原生质球

RESULTS Hp can grow in liquid media in room temperature, and 3 out of 18 strains of coccoid Hp reverted to bacterial.

结果 Hp可在室温液体中繁殖，18株中观察到3株球形体Hp在室温下的返祖，除常见球形变外的Hp杆状弯曲、长杆状、短杆状、长丝体、巨球体、丝球体、原生质体及细小"S"和弧形变异。

There were also a number of protoplasm body appeared in the perivitelline, especially in the area near the first polar body.

在卵周隙尤其是在第一极体的旁边，有许多原生质球存在。

Results: PC12 cells fragmentation exhibited various evolving phases including totipotential protoplast, endopolyploid,polykaryon, multi-nucleo-endoplast, multinucleocyte, syncytium and multicells. The PCNA-IR was strongly positive in the earlyphase samples of cell fragmentation.

结果：PC12细胞呈现包括全能原生质体、核内多倍体、多核体、核-内质多聚体、合胞体和多细胞裂殖球的一系列演化阶段。

The spheroplast suspension (200 ul) was transferred into a microcentrifuge tube, supplemented with 150–350 units/ml micrococcal nuclease (Fer- mentas), and incubated at 37°e for 5 min.

暂停的原生质（ 200 UL ）的是转入微量管，补充与150-350单位/毫升微球菌核酸酶，并培养在37 ° E的5分钟。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

Oenococcus oeni,an important microorganism during wine making could convert malic acid into lactic acid,and develop wine flavor and enhance the stability of biology.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。