原核的

our successful cloning and expression of human tum 5 gene and purification of rhtum 5 protein lay a basis for further study on the application of this protein to anti angiogenesis in vitro and in vivo.

成功的构建了人tum 5基因的原核表达载体，并纯化了该基因的原核表达产物。

The cloned Buchnera groEL genes of Schizaphis graminum and Rhopalosiphum maize were ligated into pBV221,pET30a and pTrcHisA expression vector for construction of pBVRM,pBVSG, pETRM,pETSGPR,pTrcRM and pTrcSG. They were transformated into E. coli BL21(DE3),DH5a and JM109 for studying their expression.

2将克隆的玉米蚜和麦二叉蚜Buchnera groEL基因连接到原核表达载体pBV221、pET30a和pTrcHisA上，构建了含2个目的基因的6个表达原核表达载体：pBVRM、pBVSG，pETRM、pETSG和pTrcRM、pTrcSG。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

We concluded that the Spidroin2 cDNA of Nephila clavipes can be expressed in prokaryotic expression system, and addition of exogenous alanine plays important role in the promotion of expression of the naive gene of spider silk protein.

本研究证实，蜘蛛牵引丝蛋白Spidroin2 cDNA可在原核细胞内正确表达，外源丙氨酸的加入对于提高天然蜘蛛丝蛋白基因在原核系统的表达作用明显。

The extra pronuclear of triploid zygotes after IVF is from sperm and the zygotes have no implantation value.

IVF后3PN卵子中多余的原核多为精子来源，没有移植价值；ICSI后3PN中多余的原核非精子来源，ICSI后3PN中二倍体率与IVF相比显著增高，在患者ICSI后无2PN卵子时可考虑行植入前遗传学诊断。

In this study, a new horizontal microinjection system has been established to solve these problems. Because the average time per pronuclear embryo by microinjection has been significantly shortened with the horizontal system, the cleavage rate and 16-cell rate of those microinjected embryos increased markedly.

为了解决这些问题，本研究有针对性地设计了新型的水平微注射系统，利用此系统，可明显缩短常规注射原核胚的平均时间，注射后原核胚的分裂率、8-16细胞胚胎发育率也明显提高。

Methods: Total RNA was extracted from esophageal carcinoma tissue, the primer containing special enzyme was designed, NY-ESO-1 fragment was amplified by RT-PCR, and then was cloned into the expression vector pET-15b by LP Recco PCR Cloning. The recombinants plasmid pET-15b-NY-ESO-1 were identified by restriction endonucleases digest analysis and sequence analysis.

从人新鲜的食管癌组织中提取总RNA，通过RT-PCR技术扩增NY-ES0-1基因3'端的340bp片段，采用靶向克隆法将目的基因插入到原核表达载体pET-15b中，得到重组表达质粒pET-15b-NY-ESO-1，经过筛选，挑选出阳性克隆进行XhoⅠ和BamHⅠ双酶切图谱分析、PCR检测和扩增产物核苷酸序列分析等，鉴定所构建的原核表达载体。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

Early cleavage rate,embryo quality,the rate of blastocyst,implantation and pregnant rates from these zygotes were observed,and the transferred embryos were selected according to the pronuclei orientation and early cleavage time.

根据受精卵原核构型的不同分为四组，结合构型类型及早期卵裂与否进行选择性移植，通过观察优质胚胎率、囊胚形成率、种植率和怀孕率，来评估合子原核构型和早期卵裂与胚胎发育潜力的关系。

FtsZ, a functional protein existed in prokaryotes and plants controlled the division of prokaryotic cells and plastids in plants.

FtsZ是广泛存在于原核生物和植物中的一种功能蛋白，它控制着原核细胞和质体的分裂过程。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失