原生质的

In addition, the application of bacteria protoplast fusion technique in genetic breeding was reviewed in the article and the development of this technique was looked forward to.

介绍了细菌原生质体融合技术在遗传育种中的应用，并展望了细菌原生质体融合技术的发展前景。

They are usually associated with specialized parenchyma cells called COMPANION CELLS, whose protoplasts are connected with the protoplasm of the sieve tube by plasmodesmata.

筛管常与特殊薄壁细胞相联系，这种细胞被称为伴胞，其原生质和筛管内的原生质有丰富的胞间连丝相通。

The result showed that the rate of production and their frequency of protoplasts division were strongly dependent on the state of the calli which were isolated by enzymatic method.

研究结果表明，酶法分离原生质体的产率和分裂频率明显取决于用于制备原生质体的愈伤组织的状态。

And on the basis of this protocol,protoplast electrofusionof cabbage with radish was studied to find out the condi-tions and parameters affecting electrofusion.

本研究通过对甘蓝子叶、萝卜子叶和下胚轴原生质培养的研究，建立了甘蓝与萝卜原生质体培养再生植株体系。

Plant protoplasts from several species of plant were fused successfully by electrofusion method, and a good procedure for electrofusion of plant protoplasts in wideinterval chamber was established according to the results.

在植物原生质体的电融合研究中，着重进行了甘蓝与大白菜原生质体的杂交融合和异核体培养，并建立了实用的电融合方法和操作程序

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

Cell wall formation and division of protoplasts derived form E. radiata suspension cells were observed. However, division did not progress beyond this stage and the protoplasts died after one month.

来自放射桉悬浮细胞之原生质体培养，可观测到原生质体分裂及细胞壁的形成，唯经培养1个月后，并未再进一步的发展而渐死亡。

On the basis of protoplast culture, we performed protoplast fusion between Astragalus melilotoides Pall, and Zygophyllum xanthoxylum Maxim using a modified PEG high-pH, high-Ca2 method. The protoplasts of A. melilotoides were pretreated with Rhodamine-6G and those of Z.

在成功培养原生质体的基础上，用改进的PEG-高pH高钙法诱导草木樨状黄芪和木本霸王原生质体融合，得到了科间体细胞杂种融合细胞。

The results show that the suitable conditions are enzyme concentration 5mg/mL, my-celium age 24h, zymolytic temperature 37 ℃, time 1h. The protoplast formation rate is 100%, The regeneration rate is 64.6%.

结果表明：出芽短梗霉原生质体制备所需的最佳条件是酶浓度5 mg/mL、菌龄24 h、酶解温度37℃、酶解时间1 h，在此条件下原生质体形成率达100%，再生率为64.6%。

The results showed that about 3.18 x l0~7/ml of protoplasts were formed when the mycelia were incubated for 1 hour at 28 °C in TSB medium containing 2% glycin, and treated with 2 mg/ml lysozyme.

对东方拟无枝酸菌原生质体制备、再生及质粒DNA转化等条件进行了探索，考察了培养基组分、甘氨酸浓度、溶菌酶浓度等因素对原生质体的制备及其再生的影响。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。