原生质的

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

The bacteriostatic effects, action mechanisms and antimicrobial spectrum of the strains were tested. After 5 days of culture, a bacteriostatic belt of 6.9-9.67 mm in width was observed on the culture plate, and the inhibitory activity maintained for 10 d. Prefiltration fermentation of the strains P1 and P5 could significantly inhibit conidial germination of the pathogen, with inhibitive rate ranging from 97.51% to 98.12%. The action might be realized by inducing hyphal deformity, bioplasm concentration, conidial germination inhibition and distorting germ tubes of conidia.

通过对其抑菌效果、机制和抗真菌谱进行测定，结果表明，P1、P5和葡萄灰霉病菌对峙培养5 d后，均产生明显的抑菌带，抑菌带宽度为6.90～9.67 mm，培养10 d后，仍然保持稳定的抑菌效果；P1、P5的发酵原液对葡萄灰霉病菌孢子萌发具有显著的抑制作用，抑制率为97.51%～98.12%；P1、P5对病菌的作用机制表现为使病菌菌丝畸形、原生质浓缩、菌丝断裂、抑制病菌分生孢子的萌发、导致孢子芽管扭曲。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Chinese Cinnamaldehyde Cinnamaldehyde alias, Lauraceae plants are dry skin and cinnamon bark obtained by steam distillation of volatile oil in the main component of research in recent years are more root canal sterilization drug, it can seriously undermine the bacterial cell surface structure, and thus has a wider antibacterial spectrum and strong antibacterial activity; gallnut hundred, also known as worms, insect species are Schlechtendalia Schlechtendalia or times aphid eggs in their parasitic plants Anacardiaceae Rhus chinensis, Green bran Yang Yang, such as bran or red tree gall formation, the main ingredient for gallnut tannin, can solidification microbial protoplasts and a variety of body enzymes, a wide range of bacteria, fungi, yeasts are inhibited.

中药桂皮醛别名肉桂醛，是樟科植物肉桂的干皮及树皮经水蒸气蒸馏得到的挥发油中的主要成分，是近年来研究较多的根管消毒药物，它能够严重破坏细菌细胞表面结构，从而具有较广的抗菌谱和较强的抗菌活性；五倍子又名百虫，是倍蚜种昆虫角倍蚜或倍蛋蚜在其寄生漆树科植物盐肤木、青麸杨或红麸杨等树上形成的虫瘿，其主要成分为五倍子鞣质，能够凝固微生物体内的原生质及多种酶，对多种细菌、真菌、酵母菌都有明显的抑制作用。

The complex, semifluid, translucent substance that constitutes the living matter of plant and animal cells and manifests the essential life functions of a cell. Composed of proteins, fats, and other molecules suspended in water, it includes the nucleus and cytoplasm.

原生质一种复杂的，半流动的，半透明的物质，是植物和动物细胞中的活性成分，是细胞生命现象的主要表现由蛋白质，脂肪和其它悬浮在水中的分子构成，包括细胞核和细胞质

Transient assay by bombardment of onion epidermal cells and by PEG mediated gene transter to rice protoplast showed that OsSPX::GFP fusion proteins are located at different subcellular partitions, implying the different functional mechanisms for different family members.

此外，洋葱表皮和水稻原生质体的瞬间表达分析发现，不同的OsSPX与GFP的融合蛋白定位于不同的亚细胞部分，预示着不同的水稻SPX家族成员有不同的作用机制。

54 Is not only much more systematically presented in these older writings, but gives ample evidence of a continuous cabalistic tradition; inasmuch as the mystic literature of the geonic period is only a fragmentary reproduction of the ancient apocalyptic writings, and the saints and sages of the tannaic period take in the former the place occupied by the Biblical protoplasts, patriarchs, and scribes in the latter.

一项研究的少数几个仍然存在猜测书籍披露的事实，忽略了最现代作家对cabala和essenism认为，&神秘传说&，偶尔提及，在talmudic或米大示解经文学（比较聪茨，&货车&二维版，第172页起；若埃尔阿，&万religionsphilosophie苏哈尔&页45-54岁），不仅更系统地介绍了这些年纪较大的著作，而是充分证明了一个连续cabalistic传统，因为神秘文学的若尼克期只是一个残缺不全的复制古代世界末日的著作，和圣人和智者的tannaic期间采取在原地点占领圣经原生质体，长者，文士在后者。

The writer gave a detailed description of Graminaceae tissue culture history, analysized the applied tendencies and prospects about Graminaceae tissue culture basic research and, on author's work basis, pointed out the major factor influencing cereals protoplast cul...

本文拟在评述近年来禾本利植物组织培养(主要指胚胎培养、器官培养、细胞培养和原生质体培养)的理论性研究和应用性研究的进展，并重点描述和讨沦在应用上较为成熟和有发展前景的几个领域的发展现状以及利用禾本科植物的组织培养技术而进行的基因转移技术的概况。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术，是根据植物细胞具有全能性的理论，利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织（如形成层、表皮、皮层、髓部细胞、胚乳等）或细胞（如大孢子、小孢子、体细胞等）以及原生质体，在无菌和适宜的人工培养基及光照、温度等人工条件下，能诱导出愈伤组织、不定芽、不定根，最后形成完整的植株的学科。

He terminal cell apoptosis depended on the time of complete degradation of protoplast in cell.During PCD process,all kinds of cell organelles in protoplast promoted secondary wall deposition at different degree.

EM观察结果表明纤维、基本薄壁组织细胞、导管分子的分化过程均是典型的衰退过程，它是由次生壁的沉积和原生质体的衰退这两个密切相关的过程组成。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！