原核的

What are the most significant differences between the organization and expression of prokaryotic genes and eukaryotic genes?

原核生物的基因与真核生物的基因在组织形式和表达方式方面有哪些主要的区别？

Objective To amplify the hexokinase gene of Plasmodium falciparum isolate FCC1/HN, compare the sequence with that of other P. falciparum isolates and human, and construct its prokaryotic and eukaryotic expression plasmids.

目的 扩增恶性疟原虫FCC1／HN株的己糖激酶编码基因，构建其原核和真核表达重组质粒，测定其序列，比较它及它推导的蛋白质与恶性疟原虫其他株和人之间的差异。

Anti-histidine-tag antibody is usually used for identification of expression of the recombinant protein expressed using pRSET-A. A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence.

质粒pRSET-A是一个常用的高效原核表达载体，编码一N端含组氨酸标签（6×His）的34aa前导肽序列，以方便利用抗组氨酸标签抗体鉴定或纯化所表达的重组蛋白。

The efficiency of transgenic animal production by the traditional method ofpronuclear microinjection is very low.

以传统的原核显微注射法生产转基因动物的效率十分低下，严重地制约了转基因动物研究的进一步发展。

It is found in all photosynthetic cells of plants and protoctists but not in photosynthetic prokaryotes.

存在于所有的植物和原生生物的光合细胞中，但在能进行光能合成的原核生物中却不存在。

Horizontal gene transfer is an important evolutionary mechanism in prokaryotic genome evolution and has been widely studied.

在原核生物的进化过程中，基因的横向转移是一个重要的进化机制。

Second, some of these inhibitors affect prokaryotic but not eukaryotic protein synthesis and thus are medically important antibiotics.

第二，有些这些抑制剂的影响原核，而不是真核细胞的蛋白质合成，因此是医学上的重要抗生素。

Then the linear vector was microinjected into the pronucleus of the KM×B6D2F1 zygotes to produce the transgenic mice. Thirty-four adult mice were produced from 286 of injected embryos.

用显微注射方法，将线性化的载体DNA注入KM ×B6D2F1小鼠受精卵的的原核内部，然后将胚胎移植到输卵管内，13只受体怀孕产仔。

Positive and negative regulation In negative regulation of prokaryotic gene expression,bound repressor prevents transcription of the structural genes.

正调控和负调控在原核生物基因表达的负调控中，与阻遏物的结合能够阻止基因的转录。

Any of the unicellular, prokaryotic microorganisms of the class Schizomycetes, which vary in terms of morphology, oxygen and nutritional requirements, and motility, and may be free-living, saprophytic, or pathogenic, the latter causing disease in plants or animals.

细菌一种裂殖菌类的单细胞原核微生物，在结构形态、氧气、营养需求和运动方面都各有所不同，可以独立生存，也可是腐生或病原性的，后者可引起植物或动物的疾病

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。