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The synthesis and secretion of a-amylases was induced by gibberellins in the protoplasts of cereal aleurone layer cells.

用GAs处理禾谷类糊粉细胞原生质体后,可以诱导α-淀粉酶的合成与分泌。

The study aimed to establish the protoplast transformation system in Alternaria tenuissima.

[目的]建立极细链格孢菌的原生质体遗传转化体系。

Malondialdehyde accumulated in protoplast of mesophyll cell of Arachis hypagaea during the cell wall digestion in enzyme solution.

植物原生质体分离期间,所用细胞壁降解酶和高渗介质对细胞生理影响甚大,其不良因素首先反应在膜的变化上。

In addition to natural water contains large amounts of solid impurities, there are micro-organisms, such as the barnacle seawater, algae, bacteria, viruses and protoplasm and so on.

天然水中除含有大量固体杂质外,还有微生物,如海水中的藤壶、海藻、细菌、病毒及原生质等。

This push-in style bioplast replacement nose ring can be used with any 18 gauge push-fit nose ring..

这推入式风格的原生质体更换鼻声,可用于任何18号推适合鼻环。。

This is an unfortunate name, because they are not cilia but long protoplasmic extensions.

这个名称不太恰当,因为它们不是纤毛,而是长的原生质突。

Three 3 biofilter models constructed with the functional strains of Xhhh and Fhhh and the native strain NJ to degrade BaP existing in the source of dinking water from Yangtze River was conducted in this research.

以跨界原生质体融合菌株Fhhh和Xhhh,及土著细菌NJ为出发菌株,建立3个生物滤池模型,探索研究深度降解长江饮用水源中3、4-苯并芘的环境生物技术。

The flocculence of fusant strain GR5 was stronger with 2.7mL of burns value.

报道了啤酒酵母原生质体融合株GR5的中试结果。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

Honghezi,Dongbi,Shieryuelongyan,etc.The results showed that the method of Calcium-alginate -embedded suspended culture shaking at50r/min was suitable for longan proto plast culture;and the optimal regeneration,with up to5%of microcolony formation,occurred when protoplasts from suspensions were cultured ...

研究结果表明,海藻酸钙包埋悬浮振荡培养(50r/min)是龙眼悬浮细胞原生质体培养的适宜培养方式,在含有多种生长调节剂的改良MS培养基(MP6)上,再生小克隆的形成频率可高达5%;采用龙眼胚性愈伤组织体胚诱导、成熟和萌发的优化方案,原生质体再生小克隆分化子叶形胚状体的频率达100%,萌发植株的频率一般大于45%。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。