原生质的

Now most of planting varieties and corms used in production come from foreign and lack of varieties breeding of the gladiolus in our country. The study on separation, purification and culture technique of the gladiolus will lay the foundation for the breeding of the gladiolus.

目前我国唐菖蒲的栽培品种和生产用球绝大多数从国外引进，唐菖蒲的品种繁育工作不足，唐菖蒲原生质体分离、纯化及培养技术的研究将为唐菖蒲育种工作奠定基础。

Conversely, the immunofluorescence of FUS, a DNA and RNA binding protein known to be localised exclusively in the nucleoplasm,[18] didnot co-localised with [Pt(L3)Cl]+ staining (Figure 3G and H).

相反，FUS（一种已知排他定位于核原生质中的DNA和RNA结合的蛋白质[18]）的免疫荧光与[Pt(L3)Cl]+染色不共定位(图3G和H)。

For one thing,his body is made of the elemental life substance called protoplasm,found in all living things,though differing somewhat in composition in different species.

例如，他的躯体也是由其他不同生物种类都具有的原生质——一种基本的生命物质所构成。

It consists of deposits of suberin and cutin and ensures that all the water and solutes entering the stele pass through the cytoplasm of the endodermal cells, thus allowing for selective filtering of solutes and control of flow rate.

是由木质素或角质的沉积物所组成。从而使水及溶质只能通过内皮层细胞的原生质体进入维管柱，保证了物质有选择性的通过。

The main results were as follows:①The genomic DNA extraction method from TCK/TCT teliospore was established, which include cell wall lysis of teliospore by alkali solution, destroying teliospore cell wall for releasing nucleic acid from cytoplasma membrain and elimination of the PCR inhibitors such as pigments or polyphenols component.

论文主要研究结果如下：①优化了碱裂解法破冬孢子外壁-CTAB破坏原生质体，释放核酸-微量DNA过滤柱去除色素、多酚等PCR抑制物质的TCK/TCT冬孢子提取方法，排除了冬孢子细胞壁难破DNA难以提取，三甲胺、色素等PCR抑制物质导致假阴性的难题，采用此方法提取的腥黑穗菌DNA完全适用于PCR扩增。

The feasibility of the protoplast mutagenesis for increasing productivity was studied on a Claviceps paspali strain, which was not easy to produce conidia under laboratory conditions.

为了提高在一般条件下不易产生分生孢子的雀稗麦角菌的产碱率，对原生质体诱变育种的条件和方法进行了探讨。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

Here, the accepted animal cell protocol was modified to adapt it to plant cells and the sensitivity to UV-B induced DNA damage was investigated in young and mature leaves of Murraya panicuata.

本研究通过对动物细胞彗星检测方法的改进，利用植物细胞原生质体作为材料，研究了不同发育期九里香叶片对UV-B诱导的DNA损伤的敏感性差异。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！