原纤维的

In addition, after stably expression of bFGF, the biological characteristics of BMSCs and bone marrow-derived osteoblasts were analyzed in the fields of morphology, proliferation, cell cycle, alkaline phosphatase, typeⅠ collagen and so on.

从形态、增殖特性和细胞周期、碱性磷酸酶、Ⅰ型胶原等方面分析稳定表达碱性成纤维细胞生长因子的骨髓间充质干细胞和骨髓源性成骨细胞生物学特性。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测：将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心，取上清液，按Blanckaert法测定胶原酶，所得数据经统计学处理差异无显著性。

Immunohistochemical technique was carried out to examine the distribution of collagen type IV and fibronectin in decidua of both groups.

采用免疫组织化学法测定早孕蜕膜组织IV型胶原(对照组15例，药物组20例)和纤维粘连蛋白(对照组25例，药物组30例)的分布状况。

The dermis is comprised primarily of connective tissue, which is made mostly of collagen and elastin fibers.

真皮是由主要的结缔组织构成，它主要由胶原蛋白和弹性蛋白纤维构成。

BACKGROUND: It is generally accepted that fibrous collagen protein is an ideal vector, limiting by easy fluxion, rapid degradation, and doubtable permeability.

背景：纤维胶原蛋白被公认是非常理想的载体，但其胶性易流动，降解较快，且通透性有待置疑。

This occurs in the skin's dermis, as a result of collagen and elastin fibers becoming hard, thick, and then binding together.

这是发生在皮肤的真皮层，由于胶原蛋白和弹力纤维越来越硬，厚而然后结合在一起。

The thesis consists of six chapters. In the first, the technologies of atomic force microscopy and of the measurement elasticity of biomolecules were introduced. In the second, the validity of VSPFM was confirmed by lift mode atomic force microscopy. In this chapter, the height of DNA was measured by lift mode atomic force microscopy, which demonstrated that the method of height measurement of biomolecules by VSPFM was correct and established the foundation of the method of measurement elasticity of biomolecules by vibrating mode scanning polarization force microscopy. In the third chapter, detailed work has been illustrated on the foundation of the method of measurement elasticity of biomolecules by VSPFM. And the compressive elasticity of DNA was measured. In fourth and fifth chapters, the method was applied in the measurement elasticity of proteins. Two proteins elasticity, fibre-like protein α-synuclein and global protein IgG, were measured by VSPFM, through which the method wound its way to the application of biomolecules. In last chapter, the final part of the thesis was a summary. A conclusion of the thesis and a self-comment on my work as a PhD candidate have been made, and expectation about the further works has been addressed.

本论文共分为六章，第一章，引言部分主要介绍了原子力显微镜技术及生物大分子弹性测量技术；第二章主要是VSPFM方法的正确性论证，介绍抬高模式原理，利用抬高模式原子力显微镜对DNA的高度进行测量，论证振动模式扫描极化力显微镜测量生物大分子的高度的正确性以及准确性，从而为振动模式扫描极化力显微镜测量生物大分子的弹性方法的建立奠定基础；第三章以脱氧核糖核酸为例详细介绍了振动模式极化力显微镜测量生物大分子弹性的方法的建立，对DNA的压弹性进行了初步的测量和分析；第四章和第五章介绍了振动模式扫描极化力显微镜在蛋白质弹性测量中的应用：α-synuclein和IgG分别是纤维状蛋白和球状颗粒蛋白，通过振动模式扫描极化力显微镜测量这两种蛋白质的弹性，摸索振动模式扫描极化力显微镜在蛋白质弹性测量中的应用；第六章对全文进行了总结，在对论文的工作进行归纳和自我评价之后，还对进一步的工作进行了展望。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明：①在适宜的浓度体外培养条件下，NSCs能形成典型的神经干细胞克隆球，Vimentin免疫荧光染色阳性，单靠丝裂原刺激或NSCs自我调节和分化诱导，不会产生典型的终末分化的成熟神经细胞；②海马源性的NSCs维持干细胞特性的时间更长，生物学特性更优；③传至第2～3代的NSCs尚未分化时移植最佳；④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞；⑤各种子代克隆球和细胞存在广泛的突触联系；⑥NSCs与OECs联合培养时，不需丝裂原刺激即能形成克隆球，获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞；⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化，但并不出现终末分化；⑧本研究培养条件不利于成纤维细胞、O4生长。

In carcinomatous mucosa, immunoreaction of the carinomatous cell nest was negative, or only remnants of positively were seen at the site of the former membrane by collagen type Ⅳ.

结果 正常组织的基底膜呈现很强的Ⅳ型胶原抗体阳性反应，在基底膜形成较粗的环形染色带；Ⅲ型胶原抗体阳性反应在细胞外间质呈连续的细丝纤维样。

AFGF mitogenic stimulation also results in a decrease in cellular volume and inhibition of fibroblast-mediated contraction of the collagen gel.

aFGF mitogenic激励另外在在胶原质凝胶体的停止纤维原细胞紧缩的细胞的卷和禁止的减少的结果。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。