原生质膜

Malondialdehyde accumulated in protoplast of mesophyll cell of Arachis hypagaea during the cell wall digestion in enzyme solution.

植物原生质体分离期间，所用细胞壁降解酶和高渗介质对细胞生理影响甚大，其不良因素首先反应在膜的变化上。

When La3 in high concentration,in spite of in short time treatment,the ratio of electrolyte exosmosis is also increased. This probabily indicated high concentration La3 can destroyed the membrance ofroot.

高浓度的镧(c＞500 ug／ml)时，即使短时间的处理，也会使电解质外渗率提高，这可能和高浓度的镧使根细胞原生质膜破坏所致。

In this paper, leaf epidermis of Arabidopsis thaliana were used as main material, guard cell protoplasts with high activity were isolated, the relation between H~+-ATPase and calcium channel in plasma membrane of guard cells was investigated with whole cell patch clamping.

本论文以WS生态型拟南芥叶片表皮为实验材料，成功分离出高活性的保卫细胞原生质体，利用膜片钳技术对保卫细胞信号转导过程中质膜质子泵与质膜钙通道之间是否存在一定的关系进行了研究。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The ligand encoded by SCR gene is a small pollen coat protein present on the surface of pollen grains. The receptor localized to the plasma membrane of the stigmatic papillae on the surface of the pistil is encoded by SRK gene.

配体是位于花粉粒表面的一个小的胞被蛋白，由SCR基因编码；受体是位于柱头乳突细胞原生质膜上的跨膜的蛋白质激酶，由SRK基因编码。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Lomasome An infolding of the plasma membrane found particularly in fungal hyphae and spores, and also in some algae and higher plants.

质膜外泡：原生质膜的内折，尤其存在于真菌的菌丝和孢子中，在某些藻类和高等植物中也存在。

In the neutral pH of the plasma membrane, apo-transferrin dissociates from the receptor.

在原生质膜中性pH值条件下，APO-转铁蛋白与受体解离。

One of the two silicified halves of the cell wall of a diatom.

分离亚细胞组成的第一步总是使细胞壁和原生质膜破碎。

It wasconfirmed that the damage of root cell membrane is one ofthe mechanisms for Al phytotoxicity.

证实了铝离子的毒害机理之一是它损伤了原生质膜。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。