原核的

The relationship between cytoplasm and nucleus is the basic problem of biology, and nuclear transfer and pronuclear transplantation are the important methods to research their relations.

细胞核与细胞质的关系是生物学的基本问题，核移植和原核置换是研究核质关系的重要方法。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

The successful cloning and expression of yak IL2 gene is putatively useful for the development of novel immunopotentiator to raise the immunity of yak against various infectious pathogens and the immuno-protective efficacy of vaccinations.

本实验成功克隆了犛牛IL2基因，其原核表达产物具有显著的免疫活性，这为研制新型免疫增强剂来提高犛牛对各种疾病的抵抗力，增强犛牛疫苗的免疫保护率奠定了基础。

XC7201 is an interesting protein comprising 176 amino acids. Many sequences in the prokaryote and eukaryote kingdom with high similarity score were detected by BLAST search. It was predicted to a 3-hydroxyanthranilate 3,4-dioxygenase (3HAO), which is an extradiol dioxygenase that catalyzes the final conversion step of tryptophan to quinolinic acid in the kynurenine pathway.

XC7201由176个胺基酸所组成，比对已知物种资料库后发现在原核真核菌中都有类似的序列，其功能被预测为3-hydroxyanthranilate 3，4-dioxygenase (3HAO)。3HAO是一种双氧化酵素，在生物体进行把Tryptophan转变成Quinolinic acid的生合成途径中最后一个反应的催化。

Inclusion bodies are easily formed when recombinant proteins are expressed in E.coli system, and how to renature these inclusion bodies is now becoming the key problem in the genetic engineering.

E.coli作为目前应用最为广泛的原核表达系统，在异体表达蛋白质的过程中容易形成无活性的包涵体。

Using Perl as programming language and integrating databases technology, microsatellites, longer than 12 nucleotides in length, in 29 eukaryotic and 1180 prokaryotic genomes were computed and censused in great scale.

本研究采用Perl为编程语言结合数据库技术等方法，对29个真核生物基因组和1180个原核生物基因组的DNA序列上长度超过12的微卫星进行了大规模的计算和统计分析。

In order to express he Opening Reading Frame fragment of Banana bunchy top virus Hainan isolateDNA2 correctly ,a pair of primers were designed in accordance with the reported BBTV-HN DNA2 gene sequence.

本文根据已报道的香蕉束顶病毒海南分离物(Banana bunchy top virus BBTV-HN)BBTV DNA2的基因序列，设计一对引物，以海南地区表现束顶病症状的香蕉组织总DNA为模板，利用PCR方法克隆了DNA2编码区，将其插入到原核表达载体pGEX-6p-1谷胱苷肽-S-转移酶基因下游。

In eucaryotes, the ribosomes in the cytoplasm are unaffected by chloramphenicol, but those in the mitochondria, being prokaryotic in character, are attacked.

在真核生物中，在细胞质中的核糖体不受这种抗生素影响，而在线粒体中的具有原核性的核糖体却会受到攻击。

Protein phosphorylation catalysed by protein kinase is the predominant form of signaling in both procaryotes and eucaryotes, Protein Ser/Thr and Tyr kinase play essential roles in signal transduction.

蛋白质激酶催化的蛋白质磷酸化是原核和真核生物中一种主要的信号传导方式，蛋白质Ser／Thr和Tyr激酶在信号传导过程中起着必需的作用。1993年，张承才在Anabaena sp。

MAIN OUTCOME MEASURES: Construction and purification of Znf430 prokaryotic expression vector as well as its location in the cytoplasm of eucaryotic cells.

主要观察指标：锌指蛋白 403的原核表达载体的构建、纯化及在真核细胞细胞质中定位的结果。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。