原生质体

Since Arabidopsis thaliana guard cell protoplast is quite small (6~9μm), it had brought lots of difficulty to protoplast isolating and patch clamping.

由于拟南芥保卫细胞原生质体个体较小(6～9μm)，给分离提取保卫细胞原生质体带来了一定的困难。

Influences of basic media,protoplast density,and ABA concentration on somatic embryogenesis of loblolly pine were studied.

研究了基本培养基、原生质体密度和ABA浓度对火炬松(Pinus taeda L。)悬浮细胞原生质体体细胞胚胎发生的影响。

According to the results of morphological, cytological and molecular identification, it was proved that cybrids were obtained from 7-0-8-4+66-3 fusion combination, and the petaloid cytoplasmic male st

论文对影响胡萝卜原生质体非对称融合的若干因子、组培茵的先期抽墓、利用非对称融合转移胡萝卜瓣化型雄性不育性的可行性、本研究存在的问题及改进方案以及非对称原生质体融合在胡萝卜资源创新中的应用价值等进行了讨论。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In addition, the application of bacteria protoplast fusion technique in genetic breeding was reviewed in the article and the development of this technique was looked forward to.

介绍了细菌原生质体融合技术在遗传育种中的应用，并展望了细菌原生质体融合技术的发展前景。

The factors effecting isolation and culture of apple protoplast from leaves in vitro were studied.

以苹果试管苗叶片为原生质体分离材料，对影响原生质体分离和培养的因素进行了研究。

The result showed that the rate of production and their frequency of protoplasts division were strongly dependent on the state of the calli which were isolated by enzymatic method.

研究结果表明，酶法分离原生质体的产率和分裂频率明显取决于用于制备原生质体的愈伤组织的状态。

Plant protoplasts from several species of plant were fused successfully by electrofusion method, and a good procedure for electrofusion of plant protoplasts in wideinterval chamber was established according to the results.

在植物原生质体的电融合研究中，着重进行了甘蓝与大白菜原生质体的杂交融合和异核体培养，并建立了实用的电融合方法和操作程序

In order to improve the ability of degrading anthracene and the survival of exogenous bacteria An, protoplast electrofusion was performed between An which can degrade anthracene effectively and Tu which has high survival ability, and then a fusant with specific properties was selected from the regenerated colonies.

为了提高外源蒽降解菌An降解蒽的能力和存活能力，采用原生质体电融合技术，在蒽降解菌An和土生优势菌Tu之间实行了原生质体电融合，并从再生的融合子F中筛选到具有特定性状的后代菌株。

Honghezi,Dongbi,Shieryuelongyan,etc.The results showed that the method of Calcium-alginate -embedded suspended culture shaking at50r/min was suitable for longan proto plast culture;and the optimal regeneration,with up to5%of microcolony formation,occurred when protoplasts from suspensions were cultured ...

研究结果表明，海藻酸钙包埋悬浮振荡培养(50r/min)是龙眼悬浮细胞原生质体培养的适宜培养方式，在含有多种生长调节剂的改良MS培养基(MP6)上，再生小克隆的形成频率可高达5%；采用龙眼胚性愈伤组织体胚诱导、成熟和萌发的优化方案，原生质体再生小克隆分化子叶形胚状体的频率达100%，萌发植株的频率一般大于45%。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。