原核的

With regard to the rapidly increasing sequence data of prokaryotic genomes, accurate gene identifications and genome annotations have become more and more important.

近年来原核生物的基因体定序速度越来越快，相对地基因的鉴定以及基因的注解也变的越来越重要。

A double-antibody sandwich ELISAemploying monoclonal antibody 5G5 was developed for detection of P27 antigen of avian leucosis virus.The limit of viral antigens detected was 5?

利用原核表达的禽白血病病毒P27蛋白作为抗原制备的单抗作为包被抗体，以制的酶标兔抗P27作为酶标抗体，在国内首次建立了检测ALV抗原的双抗体夹心ELISA方法。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞，获得重组杆状病毒rBV-σC；SDS-PAGE和Western-blot分析表明，在sf-9细胞中表达了分子质量约37ku的σC蛋白，该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应，这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

The baculovirus expression system, utilizing the strong polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus , has become an important tool for protein production in the field of molecular biology.

由于核型多角体杆状病毒的多角体蛋白不是病毒复制必需的蛋白，利用转录多角体蛋白基因的强启动子可以高效表达外源蛋白，包括原核和真核基因编码的蛋白。

PCR-SSCP (single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed:(1) The pronucleus microorganism's community was similar and also had the polymorphism in each simple of various fermentation stage;(2) Different microflora had complex ecology effects of coordination and the restriction;(3) The diversity indexes of different stage of Daqu microorganisms were around 1.69~2.01, and the composition of them was stable relatively;(4) The similarity indexes were 0.67~1.00, and much higher in the approaching stages.

采用PCR-SSCP技术对浓香型大曲发酵过程中原核微生物群落结构的变化情况进行了研究，结果表明：(1)发酵各时期曲样的群落结构相似，同时也具有多态性；(2)不同微生物群落具有协同和制约的复杂生态学效应；(3)各时期曲样微生物多样性指数均在1.69~2.01之间，群落结构相对较稳定；(4)各曲样微生物群落相似性位于0.67~1.00之间，邻近时期样品间的相似性程度较高。

The purpose of the experiment is to realize the prokaryotic expression of β-glucosidase gene from Acetobacter xylinum ATCC 23769, which will be used to produce enzyme preparation so as to develop the efficiency of extracting resveratrol from plants. Aspergillus niger β-glucosidase gene is cloned both at DNA lever and cDNA lever, which will lay a foundation for further research about its expression in Saccharomyces cerevisiae in a secretory form with the aim of increasing resveratrol contents in wines.

本实验实现了木醋杆菌β-葡萄糖苷酶基因的原核表达，目的是为了生产粗酶制剂，用于提高从植物中提取白藜芦醇的得率；分别在DNA和cDNA水平从黑曲霉中克隆β-葡萄糖苷酶基因，为下一步在酿酒酵母中实现分泌表达以提高葡萄酒的白藜芦醇含量的研究奠定基础。

Transgenic plants expressing GNA showed significant insecticidal activity towards \\omoptera insects such as aphids and brown planthopper in bioassay and feeding tests.In this paper a lectin gene was firstly isolated from leaves of Zephyranthes grandiflora, belonging to Amaryllidaceae, by the use of rapid amplification of cDNA ends technique. Characterization, functional prediction and construction of plant and prokaryotic expression vector of this novel gene were studied.

本实验采用cDNA末端快速扩增(Rapid amplification of eDNA ends，RACE)技术，首次从中国特有的野生植物韭莲(又名风雨花，Zephyranthes grandiflora)的叶片中分离克隆出了一个新的凝集素基因，对基因进行了序列分析和功能预测，并成功地构建了韭莲凝集素基因的植物表达载体和原核表达载体。

We gained the Buchnera groEL gene of Schizaphis graminum, and Rhopalosiphummaize biotype through this study , and have done a primary study of expression. Based on this study, we can further probe the role during aphid transmission of Buchnera GroEL and utilize them through the ways of genetic engineering.

蚜虫内共生菌Buchnera groEL基因的获得、初步的原核表达研究以及GroEL蛋白抗血清的制备，为进一步研究GroEL蛋白在蚜虫传毒中的作用方式和位点以及在基因工程途径的利用打下了基础。

ASES is 59% identical to Artemisia cyclase cDNA clone cASC125, 50% identical to epi-cedrol synthase from A. annua, 48% identical to amorpha-4, 11-diene synthase from A. Annua, 39% identical to the 5-epi-aristolechene synthase from tabacco, 38% identical to vetispiradiene synthase from H. Muticus, 41% identical to the δ-cadinene synthase from cotton. The coding region of cDNA was cloned into procaryotic expression vector pET30a and overexpressed in E. coli BL21 (DE3). The cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction. The tissue specific expression patterns were analyzed by RT/PCR.

克隆的倍半萜合酶氨基酸序列与烟草马兜铃烯合酶、莨菪岩兰螺旋二烯合酶、棉花杜松烯合酶的一致性分别为39％，38％和41％；与青蒿柏木脑合酶、紫穗槐二烯合酶和一个推测的倍半萜合酶克隆cASC125的一致性为50％，48％和59％。cDNA编码区序列被克隆进原核表达载体pET-30a，并在大肠杆菌BL21（DE3）中诱导表达，但过量表达的蛋白主要是以不溶性蛋白形式存在。

The study on the expression of TGF β1 gene in Escherichia coli, not only can provide great amounts of TGF β1 for its clinical and basic research, and also will bring some new ideas and means for the research of other dimeric growth factors.

开展TGFβ1基因在大肠杆菌中表达的研究，不仅有可能获得大量具有生物学活性的TGFβ1产品，从而为TGFβl深入的基础和临床研究提供可能；而且可能为其他双体生长因子的原核表达提供新的思路和方法。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。