原植体植物的

Chinaberry witches'broom, Ziziphus jujube witches'broom, Mulberry dwarf disease, JWB and Paulownia witches' broom phytoplasmas were identified by comparative studies of PCR amplify of phytoplasmal 16SrDNA、23rDNA and ribosomal protein gene, heteroduplex mobility assay, RFLP of PCR-amplified products (16S rDNA), cloning and sequence analysis of 16SrDNA and rp gene. An efficient procedure for rapid identification and differentiation of phytoplasmas was established, which could identify and differentiate phytoplasma collected from field.

通过对桑萎缩病、枣疯病、酸枣丛枝病、泡桐丛枝病和苦楝丛枝病植原体16SrDNA、23SrDNA和核糖体蛋白基因的PCR扩增、异源双链迁移率分析、16SrDNA PCR产物的限制性片段多态性分析以及植原体16SrDNA和rp基因的克隆和序列分析等比较研究，建立了一种快速确定未知植原体种类和分类地位的分子鉴别与鉴定优化程序；此程序可对田间采集的各种植物植原体样品直接进行快速鉴定和鉴别。

Using universal primers (R16mF2/Rl and R16F2/R2) for 16S rRNA and primers (rpF1/rpR1) for ribosomal protein gene to detect phytoplasmas associated with of chinaberry tree witches'-broom from Yunnan and Guizhou Province by polymerase chain reaction.

根据植原体16S rRNA基因通用引物序列(R16mF2/R16mRl、R16F2/R16R2)及核糖体蛋白基因通用引物序列(rpF1/rpR1)，应用PCR技术对采自不同省份的苦楝丛枝病植原体进行检测，结果从两个植物上分别获得苦楝丛枝病植原体云南株系和贵州株系。

Inoculating test plants through vector,observing symptom and using universal primers(R16mF2/R16mR1)for phytoplasma 16S rDNA were conducted.1.4 kb DNA fragments were amplified by polymerase chain reactionin DNA samples extracted from inoculated wheat and viruliferous Psammotettix striatus L.respectively.

采用介体接种植物，症状观察和应用植原体16S rDNA基因通用引物对R16mF2/R16mR1进行PCR扩增，在接种小麦和传毒介体中均扩增出1.4kb的特异片段，鉴定出小麦蓝矮植原体新寄主7种。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。