原生质的

According to the results of morphological, cytological and molecular identification, it was proved that cybrids were obtained from 7-0-8-4+66-3 fusion combination, and the petaloid cytoplasmic male st

论文对影响胡萝卜原生质体非对称融合的若干因子、组培茵的先期抽墓、利用非对称融合转移胡萝卜瓣化型雄性不育性的可行性、本研究存在的问题及改进方案以及非对称原生质体融合在胡萝卜资源创新中的应用价值等进行了讨论。

In order to improve the ability of degrading anthracene and the survival of exogenous bacteria An, protoplast electrofusion was performed between An which can degrade anthracene effectively and Tu which has high survival ability, and then a fusant with specific properties was selected from the regenerated colonies.

为了提高外源蒽降解菌An降解蒽的能力和存活能力，采用原生质体电融合技术，在蒽降解菌An和土生优势菌Tu之间实行了原生质体电融合，并从再生的融合子F中筛选到具有特定性状的后代菌株。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种，筛选高产菌株在原生质体条件下，对赤霉素产生菌m978的原生质体进行菌种选育；使用单因子诱变方法，LiCl处理的效果优于DES诱变和UV诱变；复合诱变比单因子诱变更容易得到高产突变株，且获得的高产突变株稳定性更优。

In order to use the techniques of tissue and protoplasts culture for the plant breeding, it i prerequisite to understand the morphogenic potential of different varifies in tissue culture, and set up an efficient protoplast system capable of sustained divisi〓n and plan〓 r〓generation.

为了将组织培养和原生质体培养技术用于育种目的，必须了解不同品种的红豆草在组织培养中的再生能力和建立一个高效率的原生质体培养与再生的实验系统。

Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.

采用液体浅层培养方法，对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao＇s培养基中2-6天分裂，2周内形成细胞团，19天时的分裂率和克隆率分别为71.41-76.19％、64.78-67.03％。

As compared with the tissue of young leaf, the contents of RNA, DNA and total nucleic acid in freshly isolated protoplasts from sugarcane Saccharum officinarum L.

与幼叶组织相比，酶法新鲜分离的甘蔗和烟草幼叶原生质体内的RNA、DNA及总核酸含量均降低。其原因可能是刚游离的原生质体内酸性和碱性RNA酶与DNA酶等活性提高所致。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中，源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落，而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落，暗示担孢子中的两个核具有相同的交配型。

Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.

确定了双亲原生质体形成与再生的最佳条件：菌株F46、SC1采用二次菌丝培养，即在蔗糖含量20%菌丝培养液里，25℃条件下150 rpm培养48 h；然后按15%的接种量转接到蔗糖含量30%，甘氨酸含量分别为1%、1.5%的菌丝培养液中，同样条件下，继续培养48 h；在34℃条件下，菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1，菌株SC1用10 mg·mL-1溶菌酶，分别酶解3 h、2.5 h，不仅有大量的原生质体形成，且再生率也高。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种，提高多杀菌素产量，研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响，并考察了不同再生培养基和渗透压稳定剂对其再生的影响，确定了该菌株原生质体制备和再生的最佳条件。

The level of nucleic acid decreased and activities of both nucleases increased are markedly greater in sugarcane leaf protopl...

在细胞壁降解产物的作用下，除了甘蔗原生质体内的RNA酶活性略被促进外，其DNA酶和烟草叶原生质体内的核酸酶均不受影响

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。