原核的

METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要：通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt；采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中，然后将注射存活的受精卵植入假孕母鼠的输卵管；取其产3 wk龄子代鼠进行PCR初选，再通过Southern杂交进一步确证；利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

The effect of the specific inhibitor cibacron blue of GSTs and antiparasitic drugs albendazole, praziquantel and artesunate on the activities of recombinant GSTM, GST1 and GST2 was evaluated.

研究目的解析三个胞质GSTs的基因结构及其特点，克隆三个胞质GSTs基因，鉴定原核表达的重组GSTs的免疫反应性、免疫原性、催化活性及其免疫定位和抑制剂、常用抗寄生虫药对催化活性的影响。

The cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology.In the experiment, the full code sequence of bar gene was cloned by PCR from transgenic herbicide resistant Bobwhite wheat and checked. It was expressed in E.coli and its protein was determined.

本实验从抗除草剂转基因Bobwhite小麦中，利用PCR克隆的方法扩增出bar基因全长，并在原核表达系统中表达，鉴定表达蛋白的活性，将能够正确编码PPT乙酰转移酶的bar基因片段，经过适当的修饰构建入真核表达载体。

Because the dimeric form is the active form to exert its biological activities, many trials failed to express TGF β s in prokaryotic systems because of their complex folding manner and disulfide patterns. In 1991 Cerletti and coworkers from Ciba-Geigy Corporation in Switzerland successfully finished the functional expression of human TGF β 2 in bacteria, but the details are protected by the patent.

由于双体是其发挥生物活性的形式，TGF β复杂的折叠方式和二硫键配对使其早期原核基因工程研究都以失败而告终。1991年，瑞士Ciba-Geigy公司的Cerletti等成功完成了人TGFβ2基因的细菌表达，但其技术细节被专利加以保护。

By means of polymerase chain reactionand DNA recombinant techniques,the gene encoding mature ouse IL-4 was obtained from plasmid pCD-mIL4 and was inserted into expressionvector pBV220,which contains _RP_L double promoters,synthesized SD sequence and temperaturesensitive CIts857 gene,thus an expression lone eferred as pBV-mIL4 was constructed.

该质粒含有 P_RP_L 串联启动子，合成 SD 序列和温度敏感的CIts857基因，并且去除了完整小鼠 IL—4 cDNA 中3′侧翼非编码区，以原核系统高频使用的强终止密码子TAA 代替了原小鼠 IL-4基因的终止密码子 TAG，经 SDS-PAGE 及凝胶密度扫描分析，表达的重组小鼠 IL-4分子量约为14.2KDa，占总菌体蛋白量的25～30%。

Results: To directly examine the role of IL-10 in allergen-induced pulmonary inflammation, we analyzed cellular components in the bronchoalveolar lavages of each group.

我们构建了 hIL八 0的原核表达载体 pBADlhIL—10，并且发现外源性rhIL—10可以抑制小鼠支气管肺泡灌洗液中 IL—5的产生，这可能是由于 IL—10具有抑制炎症细胞在炎症局部浸润的特性。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核，大多能够逐渐解凝、液化并膨大，最终形成形态正常的雄性原核，但在第一次卵裂早期退化成浓缩的染色质小体，并在胞质分裂时随机地分配到其中一个子细胞里的分裂沟附近。

Expression vectors pET-TPO and pBV-TPO were constructed to express full-length TPO cDNA in E.coli . Influence of SD-AUG and promotors on the expression level was studied.

在克隆并分析了TPO cDNA的基础上，将全长hTPO cDNA克隆到原核表达载体pET-15b和pBV220中得到了表达，并且以pBV220为载体探讨了SD序列与AUG之间的距离对cDNA表达效率的影响；为了进一步提高表达效率，将hTPO cDNA 5？

The organization of the ribosomal RNA genes of N.antheraeae is LSU-ITS1-SSU-ITS2-5S,a pattern similar to those of N.bombycis.

柞蚕微孢子虫的核糖体基因排列顺序为LSU—ITS—SSU—ITS—5S，和家蚕微孢子虫的排列顺序相同，是一种即不同于真核生物，也不同于原核生物的排列顺序。

In order to study the expression, subcellular distribution and the function of NS2 protein, the coding region of NS2 was amplified from the hindgut tissue of cockroaches infected with PfDNV by RT-PCR and then the recombinant prokaryotic expression vector pET28a-NS2 was constructed. The recombinant plasmid was transformed into E. coli BL21 (DE3) to express the 6×His fusion protein in the bacteria.

为了对该基因进行深入的功能研究，从感染了黑胸大蠊浓核病毒的蟑螂的后肠组织中通过RT-PCR得到ns2基因编码序列，将其构建于原核表达载体pET-28a，转化大肠杆菌BL21(DE3)获得融合表达产物。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。