原核的

Producing PHAs,and then were orientationally inserted into a prokaryotic expression vector pBV220. By analyzing the bioactibity and function of their products, the biological function of phaA, phaB and phaC was confirmed. These have been done in our laboratory. To make better use of the three genes by plant bioreactor, two groups of different plant expression vectors that can be used to produce PHAs in different plants were constructed, and the transformation of PHA in Oxalis triangnlaris has been preliminarily reseached.The following is the main results:Three plant expression vectors pBI121-A,pBI121-B and ubquiting-C, which can be used to be transformed into Oxalis triangularis were respectively constructed by using the phaA, phaB and phaC.

菌株的亚克隆基因组片段中，分离出phaA、phaB和phaC三个基因片段，定向克隆至原核表达载体pBV220，通过对其生物活性与功能分析，确证了基因phaA、phaB和phaC的生物学功能；为了通过植物生物反应器对这三个基因进一步研究利用，本实验利用这三个基因构建了两组可用于不同植物生产PHA的真核表达载体，并对紫叶酢浆草进行PHA基因遗传转化的初步研究，获得主要结果如下：利用克隆的phaA、phaB、phaC三个基因分别构建了可用于转化紫叶酢浆草表达载体pBI121-A、pBI121-B、uBquiting-C；可用于玉米转化的种子特异性表达载体p7S-A、pBI121-S-B、uBquiting-C。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用实验一确定的PCR条件，以Hprt为阳性对照，用巢式RT-PCR对小鼠早期胚胎进行Xist基因的转录分析，结果发现，转录Sry基因的睾丸组织以及雄性胚胎，从受精卵发育到囊胚的过程中，基本上不转录Xist基因；不转录Sry基因的雌性卵母细胞和雌性胚胎，从出现原核开始，到发育至2-细胞期的过程中，Xist基因一直不转录，但是，从4-细胞期开始，一直到孵化前囊胚阶段，雌性胚胎都转录Xist基因。

The relationship between the proterozoic Era and explosive evolution of the life on the Cambrian period was preliminary explored by an Ecological and Evolutionary approach in this paper.

距今38亿年前地球最早生命诞生以来，至元古代早期第一个真核细胞开始出现；从无性分裂或出芽繁殖的原核生物到有性繁殖的真核单细胞生物以及从单细胞的原生动物到真核多细胞的后生动物的进化，无疑是生命进化过程中非常重要的事件。

Protein folding, transportation orientation. When the body is stressed, the synthesis of Hsp60 can be markedly induced to stabilize and refold proteins. Hsp60 is benefited for the function of heart as it is expressed in the different time of embryo. 3. MAPKKK4 or MEKK4 is one of the members of the major systems participating in the cellular signal transduction of MAPK.

1、在小鼠胚胎的发育中，胚胎心脏的分化和功能的完善需要大量的蛋白质参与。2、Hsp60 是一个分子量为 60kDa 的蛋白质，普遍存在于原核细胞和真核细胞中，主要存在于细胞质和线粒体基质中，其一是起分子伴侣的作用，即对新生蛋白质进行折叠、转运和定位。

We reconstructed the gene of human scFv and prepared scFv and scFv antibody fragments by prokaryotic expression system PET22b/BL21(DE3). After being purification by Ni-NTA column and renaturalization by urea dialyzation, the affinity and thermostability of scFv and scFv were detected.

结果表明，成功构建了scFv基因；实现了scFv和scFv在原核中的高效表达，二者的表达量均占菌体总量的35％以上，且成功的纯化了表达的重组蛋白，复性后获得了有活性的纯化重组蛋白。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本实验主要利用卵母细胞的乙醇激活、胚胎体外培养和免疫荧光细胞化学方法对小鼠卵母细胞乙醇激活效率、影响因素、孤雌胚类型、发育、原核形成、迁移及早期胚胎卵裂过程中细胞骨架的组装、作用等进行了系统的研究和分析，结果显示：(1)小鼠卵母细胞的乙醇激活结果表明，随着乙醇浓度的升高和作用时间的延长，卵母细胞激活率和发育率都显著提高，但乙醇浓度过高和作用时间过长会导致卵母细胞碎裂率的显著增加，7%乙醇作用卵母细胞7min 为最佳激活条件。

The purpose of this test is to reveal the mutagenic potential of a substance in a prokaryote organism and whether the reactive metabolite is a product of metabolic activation by mammalian enzymes.

这次试验的目的是揭示在原核微生物的诱变物质的潜力和活性代谢物是否是一种代谢酶的激活产品的哺乳动物。

By a two-promoter vector, we successfully coexpressed DsbA-DsbC fusion protein with hNGF in procaryote cytoplasm and obtained the soluble hNGF protein, but the DsbA or DsbC alone do not show this function.

通过本课题的研究，我们根据DsbA和DsbA~蛋白的性质特点获得了一种原核通用融合表达载体，该载体在表达真核来源的活性蛋白或细胞因子具有促可溶和保持目的蛋白天然活性的功能。

The scientists will transfer the pro-nuclei -- the components of a human embryo nucleus -- made by one man and woman into an unfertilised egg from another woman.

科学家们将把取自于一男一女受精卵的原核（pronuclei，人类胚胎细胞核的构成成分）移植到另外一名妇女提供的一个未受精的卵子中去。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。