原核的

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

A dominating antigenic region located at the amino-terminus of Hantaan virus nucleocapsid protein.These recombinant truncated proteins can be used to diagnose the infection of HFRSV serologically.

汉滩病毒核蛋白的氨基端含有一主要抗原决定簇区；原核表达的截短核蛋白在HFRS的血清学诊断中具有一定的价值。

After being induced to differentiate in vitro, cells with various morphologies showed β-gal activity, including nerve cells, neuroglial cells, epithelial cells. We also detected β-gal activity in a wide variety of tissue elements in solid tumors made by injecting the MC15 and MA34 cells into syngenic mice. Then we produced chimeric embryos by injecting the MC15 cells into Kunming blastocysts and recovering the embryos at various developmental stages.

SiCMVIE(Simian Cytomegalovirus Immediately Early)启动子是一个强启动子，本文首次将SCMVIE启动子应用到ES细胞途径上制作嵌合鼠，并通过lacZ基因在ES细胞和早期嵌合胚胎中的表达，表明该启动子是一个能在小鼠多数组织表达的启动子，这与1993年Peppel等人用原核显微注射法获得的转基因小鼠上所得的有关结果是基本上是一致的。

A 1: Uninduced supernant of cell lysate by sonication; 2: Induced supernant of cell lysate by sonication; 3: Uninduced precipitate of cell lysate by sonication; 4: Induced inclusion body of cell lysate by sonication; 5: Purified ULBP4 protein; M: Low molecular marker proteins.

2.3 体外NKG2D结合实验和细胞因子分泌实验间接ELISA检测结果显示：原核表达复性后的ULBP4能与人NKG2D结合，而带His标签的无关蛋白则不能与之结合，说明复性后的ULBP4仍能维持正确的天然构像。

In order to test the antibodies to E6 and E7 induced by the recombinant vaccina virus, the fusion proteins of GST and E6 or E7 were expressed in E. coli using pGEX-2T vector. The expressed proteins were confirmed by Western-blot and purified by glutathione sepharose CL-4B affinity chromatography. The purified proteins were used to detect the anti-E6 and-E7 by ELISA.

为便于检测重组痘苗病毒所诱发的E6和E7抗体，本文利用原核表达载体pGEX-2T，在大肠杆菌中分别高效表达了GST与E6或E7的融合蛋白，并利用纯化的融合蛋白成功地建立了E6和E7抗体的ELISA检测方法。

Based on the conclusion of determining the concentration of rChIFN-γprokaryotic expression and titrating antiviral of sf9 cells, to establish the quantitive ELISA for ChIFN-γ.

在测定原核表达rChIFN-γ的浓度和滴定昆虫细胞表达rChIFN-γ的抗病毒的活性的基础上，建立了鸡γ-干扰素的定量ELISA检测方法。

Rabbit zygotes were cultured in sequence culture system that contains: R1 culture medium which is with low concentration of glucose (1.0 mmol/L) and then R2 culture medium which is with high concentration of glucose (3.5 mmol/L).

试验采用含低浓度葡萄糖(1.0mmol/L)的R1培养液和含高浓度葡萄糖(3.5mmol/L)的R2培养液对兔原核期受精卵进行了体外序贯培养，并与传统的RID单一培养进行比较，研究了兔早期胚胎在体外序贯培养系统中的发育效果。

The diversity of liquor-making microbes in China has formed into a treasury of biological resource. The microbial species mainly include prokaryote such as bacteria and actinomyceto, and eucaryote such as yeast and mould.

中国白酒酿造微生物的多样性是生物资源的宝库，其种类主要包括原核生物中的细菌、放线菌，真核生物中的酵母菌、霉菌。

Comparison of the coding regions of BH1-BH4 genes showed that BH1 had a lower sequence identity to other previously published B-hordeins than the other three B-hordeins obtained in this study. BH1 was then cloned in a bacterial expression vector based on bacteriophage T7 RNA polymerase. The resulting plasmid produced a 28.15 kDa protein in Escherichia coli. The potential value of B-hordein genes in grain quality improvement of hull-less barley has been discussed.

并且，在4个基因BH1～BH4中，BH1与先前报道的B组醇溶蛋白基因有较低的序列相似性，因此我们对BH1基因进行了原核表达，含该基因的表达载体在大肠杆菌中表达出相对分子质量为28.15 kDa并以包涵体形式存在的蛋白，进一步对其在青稞谷粒品质改良中的潜在价值进行了探讨。

The second part of the paper introduces the Z curve methodology which is the basic tool in analyzing prokaryotic genomes and gives a brief introduction to the application of Z curve method in various areas such as gene finding in prokaryotic genomes, isochore structure in eukaryotic genomes and identification of replication origins in the genome of archaeon and bacteria.

论文的第二部分介绍了DNA序列的Z曲线方法，这是我们分析原核生物基因组的主要工具，同时简单介绍了Z曲线方法在基因识别，基因组的isochore结构以及在细菌和古细菌复制起始位点识别等领域的应用。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。