原生质体

The writer gave a detailed description of Graminaceae tissue culture history, analysized the applied tendencies and prospects about Graminaceae tissue culture basic research and, on author's work basis, pointed out the major factor influencing cereals protoplast cul...

本文拟在评述近年来禾本利植物组织培养(主要指胚胎培养、器官培养、细胞培养和原生质体培养)的理论性研究和应用性研究的进展，并重点描述和讨沦在应用上较为成熟和有发展前景的几个领域的发展现状以及利用禾本科植物的组织培养技术而进行的基因转移技术的概况。

The protoplast fusion between hyperparasitic Streptomyces strain F46 and Actinomyces strain SC11 was studied in order to improve the effect of wild actinomyces strains to control plant diseases, solve the problems in application of the biocontrol strains, explore the method of protoplast fusion and get the recombinants with some excellent characteristics of both parents.

为了提高野生生防放线菌株的防病效果，克服以往生防菌应用方面存在的诸多弊端，探索简单易行的人工改良生防放线菌的途径，我们选用重寄生链霉菌F46和放线菌SC11作为亲本，进行了双亲原生质体融合研究，以期得到整合双亲多种优良性状的工程菌株。

The protoplast fusion between hyperparasitic Streptomyces F46 and actinomycetes SC1 and SC11 was studied for getting the fusants recombined excellent characteristics of both parents.

为了提高现有生防放线菌的综合性状，试验将本研究室分离保存的3种各具不同优良性状的生防放线菌F46、SC1和SC11作为亲本，开展双亲和3亲本的原生质体融合，通过研究，获得了综合亲本优良性状的新型高效生防菌株。

The excellent characters of the hyperparasitic Streptomyces F46 and the Streptomyces SC1 were tried to integrate into a fusant by protoplast fusion. The study mostly included content as follows: First, the parental strains were identified by two approaches of the classic identify and the molecular taxonomy.

本试验利用原生质体融合技术将重寄生链霉菌F46和链霉菌SC1融合，以期得到整合双亲优良特性于一体的生防菌。

Protoplasts were isolated from cotyledons of eggplant (S. Melongena cv. Yuanjing) in a solution containing 0.75% cellulase R-10, 0.2% Hemicellulase Rhozyme and 0.2% Pectinase.

将茄子子叶原生质体放在0.75％纤维素酶R-10、0.2％半纤维素酶Rhozyme和0.2％果胶酶溶液中分离。

The efficient and economical tool enzymes for isolating protoplasts of Porphyra yezoensis from the digestive glands of four shellfish (Monodonta labia, Chlorostoma rusticum, Littorina brevicula, Acanthochiton rubrolineatus), cellulase and pectinase from internal market were studied.

通过比较自制的4种海螺酶（分别取自单齿螺、锈凹螺、短滨螺和石鳖）及国产的纤维素酶、果胶酶等对条斑紫菜细胞壁的降解效果，探索经济高效制备条斑紫菜原生质体的工具酶，并进一步研究了最佳酶解条件。

This condition may be ralated to the source tissue which had lost morphogenic potential.

原生质体的愈伤组织在继代中生长旺盛，繁殖迅速，但无形态发生，这可能与起始材料本身已丧失分化能力有关。

According to morphous characteristics,it was regarded as Phanerochaete chrysosporium.

由正交试验确定出该菌原生质体制备和再生的最佳条件：酶浓度为1.5mg/mL，酶解时间为2h，酶解温度为30℃。

Furthermore,the changes of cell organelles realized different cell functions, which fiber for mechanical support,parenchyma cell for preservation and vessel for transportation.3.FE-SEM and DCR technique revealed the arrangements of CMfs on cell wall of fiber, parenchyma cell and vessel.Firstly,fiber cell formed multilamellate concentric structure with thin and thick lamellae alternate.

纤维、基本薄壁组织细胞、导管分子的终端分化就是PCD；并且PCD过程是一个具有较长周期的过程，周期长短比较为基本薄壁组织细胞＞纤维＞导管分子；三类细胞最后的凋亡决定于细胞内原生质体完全降解的时刻。

Here, the accepted animal cell protocol was modified to adapt it to plant cells and the sensitivity to UV-B induced DNA damage was investigated in young and mature leaves of Murraya panicuata.

本研究通过对动物细胞彗星检测方法的改进，利用植物细胞原生质体作为材料，研究了不同发育期九里香叶片对UV-B诱导的DNA损伤的敏感性差异。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。