原核的

Thioredoxins, a ubiquitous heat-stable, acidulous small proteins with a redox activedisulfide bridge in its conserved motif -CPPC-, distribute universally in eukaryote andprokaryote and have a molecular mass of approximately 12Kda.

硫氧还蛋白是一类广泛存于原核生物和真核生物的热稳定、酸性小分子量蛋白质(其分子量约为12Kda)。

In addition, these two purified proteins could react with a specific antibody against mouse VEGF as expected. Conclusion Recombinant Xenopus laevis VEGF and its control mouse VEGF protein may provide tools for further study of anti-tumor active immunity with this xenogeneic protein vaccine in mouse tumor models.

重组非洲爪蟾和小鼠血管内皮细胞生长因子的原核表达、分离纯化及鉴定成功，为进一步研究异种VEGF蛋白质疫苗抗小鼠肿瘤模型奠定了基础。

First, nucleic acid sequence encoding ectodomain of AMAl was amplified from Pf genome and, was cloned into prokaryotic expression vector.

首先，自P.f基因组DNA中扩增出编码AMAl胞外域的基因片段，经测序确证后，克隆入原核表达载体。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果：单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合，与NIH3T3细胞无反应；1H3与原核表达的c-erbB2胞外区蛋白有特异性反应，而与无关蛋白KDR无反应。

As they are evenly dispersed throughout genomes, usually characterized as codominant and highly polymorphic systems amenable to genotyping by polymerase chain reaction, and generally inherited in a Mendelian fashion, microsatellites have become instrumental as genetic markers in many areas.

摘 要：微卫星标记广泛分布于原核生物和真核生物基因组中，具有密度大、多态性丰富、遵循孟德尔分离定律共显性遗传、易于PCR扩增等特点，已成为近年来最引人注目的新型DNA标记。

Study on Cloning the Gene of β-1, 3-glucanase in Tea Plant and Its Expression in Escherichia Coli

论文标题：茶树β-1，3-葡聚糖酶基因的克隆及原核表达分析

A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法，扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因，将其克隆到pKEN上，构建了该融合基因的原核表达载体pKENG-M。

Superoxide Dismutases are ubiquitously expressed antioxidant enzyme in aerobic organisms and catalyze dismutation of superoxide anion to hydrogen and molecular oxygen,the first step in active oxygen-scavenging systems.

超氧化物歧化酶(superoxide dismutase，SOD)在需氧原核生物和真核生物中广泛存在，是活性氧清除系统中第一个发挥作用的抗氧化酶。

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of4-Lys in order to provide a technique platform for application of recombinant enterokinase.

目的：制备能在原核表达系统中表达并特异性识别4-Lys序列的鼠源性肠激酶，为推广应用重组肠激酶提供技术平台。

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新