原生质体

When the protoplast-derived calli were transferred into the MS solid medium added with 2.0mg/L benzylamino-purine and 0.4mg/L naphthaleneacetic acid and cultivated in the codition of low illumination, after which the adventitious shoots were induced, then we could obtain numerous plantlets and could transplant them survival to pots in the greenhouse.

当将原生质体分裂形成的愈伤组织转移到附加2.0mg/L6-苄氨基嘌呤和0.4mg/L萘乙酸的MS固体培养基上，并在低光照条件下培养后，从愈伤组织上分化出了不定芽，进而发展成小植株，并移栽成活。

In this study, the whole tissuecultivating system of Mozzie buster and Ocimum basilicum was established, and thecondition of protoplasts fusion was primarily analyzed. The results of our experimentwere shown as follows: 1. MS was used as the basic cultivating medium.

本实验建立了完善的驱蚊草、罗勒组培体系，并对驱蚊草、罗勒原生质体融合的条件进行了初步分析，主要结果如下： 1。

High palmitoleic acid-producing strains were obtained by protoplast fusion between Saccharomyces cerevisiae No.12.926 producing palmitoleic acid and Rhodotorula No.12.908 producing oil and fat.

采用原生质体融合技术进行产棕榈油酸酵母Saccharomy cescerevisiaeNo.12.926和产脂酵母RhodotorulaNo.12.908的融合研究，获得了棕榈油酸高产酵母工程菌株。

In this study, protoplast was used as receptor for transgenic research in order to construct an effective transgenic system for Porphyra yezoensis.

为此，我们以条斑紫菜的原生质体为受体，进行转基因研究，初步建立其转基因体系。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.

通过对预处理剂质量浓度、酶质量浓度、酶解时间、菌龄等影响因素的分析，以及渗透压稳定剂的筛选，对酒酒球菌SD-2a原生质体的制备条件进行了研究。

This paper studied the conditions of protoplast preparation of Oenococcus oeni,including the concentration of preculture and lysozyme,lytic time,hypha culture time,etc.As a result,hypha culture time 20 h,penicillinG Na 0.5 μg/mL,lysozyme 1 mg/mL,lytic time 30 min,SMM as osmotic pressure stabilizer,ATB culture medium of regeneration culture mediu...

结果表明，酒酒球菌SD-2a原生质体制备的最佳条件为：菌龄20 h，青霉素G钠质量浓度0.5μg/mL，酶质量浓度1mg/mL，酶解时间30 min，渗透压稳定剂采用SMM缓冲液，再生培养基采用添加0.7 mol/LKCl的ATB培养基，再生培养时采用双层培养基厌氧培养，可以保证较好的形成率和再生率。

The effect of the toxin on protoplasmic membrane protein was studied by the method of fluorescence, using 8-anilino-1 -naphthalene-sulfonic acid as probe and taking the mesophyll protoplasts from maize leaves as the research system.

以玉米叶片原生质体为研究对象，以ANS(1-苯胺基-8-萘磺酸)作荧光探针，用荧光法研究了Ⅱ-3与质膜蛋白的互作。

Abstract］ Objective: To provide a suitable fungal material for protoplast-mediated genetic transformation and mutation induction of Pythium guiyangense Su.

目的：建立贵阳腐霉原生质体的制备和再生系统，为该菌的基因工程和细胞工程改良提供适宜的真菌状态。

The highest yield of the two eucalypts from cell suspension cultures differed: 4.7×10^6/mL for E. macarthurii and 6.9×10^6/mL for E. radiata.

二树种由悬浮细胞分离原生质体的产量差异颇大，毛皮桉为4.7×10^5/mL，放射桉为6.9×10^6/mL。

Digitatum were released from the hyphalapex, occasionally releasing from the subapical or original sites.

原生质体释放有顶端、侧端和原位释放3种方式。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。