原核的

Thioredoxins, a ubiquitous heat-stable, acidulous small proteins with a redox activedisulfide bridge in its conserved motif -CPPC-, distribute universally in eukaryote andprokaryote and have a molecular mass of approximately 12Kda.

硫氧还蛋白是一类广泛存于原核生物和真核生物的热稳定、酸性小分子量蛋白质(其分子量约为12Kda)。

In addition, these two purified proteins could react with a specific antibody against mouse VEGF as expected. Conclusion Recombinant Xenopus laevis VEGF and its control mouse VEGF protein may provide tools for further study of anti-tumor active immunity with this xenogeneic protein vaccine in mouse tumor models.

重组非洲爪蟾和小鼠血管内皮细胞生长因子的原核表达、分离纯化及鉴定成功，为进一步研究异种VEGF蛋白质疫苗抗小鼠肿瘤模型奠定了基础。

First, nucleic acid sequence encoding ectodomain of AMAl was amplified from Pf genome and, was cloned into prokaryotic expression vector.

首先，自P.f基因组DNA中扩增出编码AMAl胞外域的基因片段，经测序确证后，克隆入原核表达载体。

Results:Monoclonal antibodies IH3,5A12,7E5,7F9 showed specific respo nse to NIH3T3-erbB2 cell and SKBR3 cell,no response to NIH3T3 cell.1H3 also boun d specifically to the extracellular domains of P185 protein that expressed by E.

结果：单克隆抗体1H3、5A12、7E5、7F9可与NIH3T3-erbB2细胞、SKBR3细胞特异性结合，与NIH3T3细胞无反应；1H3与原核表达的c-erbB2胞外区蛋白有特异性反应，而与无关蛋白KDR无反应。

As they are evenly dispersed throughout genomes, usually characterized as codominant and highly polymorphic systems amenable to genotyping by polymerase chain reaction, and generally inherited in a Mendelian fashion, microsatellites have become instrumental as genetic markers in many areas.

摘 要：微卫星标记广泛分布于原核生物和真核生物基因组中，具有密度大、多态性丰富、遵循孟德尔分离定律共显性遗传、易于PCR扩增等特点，已成为近年来最引人注目的新型DNA标记。

Study on Cloning the Gene of β-1, 3-glucanase in Tea Plant and Its Expression in Escherichia Coli

论文标题：茶树β-1，3-葡聚糖酶基因的克隆及原核表达分析

A fusion gene of green fluorescent proteinand movement protein of cucumber mosaic viruswas obtained by using recombination-PCR.

采用重组-PCR方法，扩增获得绿色荧光蛋白与黄瓜花叶病毒运动蛋白融合基因，将其克隆到pKEN上，构建了该融合基因的原核表达载体pKENG-M。

Superoxide Dismutases are ubiquitously expressed antioxidant enzyme in aerobic organisms and catalyze dismutation of superoxide anion to hydrogen and molecular oxygen,the first step in active oxygen-scavenging systems.

超氧化物歧化酶(superoxide dismutase，SOD)在需氧原核生物和真核生物中广泛存在，是活性氧清除系统中第一个发挥作用的抗氧化酶。

Objective To establish a prokaryotic expression system for expressing mouse enterokinase which could recognize and cut the sequence of4-Lys in order to provide a technique platform for application of recombinant enterokinase.

目的：制备能在原核表达系统中表达并特异性识别4-Lys序列的鼠源性肠激酶，为推广应用重组肠激酶提供技术平台。

Recombinant mouse enterokinase can be expressed in Ecoli in inclusion manner so that the prokaryotic expression system for recombinant enterokinase with native conformation needs to be optimized.

鼠源性肠激酶轻链在原核细胞中多以包涵体形式表达，天然构象重组肠激酶的获得还需对表达系统进行优化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失