原核的

Objective:To provide the target antigen for the development of a ZP vaccine by using a prokaryotic expression system.

目的：通过原核表达系统获得研制ZP疫苗的靶抗原。

A prokaryotic expression vector pET28a-exchIL-2Rγ was constructed successfully and expressed, and the expressed protein was identified.

构建了鸡IL-2Rγ胞外区的原核重组表达载体，进行了表达和鉴定。

After lOmin delay only 81.2% pronucleus developed to blastula, significantly lower than control (93.2%).

滞留20min不引起原核胚、2-cell胚胎和桑椹胚以及囊胚的死亡。

We also successfully amplified Sry from pronucleus stage embryos, which prove that embryo can be sexed by Sry PCR method using one cell instead of the minimal detectable cell amount of 4 cells proved by others.

同时由于从原核时期扩增到了Sry，本文认为胚胎的性别鉴定取单个细胞即可。

Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

AbstractThough prokaryotic cells could hardly express recombinant human beta nerve growth factor with a proper threedimensional conformation, using of E.

基于原核表达系统极难表达具有正确三级结构的重组人β神经生长因子，E。

The aim of the study is to construct two vectors for efficient expression of soluble recombinant proteins.

本研究构建了两种高效表达可溶性重组蛋白的原核表达载体。

The result of ISH revealed that the mTSARG3 gene is expressed mainly in spermatocyte.

将PQE30／mTSARG3原核表达产物进行纯化，SDS—PAGE显示纯化后产物是分子量为36kD的蛋白。

Now many biologists still regard SSU rRNA tree as the standard tree of life.

沃斯首先采用SSU rRNA序列研究原核生物的系统发生关系。

Prokaryotic organisms sometimes considered a class or phylum or subkingdom; coextensive with the Cyanophyceae: cyanobacteria (blue-green algae).

有时被认为是一个纲或门的原核生物体；和蓝藻纲同域；蓝藻。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失