原生质的

The results showed that the Saccharopolyspora spinosa SP06081 protoplast yield was the highest under these conditions: the collected mycelium from SP06081 grown in Tryptic Soy Broth medium with 0.2% glycin for 48 h was treated by 0.1 mg/mL lysozyme at 28oC for 20 min, then plated on the R2YE medium with sucrose as osmotic stabilizer, the number of regeneration protoplast was up to 108/mL. The protoplast-regenerated strains exhibited changes in morphology and antibiotic production, 29.3% protoplast-regenerated strains was characterized by loose mycelium and abundant broken branches as did their parent. Among them, 58.2% strains presented the trend to positive variation in spinosad yield, with the highest spinosad yield of up to 582.0 mg/L, 85.6% higher than that of their parent.

结果表明：菌体在添加0.2%的甘氨酸的TSB培养基中培养48 h收集， 0.1 mg/mL 溶菌酶， 28oC作用20 min制备原生质体，将原生质体涂布于以蔗糖为渗透压稳定剂的R2YE培养基中，原生质体再生数目最多，达108个/mL以上；原生质体再生菌株在形态和抗生素产量上产生分化， 29.3%的再生菌株形态上保持与亲本菌株一致，具有菌丝松散，断裂分枝多的特点，其中53.2%的再生菌株多杀菌素产量变异向正方向移动，最高产量达到582.0 mg/L，比亲本菌株提高85.6%。

Scientists are now applying these laws to problems as diverse as the flow of protoplasm in a cell and the flow of traffic in city

现在科学家们正把这些规律用来解决像细胞中原生质的流动和城市交通流量等这样一些不相同的问题。

Two whiplike threads of protoplasm reduced to a microscopic scale .

原生质的两条的鞭形螺纹；减小到小的比例。

Two minute whiplike threads of protoplasm; reduced to a microscopic scale.

原生质的两条极细的鞭形螺纹；减小到极小的比例。

An unperforated, complete septum forms at the site of nuclear division and bisects the apicall cell, followed by the cytoplasmic streaming and a new branch is initiated.

细胞具有完全、没有孔的隔板，长达数百微米，且具有一个明显的大核，随著细胞生长，原生质的川流趋向细胞顶端转移。

Symbol P A highly reactive, poisonous, nonmetallic element occurring naturally in phosphates, especially apatite, and existing in three allotropic forms, white, red, and black. An essential constituent of protoplasm, it is used in safety matches, pyrotechnics, incendiary shells, and fertilizers and to protect metal surfaces from corrosion.

符号 P 磷：一种易起化学反应的、有毒的非金属元素，常在磷酸盐中天然存在，尤其是磷灰石，以三种同素异形的形式存在，为白色，红色和黑色，是原生质的基本成分，可用于安全火柴、烟花、燃烧弹和化肥，还可以保护金属表面免于腐蚀。

Two whiplike threads of protoplasm reduced a microscopic scale .

原生质的两条极的鞭形螺纹；减小极小的比例。

Minute whiplike threads of protoplasm reduced to a microscopic .

原生质的条极细的鞭形螺纹；减小到极小的。

In them were no considerations of Mediterranean cruises, of soporific Southern skies or drifting in the Vesuvian Bay.

他拐到百老汇路，在一家灯火辉煌的餐馆门前停了下来，每天晚上，这里汇集着葡萄、蚕丝与原生质的最佳制品。

Second, procedures of effective protoplasts fusion and U.V. irradiation or heat treatment protoplasts fusion were developed. Equal protoplast suspensions (containing about 108/ml protoplasts) of each strain were mixed and washed by centrifugation, then the pellets were resuspended in PEG4000 solution (35%) at 37癈 for 2min.

其次，确定了原生质体融合的基本条件及灭活原生质体融合的条件：等量混合两亲株原生质体或用热灭活的原生质体(＞10~8个／ml)，用35％的PEG4000在37℃处理2min后，稀释，涂布再生，可获得融合率在50％左右的融合子。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。