原生质的

The effect of crude chitinase on three pathogens showed that it could degrade the cell-wall of thepathogens.The crude chitinase could make Fusarium spp and Alternaria spps cell-wall thin andrupture,cell-wall and bioplasm separate,the bioplasm of Rhizoctonia solani distribute asymmetry andbecome vacuole,the cell-wall thin and rupture also.

几丁质粗酶对三种病原真菌菌丝细胞壁的降解作用显示，几丁质酶可使交链孢和镰刀菌菌丝细胞壁变薄、溶解、质壁分离、原生质膨大；丝核菌原生质体分布不均匀、空泡化、细胞壁变薄并出现断裂现象。

Since Arabidopsis thaliana guard cell protoplast is quite small (6~9μm), it had brought lots of difficulty to protoplast isolating and patch clamping.

由于拟南芥保卫细胞原生质体个体较小(6～9μm)，给分离提取保卫细胞原生质体带来了一定的困难。

Influences of basic media,protoplast density,and ABA concentration on somatic embryogenesis of loblolly pine were studied.

研究了基本培养基、原生质体密度和ABA浓度对火炬松(Pinus taeda L。)悬浮细胞原生质体体细胞胚胎发生的影响。

The factors effecting isolation and culture of apple protoplast from leaves in vitro were studied.

以苹果试管苗叶片为原生质体分离材料，对影响原生质体分离和培养的因素进行了研究。

The circumtances of the protolasts culture of leguminosae and the tisse and protoplasts culture of sainfoin has been reviewed in this paper.

本文还综述了豆科原生质体和红豆草原生质体的培养概况以及影响豆科原生质体培养的几个重要因素。

To find out the suitable medium, the optimization groups of hormone of NAA content and ZT content and the appropriate cultural density, the protoplast of two species of Jujuba trees have been cultivated by taking as subject the protoplast of non-kernel small date and egg-like dates embryonic suspension cells obtained by zymolysis, and by employing different mediums with different combination content of NAA, ZT hormones and culture density of protoplast.

以无核小枣和鸡蛋枣两个枣树品种的胚性悬浮细胞经酶解获得的原生质体为材料，采用不同种类的培养基、不同含量的NAA和ZT激素组合及不同原生质体培养密度对两个枣树品种的原生质体进行培养，以获得适宜的培养基种类、较佳NAA和ZT的激素组合及适宜的培养密度。

The results showed that:The best zymolysis conditions for BS and TV protoplasts release and regeneration through orthogonal experiment were as follows: 1mg·mL~(-1) lysozyme treated BS protoplasts for 1.5 hours at 37℃ and 4 mg·mL~(-1) lywallzyme treated TV protoplasts for 2 hours at 30℃. In these conditions, the number of protoplasts release was respectively 8.87×10~8 units/mL and 8.62×10~7units/mL and the regeneration rate was respectively 54.1% and 29.3%.

研究结果表明：通过正交试验确定BS菌和TV菌原生质体制备过程中的最佳酶解条件分别为：BS菌在37℃酶解温度下，1mg/mL溶菌酶酶解1.5h，原生质体制备个数达到8.87×10~8个/mL，再生率为54.1%；TV菌在30℃酶解温度下，4mg/mL破壁酶酶解2h，原生质体制备个数为8.62×10~7个/mL，再生率为29.3%。

The protoplast fusion between wild plant species L.fendleri of the genus Lesquerella and B.napus was studied in order to transfer the industrial oil gene in L.fendleri into B.napus.

为了把野生优质工业用油植物基因导入到普通栽培油菜品种中，本文报道了野生优质工业用油植物L 。 fendleri的原生质体培养及其原生质体与普通栽培油菜品种原生质体融合。

One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask compound screening, genetic marker test and single clone isolation and continuous passage cultivation.

3确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370〓。

2The protoplast formation and regeneration and fusion conditions of strain ISW330 and AS 1.495 were studied in this thesis. One L-isoleucine high-producing strain SW0370 was screened through protoplast fusion, shake flask screening, genetic marker test and single clone isolation and continuous cultivation for generations.

2确定了亲株ISW330和AS1.495原生质体形成和再生及进行原生质体融合的最佳条件，采用原生质体融合技术最终筛选出一株带有目的遗传标记的L-异亮氨酸高产菌SW0370(Leu~-+Met~-+AEC~r+á-AB~r)。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。