝纤维化、肝硬化的形成。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。