原生质体

The results show that DCR medium is in favor of the formation of somatic embryos from protoplasts.

结果表明，DCR基本培养基最有利于原生质体的体细胞胚胎发生。

It consists of deposits of suberin and cutin and ensures that all the water and solutes entering the stele pass through the cytoplasm of the endodermal cells, thus allowing for selective filtering of solutes and control of flow rate.

是由木质素或角质的沉积物所组成。从而使水及溶质只能通过内皮层细胞的原生质体进入维管柱，保证了物质有选择性的通过。

The microscopical observation showed that embryonal suspensor masses developed firstly into early stage proembryos,then into late stage proembryos.

显微观察表明，来自原生质体的胚性胚柄细胞团(ESM：embryogenic suspensor mass)，经早期原胚(ESP：early stage proembryos)阶段形成了后期原胚(LSP：late stage proembryos)。

The main results were as follows:①The genomic DNA extraction method from TCK/TCT teliospore was established, which include cell wall lysis of teliospore by alkali solution, destroying teliospore cell wall for releasing nucleic acid from cytoplasma membrain and elimination of the PCR inhibitors such as pigments or polyphenols component.

论文主要研究结果如下：①优化了碱裂解法破冬孢子外壁-CTAB破坏原生质体，释放核酸-微量DNA过滤柱去除色素、多酚等PCR抑制物质的TCK/TCT冬孢子提取方法，排除了冬孢子细胞壁难破DNA难以提取，三甲胺、色素等PCR抑制物质导致假阴性的难题，采用此方法提取的腥黑穗菌DNA完全适用于PCR扩增。

In this dissertation, firstly, the conditions of isolation and purification of specific toxin fractions produced by Exserohilum turcicum has been studied, and a high-efficient method has been established;secondly, the toxins were analysed by UV-Vis spectrophotometry;then the effect of specific toxin on death rate of corn leaves protoplast was studied by FDA as a tinct reagent;finally, using ANS as a probe and SDS-PAGE to study the membrane protein of the corn leaves with Htl gene. In a word, the aim of the research is to search some existent evidences of the toxin binding site on protoplasmic membrane, and provide proofs for nosogenesis of specific toxin in terms of cytology and the molecular biology.

本研究对玉米大斑病菌1号小种毒素的特异性组分进行分离，建立了高效的特异性毒素分离纯化方法，并对其进行了紫外波谱分析；用荧光素双醋酸酯染色法，测定了毒素特异性组分对原生质体死亡率的影响；进而用1-苯胺基-8-萘磺酸荧光探针标记法和SDS-PAGE电泳法对特异性毒素组分与Htl基因玉米细胞互作过程中的质膜蛋白进行了研究，旨在证明Htl基因玉米细胞质膜上存在特异性毒素组分结合蛋白，为从细胞和分子生物学角度阐明毒素的致病机理提供证据。

Transient assay by bombardment of onion epidermal cells and by PEG mediated gene transter to rice protoplast showed that OsSPX::GFP fusion proteins are located at different subcellular partitions, implying the different functional mechanisms for different family members.

此外，洋葱表皮和水稻原生质体的瞬间表达分析发现，不同的OsSPX与GFP的融合蛋白定位于不同的亚细胞部分，预示着不同的水稻SPX家族成员有不同的作用机制。

Objective］ The study aimed to explore the protoplast isolation and culture of callus in Apocynum venetum and the influence factors.

［目的］探究罗布麻愈伤组织原生质体的分离培养及其影响因素。

Using turnip rape as material,mesophyll protoplasts were isolated from 5-8 day-oldleaves of axenic shoots.

以芜菁油菜为材料，从无菌苗5—8天叶龄的叶片分离叶肉原生质体，然后以6×10￣4／mL的密度用MP1－3液体培养基进行浅层培养。

The fourth one is the etheric body, also called the bioplasmic body by some and this one is the fourth of your four lower bodies.

第四个是以太身体，也被某些人称为原生质体而这是四个较低级的身体第四个。

Change in the concentration of endogenous IAA was measured with enzyme-linked immunosorbent assay, and the distribution of IAA-binding protein in the protoplasts in the cambial region was tested with an immuno-fluorescence technique, being improved by the authors, during the cambial active period in Broussonetia papyrifera L.

构树（Broussonetia papyriferaVent。）形成层活动周期中用酶联免疫吸附法测定了形成层区域的内源IAA浓度的变化，并用自行发展的免疫荧光检测方法测定了由此处生活细胞制得的原生质体中IAA结合蛋白的分布。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。