原生质的

Northern blot analysis showed that the replication level of TMV and T/OMP in protoplast also had no difference.

Northern印迹分析表明TMV和T/OMP在原生质体中的复制水平也没有差异。

This paper at first separated the protoplast from rhizopus and yeast respectively, and then merged the protoplasts of them.

利用分离的根霉、假丝酵母菌种，首先各自进行菌体活化，原生质体制备，然后将两菌进行杂交融合。

The results show that DCR medium is in favor of the formation of somatic embryos from protoplasts.

结果表明，DCR基本培养基最有利于原生质体的体细胞胚胎发生。

The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.

显微观察表明，来自原生质体的胚性胚柄细胞团(ESM：embryogenic suspensor mass)，经早期原胚(ESP：early stage proembryos)阶段形成了后期原胚(LSP：late stage proembryos)。

Results: PC12 cells fragmentation exhibited various evolving phases including totipotential protoplast, endopolyploid,polykaryon, multi-nucleo-endoplast, multinucleocyte, syncytium and multicells. The PCNA-IR was strongly positive in the earlyphase samples of cell fragmentation.

结果：PC12细胞呈现包括全能原生质体、核内多倍体、多核体、核-内质多聚体、合胞体和多细胞裂殖球的一系列演化阶段。

Objective］ The study aimed to explore the protoplast isolation and culture of callus in Apocynum venetum and the influence factors.

［目的］探究罗布麻愈伤组织原生质体的分离培养及其影响因素。

The fourth one is the etheric body, also called the bioplasmic body by some and this one is the fourth of your four lower bodies.

第四个是以太身体，也被某些人称为原生质体而这是四个较低级的身体第四个。

Embryogenic suspension cell is ideal material of protoplast culture of gramineous plants.

胚性悬浮细胞是禾谷类作物原生质体培养的理想材料。

After the fusion-treated protoplasts were cultured in BH3/EME medium for 2 days, pollen tube growing protoplasts were observed.

融合后的原生质体培养于BH3/EME培养基中。2天后，观察到花粉管生长现象。

In this paper, an escherichia coli-saccharopolyspora erythraea shuttle vector containing the erya promoter region was constructed using the enhanced green fluorescent protein gene as a reporter. the shuttle plasmid was transformed into sac.erythraea a226 and streptomyces lividans jt46 by peg mediation, respectively. fluorescence microscopy confirmed that the egfp was expressed in both strains.

本文克隆了erya基因的启动子perya，以绿色荧光蛋白基因为报告基因，构建了大肠埃希菌-糖多孢红霉菌穿梭型质粒。peg介导原生质体转化法将穿梭型质粒分别转入糖多孢红霉菌a226与变铅青链霉菌jt46，荧光显微镜检测发现，此启动子在两菌株中都具有功能。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！