原生质体

RAPD analysis indicated that the profiles of mycelial protoplasts obtained from same single spore isolates amplified by 10 random primers were entirely identical, corroborating that the two nuclei of a basidiospore were homogeneous.

RAPD分析显示，源于同一单孢分离物的菌丝原生质体为10个随机引物所扩增的图谱彼此完全相同，印证了担孢子中的双核是同质的。

Besides from nature depart is chosen besides, lure change what breeding reforms technology of technology, bioplasm body confluence and gene operate a technology to apply Yu Mu mould effectively is improved in, had had the commercial preparation of a variety of 10 wood mould at present.

除了从自然界分离筛选之外，诱变育种改良技术、原生质体融合技术以及基因操作技术已有效地应用于木霉菌的改良中，目前已经有10多种木霉菌的商品化制剂。

This BIOPLAST Nose Ring is a timeless and classic nose jewelry item.

这原生质体鼻环是永恒的，经典的鼻子珠宝项目。

This push-in style bioplast replacement nose ring can be used with any 18 gauge push-fit nose ring..

这推入式风格的原生质体更换鼻声，可用于任何18号推适合鼻环。。

Keywords: Calcium signaling;phytohormone;light;protoplast

中文关键词：钙信使系统；植物激素；光；原生质体

These results showed that plant cell wall and cytoplasm membrane is the coat system of plant cell.

植物细胞包被系统的酸性降解使植物细胞器重组并产生新的原生质体成为可能。

The protoplasts were purified and regenerated through single layer plate method in cellobiose medium with 0.6M mannitol used for maintaining osmotic pressure.

此外，优化了灵芝的电激转基因方法，选择最佳的电激参数，将hph基因导入灵芝基因组中，转化效率达到约50个转化子／10〓个原生质体。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

The prepared protoplasts could regenerate mycelium and produce conidium with similar pathogenecity to that of parental strains.

所制备的原生质体均能再生菌丝和具有产生与原菌株相同致病性分生孢子的能力。

The response of plant DNA to UV-B radiation has certain sensitivity which related to UV-B radiation intensity and plant cytoplast, showed specific dose-effect relationship.

植物DNA对UV-B辐射的响应具有一定的敏感性，这种敏感性与不同的UV-B辐射剂量和不同植物细胞的原生质体有关，表现出一定的剂量－效应关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。