原生质体

Results: PC12 cells fragmentation exhibited various evolving phases including totipotential protoplast, endopolyploid,polykaryon, multi-nucleo-endoplast, multinucleocyte, syncytium and multicells. The PCNA-IR was strongly positive in the earlyphase samples of cell fragmentation.

结果：PC12细胞呈现包括全能原生质体、核内多倍体、多核体、核-内质多聚体、合胞体和多细胞裂殖球的一系列演化阶段。

Studies on the mutant breeding of high-yield gibberellins strain The protoplast of Gibberella fujikuroi m978 were treated by mutation breeding forhigh-yield gibberellins, LiCl mutation was better than DES mutation or UV irradiation ifsingle mutation, but any single mutation was worse than compound mutation by whichthe mutants were more overproducting and genetically stable.

采用传统诱变育种，筛选高产菌株在原生质体条件下，对赤霉素产生菌m978的原生质体进行菌种选育；使用单因子诱变方法，LiCl处理的效果优于DES诱变和UV诱变；复合诱变比单因子诱变更容易得到高产突变株，且获得的高产突变株稳定性更优。

In order to use the techniques of tissue and protoplasts culture for the plant breeding, it i prerequisite to understand the morphogenic potential of different varifies in tissue culture, and set up an efficient protoplast system capable of sustained divisi〓n and plan〓 r〓generation.

为了将组织培养和原生质体培养技术用于育种目的，必须了解不同品种的红豆草在组织培养中的再生能力和建立一个高效率的原生质体培养与再生的实验系统。

Adding fresh medium to cultures, when they had been cultured for 5-8 days, could promote cell sustained division, after which, adding a medium with lower concentration osmatic substance or without osmatic one could futher stimulate the cell division and ensure the active growth of the cell masses.

采用液体浅层培养方法，对埃斯基和雷蒙特红豆草的原生质体进行了培养。埃斯基花茎愈伤组织的原生质体在VK和Kao＇s培养基中2-6天分裂，2周内形成细胞团，19天时的分裂率和克隆率分别为71.41-76.19％、64.78-67.03％。

Using of agarose-embedment lysis cell wallmethod for preparing intact chromosomal DNA from phytopathogenic fungi is thefirst time.

首次采用凝胶包理破壁法由植物病原真菌分生孢子获得完整染色体DNA，克服了先用裂解酶破壁获得大量原生质体再用琼脂糖包理原生质体的传统制备方法的一些不足。

Cell wall formation and division of protoplasts derived form E. radiata suspension cells were observed. However, division did not progress beyond this stage and the protoplasts died after one month.

来自放射桉悬浮细胞之原生质体培养，可观测到原生质体分裂及细胞壁的形成，唯经培养1个月后，并未再进一步的发展而渐死亡。

On the basis of protoplast culture, we performed protoplast fusion between Astragalus melilotoides Pall, and Zygophyllum xanthoxylum Maxim using a modified PEG high-pH, high-Ca2 method. The protoplasts of A. melilotoides were pretreated with Rhodamine-6G and those of Z.

在成功培养原生质体的基础上，用改进的PEG-高pH高钙法诱导草木樨状黄芪和木本霸王原生质体融合，得到了科间体细胞杂种融合细胞。

In the mating test, mycelial protoplast pairs between single spore isolates of different strains formed colonies with clamp connection, while mycelial protoplast pairs between the same single spore isolates formed colonies without clamp connection, suggesting that the two nuclei of the spores possessed identical mating type factors.

在交配试验中，源于不同菌株单孢分离物的菌丝原生质体的配对形成具锁状联合的菌落，而源于同一单孢分离物的菌丝原生质体的配对则形成无锁状联合的菌落，暗示担孢子中的两个核具有相同的交配型。

The results showed that about 3.18 x l0~7/ml of protoplasts were formed when the mycelia were incubated for 1 hour at 28 °C in TSB medium containing 2% glycin, and treated with 2 mg/ml lysozyme.

对东方拟无枝酸菌原生质体制备、再生及质粒DNA转化等条件进行了探索，考察了培养基组分、甘氨酸浓度、溶菌酶浓度等因素对原生质体的制备及其再生的影响。

To improve spinosyn-producing strain and enhance spinosyns yield, we studied the effects of glycin concentration and the operational time, temperature and lysozyme concentration on protoplast preparation of Saccharopolyspora spinosa SP06081. We also studied different regeneration media and osmotic stabilizing agents.

为了改良多杀菌素生产菌种，提高多杀菌素产量，研究了甘氨酸添加浓度、溶菌酶作用时间、温度和浓度对多杀菌素生产菌刺糖多胞菌Saccharopolyspora spinosa SP06081菌株原生质体制备和再生的影响，并考察了不同再生培养基和渗透压稳定剂对其再生的影响，确定了该菌株原生质体制备和再生的最佳条件。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。