原纤维的

Among them, in tumour of glue mother cell, visible head pledges fasciculus is interrupted in vain, its stop fiber to be able to be through corpus callosum; in meningeal tumour, visible tumour oppresses the head that cause pledges the fiber shift and remaining, fasciculus; that is departed is in in vain a tumour of cell of metamorphic star form is medium, DTT expression sends place head to pledge fasciculus is interrupted in vain formerly for tumor and tumor countershaft dash forward infiltration.

其中，在胶母细胞瘤中，可见脑白质纤维束中断，其终止纤维可通过胼胝体；在脑膜瘤中，可见肿瘤压迫引起的脑白质纤维移位以及残存、被分离的纤维束；在间变性星形细胞瘤中，DTT表现为肿瘤原发部位脑白质纤维束中断以及肿瘤对轴突的浸润。

In this article, we used automotive immunohistochemical stainer to check the expressions of HBsAg and HCV antigens in 100 cases of HCC and their adjacent tissues, mean while, we checked their histological activity index and hepatic fibrosis degree. We also used radioimmunoassay to detect serum levels of 4 serum fibrosis markers and serum AFP levels in these cases. Immunofluorescence labeling and flow cytometry were used to check the T-cell subpopulations and the activity of NK cells of these 100 cases. Then the correlations among these factors were studied.

本文采用全自动免疫组化仪对100例肝癌组织及癌旁组织中的HBsAg、HCV抗原表达进行了标记，同时对其进行肝组织活动指数、纤维化分期；同时采用放射免疫测定方法检测同期血清透明质酸、Ⅲ型前胶原蛋白、层粘连蛋白和Ⅳ型胶原蛋白等四项血清肝纤维化标志物和血清甲胎蛋白水平；采用免疫荧光标记技术和流式细胞仪对T细胞亚群及NK细胞活性测定，对各项指标的相关性进行系列对比研究。

Results: The staining of VG, HE show that the subepidermal thickness were reduced , the arrange of collagen tended to uni...

结果：兔耳腹侧面增生性瘢痕局部注射苦参碱后，按时间段取材；VG和HE染色显示真皮层明显变薄，成纤维细胞数量明显减少，胶原排列趋于一致；12周后，对照组苦参碱组的增生指数、成纤维细胞数密度、胶原密度分别为3.25±0.89/2.15±0.12，4517.5±879.6/3551.2±234.1，39.89±14.12/28.75±10.87。

Explore JIAWEISANRENTANG Decoction on liver fibrosis in rat tissue plasminogen activator inhibitor,tissue-type plasminogen activator impact.

探讨加味三仁汤对肝纤维化大鼠组织纤维酶原激活剂抑制剂、组织型纤维酶原激活剂的影响。

Methods:(1) ADM was produced from swine skins treated with trypsin followed by Triton X-100.(2) type I collagen of mouse tail was extracted by 0.5mol/L acetoacetic acid.(3) Two kinds of ADM were got by being soaked with 0.02% glutaraldehyde for 5~10 min or being soaked with mouse type I collagen for 24h, then preserved at 4℃.

(1)应用胰蛋白酶消化-去污剂法制备ADM；(2)用0.5mol/L乙酸溶液提取制备I型鼠尾胶原；(3)用0.02%戊二醛溶液浸泡ADM5-10min，制成交联型ADM；另一部分ADM用质量分数0.25%的I型鼠尾胶原浸泡24h，制成胶原包埋型ADM，冰冻保存；(4)用酶消化法培养原代成纤维细胞，取第三代增殖期细胞，将其调整至浓度为2×105/ml的成纤维细胞悬液。

Results After used the SGS , the arrange of collagen tend to unifying , micrangiums are rich and in opening condition, the polymeride of pre-collagen accumulate in cytochylema in fibroblast, the total content of collagen is decreased, but the content of hPcⅢ is kept in a high level, the content of HA is increased gradually,which closes to the normal skin level.

结果 应用硅凝胶膜后，胶原排列趋于一致，微血管丰富而呈开放状态，成纤维细胞的前胶原聚合体蓄积在细胞浆内；胶原总的含量减少，但Ⅲ型前胶原含量维持在一个较高水平；透明质酸含量逐渐升高，接近正常皮肤水平。

Results Histologically, the tumor was composed of mildly cellular benign-appearing spindle-shaped cells and bands of hyalinized collagen in varying proportions from area to area, and a typical patternless archite...

结果镜下见肿瘤组织由不同比例的纤维母细胞和胶原构成，纤维母细胞分化良好，形态多样，散乱穿梭于胶原蛋白束之间或环绕在玻璃样变的血管周围，伴有局灶性致密区域；胶原成分发生玻璃样变，局部呈轮辐状或瘢痕状排列。

This pattern is characterized by the combination of pro-MMP-2 and membrane type 1 (MT1)-MMP expression, which drie pericellular generation of actie MMP-2 and local degradation of normal lier matrix. In addition there is a marked increase in expression of TIMP-1 leading to a more global inhibition of degradation of fibrillar lier collagens by interstitial collagenases (MMP-1/MMP-13). These pathways play a significant role in the progression of lier fibrosis.

其降解肝正常基质，同时抑制引起肝纤维化的纤维胶原降解，这种情况的特点是前明胶酶A合成和膜型1－MMP表达，使得小叶内细胞激活MMP-2和降解当处的正常肝基质，此外血浆1型组织基质金属蛋白酶抑制剂（TIMP-1）明显升高引起广泛的抑制间隙胶原酶(MMP-1/MMP-13)对纤维肝脏胶原的降解。

To evaluate the role of collagen Ⅰ,Ⅲ and Ⅳ in the formation of the pericystic layer of hepatic hydatid cyst The expression of collagen Ⅰ,Ⅲ and Ⅳ in pericystic layers of hepatic hydatid cysts from 40 patients was observed using immunohistochemical methods.

探讨Ⅰ、Ⅲ和Ⅳ型胶原在形成肝包虫囊肿周围人体纤维囊壁中的作用及其临床意义。采用免疫组织化学方法检测Ⅰ、Ⅲ和Ⅳ型胶原在40例肝包虫囊肿周围纤维囊壁中表达。Ⅰ、Ⅲ和Ⅳ型胶原在肝包虫囊肿周围纤维囊壁中出现特异性分层表达。

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。