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For example, the eukaryotic protein expressed in E.

目前,虽有原核表达OPG的报道,但仍存在一些不足。

They appear to have a mixture of prokaryotic and eukaryotic genes.

它们被认为是某些真核生物基因和原核生物基因的混合体。

The study cloned the flic gene of App and performed sequencing and analysis of biological information. Polymerase chain reaction PCR was used to amplify the flic gene from.

构建猪传染性胸膜肺炎放线杆菌(Actionobacillus pleuropneumoniae, App)鞭毛蛋白编码基因的重组表达质粒,对其编码蛋白进行生物信息学分析,并将其在原核细胞中进行表达。

Mitochondria are self-replicating, and probably they are the evolutionary descendants of what were once free-living prokaryotes.

线粒体可以自我复制,很有可能是曾经游离原核生物演化而来的后代。

Objective To obtain high yield of recombinant human apolipoprotein A-I with normal structural and functional properties by a prokaryotic expression system.

目的 为获得高产量并具有正常结构和生物学活性的原核表达人载脂蛋白A-I。

We infer that hira gene is related to sperm nucleus decondensation in fishes.

在果蝇中的研究已经表明hira基因与精核解凝形成雄性原核有关。

In addition, the EO gene of hog cholera lapinized vaccine strain was

同时将兔化弱毒株EO基因分别置于原核与真核表达系中进行了表达,并对重组蛋白进行了初步的免疫学分析。

The isoform hMAM cDNA consisting of 270 bp, was different from the wild type one of 279 bp due to nine continuous base pair missing. Therefore the translational product of the isoform was seen to be lack of three continuous amino acid (-79~-81, VFM) compared with that of the wild type. The homology of the wild type and the isoform are testified to be 96% in both DNA and amino acid sequences.

乳腺癌组织和乳腺癌细胞株中发现两种hMAM cDNA亚型:hMAM和hMAM,长度分别为279和270bp,二者相差9个连续碱基,其翻译产物相差3个连续氨基酸残基(-79~-81,VFM),二者基因和氨基酸序列匹配度均为96%。cDNA片段分别插入原核表达质粒pGEX-KG,构建的质粒命名为pGEX-KG/hMAM和pGEX-KG/hMAM。

To obtain the African horse sickness virus VP7 protein, which is used to preparing serological diagnostic reagent and constructing AHS new generation vaccine, the prokaryotic expression is used to express VP7 protein.

为获得非洲马瘟病毒VP7蛋白,以用于制备AHS血清学诊断试剂并为AHS新型疫苗的构建奠定基础,笔者采用原核表达系统表达VP7蛋白。

The prokaryotic expression vector pET22b and host bacterium Roestta-gami are most suitable for CM-VEGF121,which solves the problem of low expression and insolubility.

原核表达载体pET22b和Rosetta-gami宿主菌为CM-VEGF121最合适的条件,解决了其表达量低和不可溶问题。

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