原生质体

In part of edible fungi, two different nuclei in heterokaryon are generally consided to distribute evenly to all of colony through clamp connection, and the ratio of two nuclei should be 1:1. But other hypothesis was demurrable according aberrancy of nucleus ratio during monokaryotization of oidiospore and protoplast in F. velutipes. My experiment was constituted of two parts.

在部分食用菌中，一般认为异核体菌丝通过锁状联合这一细胞结构将两种核均匀分布到全部菌落，异核体中两种核的数量比例应该是1：1的关系；但金针菇原生质体单核化和粉孢子单核化过程中发生的核偏离的研究表明，异核体菌丝两种核的数量比例可能不是1：1的关系。

The effect of crude chitinase on three pathogens showed that it could degrade the cell-wall of thepathogens.The crude chitinase could make Fusarium spp and Alternaria spps cell-wall thin andrupture,cell-wall and bioplasm separate,the bioplasm of Rhizoctonia solani distribute asymmetry andbecome vacuole,the cell-wall thin and rupture also.

几丁质粗酶对三种病原真菌菌丝细胞壁的降解作用显示，几丁质酶可使交链孢和镰刀菌菌丝细胞壁变薄、溶解、质壁分离、原生质膨大；丝核菌原生质体分布不均匀、空泡化、细胞壁变薄并出现断裂现象。

RESULTS Hp can grow in liquid media in room temperature, and 3 out of 18 strains of coccoid Hp reverted to bacterial.

结果 Hp可在室温液体中繁殖，18株中观察到3株球形体Hp在室温下的返祖，除常见球形变外的Hp杆状弯曲、长杆状、短杆状、长丝体、巨球体、丝球体、原生质体及细小"S"和弧形变异。

Besides coccoid of Hp transformations, curve-bacilliform, long-bacilliform, short bacilliform, long threadlike, giant coccoid, glomerule, trophoplast, and very small "s" or "c" shaped Hp were found.

并根据Hp形态变化的顺序及表型，初步发现Hp由典型"S"或多螺旋形态变为杆状弯曲、长杆状、短杆状、然后变为球形、长丝体、巨球体、丝球体、原生质体的规律。

Hygroscopicus FC904 were treated with lysozyme and the numbers of protoplasts under different conditions were compared.

方法用溶菌酶处理S.hygroscopicusFC904的菌丝体，比较在不同条件下原生质体的形成数。

Methods Mycelia of S.hygroscopicus FC904 were treated with lysozyme and the numbers of protoplasts under different conditions were compared.

用溶菌酶处理S.hygroscopicus FC904的菌丝体，比较在不同条件下原生质体的形成数。

The isolated nori cells or protoplasts can be cultivated into thalli for the totipotency of plant.

由于植物细胞的全能性，解离的单细胞或原生质体经培养能够正常发育成叶状体。

And on the basis of this protocol,protoplast electrofusionof cabbage with radish was studied to find out the condi-tions and parameters affecting electrofusion.

本研究通过对甘蓝子叶、萝卜子叶和下胚轴原生质培养的研究，建立了甘蓝与萝卜原生质体培养再生植株体系。

The results show that the suitable conditions are enzyme concentration 5mg/mL, my-celium age 24h, zymolytic temperature 37 ℃, time 1h. The protoplast formation rate is 100%, The regeneration rate is 64.6%.

结果表明：出芽短梗霉原生质体制备所需的最佳条件是酶浓度5 mg/mL、菌龄24 h、酶解温度37℃、酶解时间1 h，在此条件下原生质体形成率达100%，再生率为64.6%。

Then, they were transferred into the mycelium culture fluid which includes 30% sucrose and 1% or 1.5% glycin under the condition of 20℃, 150 rpm for 48 h. In order to obtain protoplasts of mass and high quality, the strain F46 protoplasts given after combination use of the 10 mg·mL-1 lysozyme and 10 mg·mL-1 snailase at 34℃for 3 h; and the SC1 was treated by 10 mg·mL-1 lysozyme at 34℃for 2.5 h.

确定了双亲原生质体形成与再生的最佳条件：菌株F46、SC1采用二次菌丝培养，即在蔗糖含量20%菌丝培养液里，25℃条件下150 rpm培养48 h；然后按15%的接种量转接到蔗糖含量30%，甘氨酸含量分别为1%、1.5%的菌丝培养液中，同样条件下，继续培养48 h；在34℃条件下，菌株F46用等量混合的蜗牛酶和溶菌酶20 mg·mL-1，菌株SC1用10 mg·mL-1溶菌酶，分别酶解3 h、2.5 h，不仅有大量的原生质体形成，且再生率也高。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。