原生质体

The impacts of urediniospore activity, concentration of hydrochloric acid and physiological races of rust fungi on protoplast shrinking were also studied.

研究还发现叶锈菌和秆锈菌夏孢子活力、盐酸浓度等对夏孢子原生质体浓缩状况有很大影响，且与病菌生理小种有一定的关系。

The focus of this dissertation work is to investigate possible involvement and signal transduction mechanisms of cAMP in regulation of Vicia stomatal movements.

论文的第一部分，从蚕豆叶片表皮组织水平和气孔保卫细胞原生质体K〓通道水平确定cAMP参与了ABA或Ca〓对气孔运动的调节。

Viciaefolia Scop had been cultured and produced calli, and 〓lantlet were regenereded from protocalli of O.

对3个品种(O.viciaefolia)的原生质体进行了培养均得到了愈伤组织，并得到了埃斯基var。

Then, in term of single parent or bi-parent inactivate, the protoplast fusion were carried out by PEG 6000 at concentration of 40%, in water bath of 34℃for 10 min.

按不同的组合方式，将双亲的原生质体等量混合，经40%PEG助融10 min，培养再生，获得40株融合子。

Using Zhongshuang4 as a recurrent parent to pollinate the male sterile plants, the ratio of male sterile plants increased with the number of backcrosses.

利用原生质体融合技，获得了甘蓝型油菜品种中双4号与新疆野生油菜野油18的对称性体细胞杂种。

Chromosome of identified haploids were reduplicated, identified, and thereafter pure diploids were obtained.

本项目还对河套蜜瓜的细胞和原生质体培养进行了研究，并获得了再生植株。

In addition, we compared the change of morphology and spinosyns yield of the regenerated strains.

同时， 对原生质体再生菌株的形态与多杀菌素产量变化进行了比较研究。

The new yeast strain HU-KDF-185 screened from interspecific fusants between Saccharomyces cerevisiae and S.diaslaticus demonstrates a high-yield producer of ethanol, The strain produces 30.77% of ethanol on account of fermented materials when it ferments saccharified liquid (about 5.0-5.5 Brix) prepared from dried sweet potato-residuces after making starch in the productive fermentation test of Xian Ju distillery.

从原生质体融合获得的K氏酵母(Saccharomyces cerevisiae var.ellipsoideus)和糖化酵母的种间融合杂种中筛选到一株酒精高产菌株HU-KDF-185，它在番薯渣糖化液(约5.0—5.5Brix)的工厂生产性发酵试验中，原料的酒精得率平均是30.77％；而当前使用的酒精生产菌株K氏酵母(对照1)则是29.71％，新市一号菌株(对照2)则是29.54％。

Based on the results of information investigation, the paper briefly summarizes the research and application of cell engineering technology in agriculture: Chromosome engineering,protoplast culture,anther culture, cell culture, tissue culture and somatic hybridization. The worldwide development and progress in the application of cell engineering in plant breeding are reviewed.

根据文献研究结果，系统地综述了染色体工程技术、原生质体培养、花药培养、细胞培养与无性系筛选、组织培养与体系胞杂交等细胞工程技术，在国内外农作物育种上开发应用后所取得的新进展、新成果，以及这些新成果的产业化，对促进中国农业生产的发展所起的作用。

The course mainly introduces basic principles and methods of experimental techniques of cell engineering, which includesmedia preparation, sterilization and aseptic technique, virus-free seedling production, plant rapid propagation, immature embryo culture, pollen and anther culture, cell culture, protoplast culture, somatic hybridization, plant transformation, and so on. The contents of this course follow the from easy

该课程主要介绍细胞工程特别是植物细胞工程的基本实验技术及其原理与方法，包括：培养基配制、灭菌及无菌操作技术、无病毒苗培育、植物快速繁殖、幼胚培养、花药及花粉培养、细胞培养、原生质体培养、体细胞杂交及植物遗传转化等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。