原核的

Form、structure、characterization and function of prokaryotic、eukaryotic and molecular organisms;②nutrition and cultivation techniques of microorganisms;③physiological and metabolizable production of microorganisms;③theory of genetic variation and microbic breeding technique of microorganisms;⑤ecology and environmental protection of microorganisms;⑥Infection、 Immunity and biologics of microorganisms;⑦classification and identification of microorganisms and the international Code of Nomenclature of microorganisms.and microbial high-techs developing on these as the fundament of life sciences.

主要讲授：①原核微生物、真核微生物及分子生物的形态、结构、特点和功能；②微生物的营养和培养技术；③微生物生理及代谢产物；④微生物遗传变异理论和育种技术；⑤微生物生态和环境保护；⑥微生物传染、免疫和生物制品；⑦微生物分类鉴定和国际命名法则，以及在此基础理论上发展起来的微生物高新技术，是当代生命科学研究的基础。

Sequence analysis showed that the size of 16S rRNA gene was in 1235 bp, and lacking several regions which regarded as eukaryotic sequence, was more similar with prokaryotes than eukaryotes in sequence structural feature; it could be implied that the microsporidia had split from ancient eukaryotes very early in time on the phylogenetic tree.

序列分析表明，16SrRNA基因大小为1235bp，缺乏多个被认为是真核生物序列的区域，序列结构特征更多地类似于原核生物的，在进化上，推测微孢子虫在很早以前与原始真核生物产生分歧；分子进化分析发现，同属的微孢子虫可以处于系统进化树不同的分枝。

Myxobacteria have by far the largest prokaryotic genome and the most abundant secondary metabolites gene clusters. Our Myxobacteria genomics projects will help us understand their value in-depth.

粘细菌具有迄今为止最大的原核生物基因组、最丰富的生物活性次级代谢产物基因群，相关基因组计划的启动与实施将为我们深入研究生物进化、阐明粘细菌分化发育及其社会学行为、开发重要功能基因起到重要的推动作用。

Cumulus-enclosed oocytes from follicles in prepubertal gilt ovaries from alocal abattoir were cultured in modified TCM199 with and without 10 IU/ml PMSGand hCG for 22 hours respectively.After fixation and dying in 1% acid orcein,itshowed that 77.6% oocytes reached MII stage.Maturated oocytes were denudedwith 1mg/ml hyaluronidase,and fertilized with fresh or thawed sperms in modifiedTBM for 6 hours,then cultured in NCSU23 for 14 hours.The fertilization rates were87.5% for fresh sperms,64.1% for thawed sperms.

抽取从屠宰场获取的猪卵巢中的卵母细胞，在加入和不加hCG和PMSG的mTCMl99培养液中分别培养22小时，地衣红染色发现77.6％的卵母细胞达到MII期；去卵丘细胞后在mTBM中用鲜精或冻精解冻后受精6小时，在NCSU23中培养14小时后固定染色发现受精率分别为87.5％和64.1％；电激活后在NCSU23中培养20小时后固定染色发现原核形成率为94.1％。

Four major OTUs of prokarotype X group belong to Cyanobacterium.

原核类群X的4个序列主要属于蓝细菌类群，其中JS－X2与在美国黄石公园温泉发现的Uncultured Cyanobacterium (L35331)有95％的相似性，并且与已经全基因组测序的嗜热蓝细菌聚球藻Thermosynechococcus elongatus BP-1 (47118315)有89％的相似性。

The purpose of this course is to study the microbiological science and to give students the basic knowledge further study of phytopathogenic microbiology. Course contents include the original of microbiology, chemical principles, microscopy and staining, morphology、structure and function of prokaryotic

本课程之目的，在使学生了解微生物的起源，化学的基本原理，显微镜与染色，原核生物与真核生物细胞之形态、构造及功能，微生物的生长与代谢，遗传与生物技术及微生物的防治，以增进学生未来在研究植物病原微生物之相关基础。

Results The Southern blot result showed that 4 among 188 goats produced by pronuclear microinjection had integrated hEPO gene.

结果在原核注射获得的188头羔羊中，经Southern blot法检测有4头羊含有hEPO基因，其中3头为母羊，1头公羊于出生后20d死亡；3头转基因母羊hEPO基因的拷贝数分别为1、10、2；Western blot检测结果显示转基因羊乳中的hEPO分子质量为32kDa；MTT法检测结果表明，在泌乳10d的3只转基因羊乳汁中，每毫升乳汁中hEPO活性分别达到1.17×102IU、1.90×104IU、1.91×104IU。

The present study was conducted to investigate the effect of 6-DMAP on parthenogenetic activation rate, pronuclear formation and in vitro parthenote development of in vitro matured goat oocytes.

体外成熟的山羊卵泡卵母细胞用7%的乙醇和5μmol/L的离子霉素刺激处理后，分别置于含2 mmol/L 6-二甲基氨基嘌呤(6-DMAP)和不含6-DMAP的培养液中培养4 h，研究6-DMAP对体外成熟山羊卵泡卵母细胞孤雌激活率、原核形成以及孤雌胚体外发育潜能的影响。

Intracytoplasmic sperm injection is a very important assistant reproductive technology for treatment of man infertility and fundamental research , Success in ICSI in human and other animals has recently been achieved., The present pilot studies were conducted to establish a preliminary procedure for ICSI in mice, explore the experimental conditions of ICSI in mice, investigate factors influencing the test results, and accumulate technologic basis for the next studies on pronuclear exchange in mice .

本部分研究论文初步建立了小鼠ICSI技术的基本操作程序，摸索了小鼠ICSI研究的操作条件，总结了一些影响显微注射效果的重要因素，并为下一部分小鼠原核置换的研究奠定了雄厚的技术基础。

The healthy survivals were also transferred into the oviducts of pseudopregnant female mice. PCR was used to analyze the integration of the transgene in the genomes of mice.

选用内交系C57BL／6J种小鼠作为受精卵的供体鼠，昆明种小鼠作为假孕母鼠，将长2583bp、携带有CMV启动子和人CKLF-1基因的片段显微注射到受精卵的原核内，然后将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。